Abstract:
In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites.
Abstract:
Un método para determinar la presencia de anticuerpos de VIH en un fluido corporal, incluyendo: (a) contacto en condiciones que permitan la unión inmunoespecífica para formar una mezcla de reacción del fluido corporal con una composición que contenga por lo menos un polipéptido seleccionado del grupo consistente en las secuencias de aminoácidos: **(Ver fórmula)** donde W es un H del grupo amino terminal NH2 del polipéptido, o uno o más aminoácidos adicionales ligados al grupo amino terminal NH2 del polipéptido, seleccionándose los aminoácidos adicionales para facilitar el acoplamiento del polipéptido a una proteína portadora o a un soporte; X está ausente, Cys-Gly-Gly; o Lys-Lys, Y está ausente, Cys o uno o más aminoácidos añadidos para facilitar el acoplamiento; y Z es OH o NH; (b) detectar si se ha producido unión inmunoespecífica entre el polipéptido y un componente anticuerpo del fluido corporal formando un complejo inmune e indicando la detección del complejo inmune la presencia de anticuerpos de VIH en el fluido corporal.
Abstract:
Novel polypeptides, and recombinant polynucleotide sequences encoding the same, are provided. The polypeptides have substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
Abstract:
In sandwich-type immunoassays that capture a protein analyte between a capture antibody, typically bound to a solid phase, and a detection antibody that is coupled to a reporter group, the number of reporter groups associated with each molecule of analyte is increased by a variety of methods that utilize avidin-biotin-type binding in conjunction with such features as immunological binding to the reporter group on the detection antibody or multiple biotin-avidin-type binding sites.
Abstract:
Un método para determinar la presencia de anticuerpos para el virus de la inmunodeficiencia humana (HIV) en un fluido corporal, que comprende: (a) poner en contacto, en condiciones que permitan la unión inmunoespecífica para formar una mezcla de reacción, el fluido corporal con una composición que contiene al menos un polipéptido o proteína que consisten las siguientes secuencias de aminoácidos: (III) BRU 124F1X W-X-Lys-Ile-Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser- Arg-Asp-Pro-Leu-Trp-Lys-GIy-Pro-Ala-Lys-Leu-Leu-Trp- Lys-Gly-Glu-Gly-Ala-Val-Val-Ile-GIn-Asp-Asn-Ser-Asp- Ile-Lys-Y-Z (IV)BR 124F3X W-X-Lys-Ile-Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser- Arg-Asp-Pro-Leu-Trp-Lys-Gly-Pro-Ala-Lys-Leu-Leu-Trp- Lys-Gly-Glu-Gly-Ala-Val-Val-Ile-Gln-Asp-Asn-Y-Z (XIII) pol7-aa W-S-Tyr-Ser-Ala-Gly-Glu-Arg-Ile-Val-Asp-Ile-Ile-Ala-Thr- Asp-Ile-Gln-Thr-Lys-Glu-Leu-GIn-Lys-Gln-Ile-Thr-Lys-Ile- Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser-Arg-Asp-Pro- Leu-Trp-Lys-Gly-Pro-Ala-Lys-Leu-Leu-Trp-Lys-Gly-Glu- Gly-Ala-Val-Val-lle-Gln-Asp-Asn-Ser-Asp-lle-Lys-Val-Val- Pro-Arg-Arg-Lys-Ala-Lys-Ile-Ile-Arg-Asp-Tyr-Gly-Lys-Gln- Met-Ala-GIy-Asp-Asp-Y-Z
Abstract:
Novel polypeptides, and recombinant polynucleotide sequences encoding the same, are provided. The polypeptides have substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
Abstract:
Novel polypeptides, and recombinant polynucleotide sequences encoding the same, are provided. The polypeptides have substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.