公开(公告)号：WO2014172269A3

公开(公告)日：2015-10-29

申请号：PCT/US2014033978

申请日：2014-04-14

Applicant: BIO RAD LABORATORIES

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM ,  BHATT NEERAJ

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6456

Abstract: Systems and methods for simultaneously exciting a fluorescently labeled specimen and capturing fluorescent light emitted therefrom using a smartphone, tablet, or similar mobile computing device, are disclosed herein. The system includes a light-emitting diode ("LED") light source coupled with the smartphone to excite the specimen and an imaging device coupled with the smartphone to capture fluorescent light emitted from the specimen. The system further includes a hood adapted to be coupled with the smartphone that has an excitation filter configured to produce a first wavelength of electromagnetic radiation to strike the specimen when light from the LED light source passes through it and an emission filter configured to receive the light emitted from the specimen and to produce a second wavelength of electromagnetic radiation to be captured by the imaging device.

Abstract translation: 本文公开了同时激发荧光标记的样本并使用智能电话，平板电脑或类似的移动计算设备捕获从其发射的荧光的系统和方法。 该系统包括与智能手机耦合以激发样本的发光二极管（“LED”）光源和与智能手机耦合的成像装置以捕获从样品发射的荧光。 该系统还包括适于与智能电话耦合的罩，其具有激励滤波器，该激励滤波器被配置为产生第一波长的电磁辐射以在来自LED光源的光穿过其时撞击样本;以及发射滤波器，被配置为接收光 从样品发射并产生将被成像装置捕获的第二波长的电磁辐射。

公开(公告)号：EP3024843A4

公开(公告)日：2017-03-29

申请号：EP14830330

申请日：2014-07-24

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: C07K1/16 ,  A61K38/02 ,  C07K1/18

CPC classification number: C07K1/1136 ,  C07K1/22

Abstract: Compositions, methods, articles of manufacture, and kits are provided for storage and delivery of proteins.

Abstract translation: 组合物，方法，制造的物品和试剂盒提供了用于存储和蛋白质的交付。

公开(公告)号：EP2877483A4

公开(公告)日：2016-07-06

申请号：EP13823401

申请日：2013-07-25

Applicant: BIO RAD LABORATORIES

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: C07K1/04 ,  C07K1/14 ,  C12M1/00 ,  G01N27/447

CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

公开(公告)号：EP2986968A4

公开(公告)日：2017-03-22

申请号：EP15758608

申请日：2015-03-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: G01N21/00 ,  G01N21/75 ,  G01N33/559

CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

公开(公告)号：EP2986646A4

公开(公告)日：2017-04-12

申请号：EP14834424

申请日：2014-08-08

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM ,  OLECH LEE ,  BERKELMAN THOMAS ,  BELISLE CHRISTOPHER

IPC: G01N33/58 ,  G01N27/447 ,  G01N33/68

CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.