公开(公告)号：JP2010104372A

公开(公告)日：2010-05-13

申请号：JP2009291496

申请日：2009-12-22

Applicant: Cepheid ,  セフィードCepheid

Inventor： TAYLOR MICHAEL T

IPC: C12Q1/68 ,  C12M1/33 ,  C12M1/42 ,  C12N1/00 ,  C12N1/06 ,  C12N13/00 ,  C12Q1/02 ,  C12Q1/70 ,  G01N1/28

CPC classification number: G01N1/286 ,  C12M47/06 ,  C12N1/066

Abstract: PROBLEM TO BE SOLVED: To provide an improved apparatus and method for disrupting cells or viruses to release nucleic acids therefrom. SOLUTION: The apparatus 10 for disrupting cells or viruses includes a container 18 having a chamber for holding the cells or viruses. The chamber is defined by at least one wall having an external surface to which is coupled a transducer device 38. The transducer device 38 vibrates at an operating frequency and amplitude sufficient to generate pressure waves or pressure pulses in the chamber. The transducer device 38 is coupled to the wall with a preload force sufficient to create a stress within the wall. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供用于破坏细胞或病毒以从其释放核酸的改进的装置和方法。 解决方案：用于破坏细胞或病毒的装置10包括具有用于保持细胞或病毒的室的容器18。 腔室由具有外表面的至少一个壁限定，耦合到换能器装置38.换能器装置38以足以产生腔室中的压力波或压力脉冲的工作频率和振幅振动。 换能器装置38以足以在壁内产生应力的预载力联接到壁上。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：WO0073412A3

公开(公告)日：2002-03-21

申请号：PCT/US0014738

申请日：2000-05-30

Applicant: CEPHEID

Inventor： PETERSEN KURT E ,  TAYLOR MICHAEL T ,  POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  BELGRADER PHILLIP ,  NORTHRUP M ALLEN ,  DORITY DOUGLAS B

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

Abstract translation: 用于从流体样品分离所需分析物的筒在样品流动路径中具有样品流动路径和裂解室（86）。 裂解室包含至少一个用于在样品流过裂解室时从样品捕获细胞或病毒的过滤器。 珠也设置在裂解室中，用于破裂细胞或病毒以从其中释放分析物。 分析物流动路径从裂解室延伸并从样品流动路径发散。 分析物流动路径优选地导致用于化学反应和光学检测分析物的反应室。 该盒还包括至少一个流量控制器（例如阀），用于在样品流过裂解室之后将样品引入废物室，并将从样品分离的分析物引导到分析物流动路径中。

公开(公告)号：WO0073413A2

公开(公告)日：2000-12-07

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：CA2462683C

公开(公告)日：2012-12-04

申请号：CA2462683

申请日：2002-10-03

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T

IPC: G01N1/28 ,  C12M1/33 ,  C12M1/42 ,  C12N1/00 ,  C12N1/06 ,  C12N13/00

Abstract: An apparatus (10) for disrupting cells or viruses comprises a container (18) having a chamber (40) for holding the cells or viruses. The chamber is defined by at least one wall (46, 86) having an external surface to which is coupled a transducer device (38). The transducer device (38) vibrates at an operating frequency and amplitude sufficient to generate pressure waves or pressure pulses in the chamber (40). The transducer device (38) is coupled to the wall (46, 86) with a preload force sufficient to create a stress within the wall (46, 86). The natural frequency of the wall (46, 86), when the wall is stressed by the preload force, is equal to the operating frequency of the transducer device (38) or differs from the operating frequency by less than 50% of the operating frequency.

公开(公告)号：AU5301000A

公开(公告)日：2000-12-18

申请号：AU5301000

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

公开(公告)号：WO0073413A9

公开(公告)日：2002-01-24

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：CA2373249C

公开(公告)日：2011-08-02

申请号：CA2373249

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  C12M3/08 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

公开(公告)号：CA2374423C

公开(公告)日：2013-04-09

申请号：CA2374423

申请日：2000-05-30

Applicant: CEPHEID

Inventor： PETERSEN KURT E ,  TAYLOR MICHAEL T ,  POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  BELGRADER PHILLIP ,  NORTHRUP M ALLEN ,  DORITY DOUGLAS B

IPC: C12M1/33 ,  C12M3/08 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

公开(公告)号：HK1068569A1

公开(公告)日：2005-04-29

申请号：HK05100978

申请日：2005-02-04

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T

IPC: B02C20060101 ,  C12M1/33 ,  C12M1/42 ,  C12N1/00 ,  C12N1/06 ,  C12N13/00 ,  G01N1/28

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The chamber is defined by at least one wall having an external surface for contacting a transducer device. The transducer device has a vibrating surface for contacting the wall and for vibrating at an operating frequency and amplitude sufficient to generate pressure waves or pressure pulses in the chamber. The transducer device is coupled to the wall with a preload force sufficient to create a stress within the wall. The natural frequency of the wall, when the wall is stressed by the preload force, is equal to the operating frequency of the transducer device or differs from the operating frequency by less than 50% of the operating frequency.

公开(公告)号：AU2002365128A1

公开(公告)日：2003-07-15

申请号：AU2002365128

申请日：2002-10-03

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T

IPC: C12M1/33 ,  C12M1/42 ,  C12N1/00 ,  C12N1/06 ,  C12N13/00 ,  G01N1/28

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The chamber is defined by at least one wall having an external surface for contacting a transducer device. The transducer device has a vibrating surface for contacting the wall and for vibrating at an operating frequency and amplitude sufficient to generate pressure waves or pressure pulses in the chamber. The transducer device is coupled to the wall with a preload force sufficient to create a stress within the wall. The natural frequency of the wall, when the wall is stressed by the preload force, is equal to the operating frequency of the transducer device or differs from the operating frequency by less than 50% of the operating frequency.