公开(公告)号：JPH02223546A

公开(公告)日：1990-09-05

申请号：JP18065189

申请日：1989-07-14

Applicant: KERNFORSCHUNGSANLAGE JUELICH ,  DEGUSSA

Inventor： KULA MARIA-REGINA ,  NIEDERMEYER UWE ,  STUERTZ INGEBORG MARIA

IPC: C07C255/12 ,  C12P13/00 ,  C12R1/00

公开(公告)号：WO2004005517A2

公开(公告)日：2004-01-15

申请号：PCT/EP0307148

申请日：2003-07-04

Applicant: DEGUSSA ,  KULA MARIA-REGINA ,  KRIEG LUTZ

Inventor： KULA MARIA-REGINA ,  KRIEG LUTZ

IPC: C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P13/04 ,  C12P41/00 ,  C12N15/01 ,  C12N15/11 ,  C12N15/63

CPC classification number: C12R1/41 ,  C12N9/78 ,  C12P13/04 ,  C12P41/006

Abstract: The present invention provides a new L-amidase from the organism Rhizobium huautlense. The invention also relates to the nucleic acids which code this enzyme and the vehicles, such as plasmids or microorganisms, containing these. A process for further improvement of these enzymes, their use and a whole cell catalyst containing the enzyme according to the invention are also disclosed.

Abstract translation: 本发明提供了一种来自生物体根瘤菌Rhitobium huautlense的新型L-酰胺酶。 本发明还涉及编码这种酶的核酸以及含有这些酶的载体，如质粒或微生物。 还公开了用于进一步改进这些酶的方法，它们的用途和含有根据本发明的酶的全细胞催化剂。

公开(公告)号：JPH11225784A

公开(公告)日：1999-08-24

申请号：JP34168798

申请日：1998-12-01

Applicant: DEGUSSA

Inventor： KULA MARIA-REGINA ,  POHL MARTINA DR ,  SLUSARCZYK HEIKE DR

IPC: C12N15/09 ,  C12N1/19 ,  C12N9/02 ,  C12N9/04 ,  C12N15/00 ,  C12N15/53 ,  C12P41/00 ,  C12R1/72

Abstract: PROBLEM TO BE SOLVED: To obtain the subject new mutant consisting of a mutant of formic acid dehydrogenase derived from Candida boidinii, with the sulfur-contg. amino acids in the enzyme exchanged by sulfur-free amino acids, improved in stability to oxidation, and usable for e.g. the regeneration of NADH. SOLUTION: This new mutant of formic acid dehydrogenase (rec-FDH) derived from Candida boidinii and sensitive to flocculation and oxidation compared to rec-FDH and the corresponding wild-type enzyme, is such one that the sulfur- contg. amino acids in the rec-FDH have been exchanged with sulfur-free amino acids either mutually independently, individually or together, by targeted mutagenesis. The mutant is used for e.g. the regeneration of expensive NADH in the production of L-amino acids from the corresponding α-keto acids in the presence of amino acid dehydrogenase and NADH as coenzyme. The mutant is obtained by amplifying FDH gene cloned from the genomic DNA of Candida boidinii with a mutagenic primer.

公开(公告)号：JP2003180383A

公开(公告)日：2003-07-02

申请号：JP2002275723

申请日：2002-09-20

Applicant: DEGUSSA

Inventor： SLUSARCZYK HEIKE DR ,  FELBER STEFAN ,  KULA MARIA-REGINA ,  POHL MARTINA DR

IPC: C12N15/09 ,  C12N1/19 ,  C12N9/02 ,  C12N15/53 ,  C12R1/72

Abstract: PROBLEM TO BE SOLVED: To avoid expensive postfeed of FDH so as to reduce a manufacturing cost by raising catalytic activity and stability of rec-FDH. SOLUTION: This variant is stable and/or active to wild type rec-FDH and natural wild type enzyme from Candida boidinii and has amino acid exchange C23S or C23S/C262A and one or more amino acid exchanges of E18D, K35R, D149E, E151D, R178S, R178G, K206R, F285Y, F285S, T315N and K356E. COPYRIGHT: (C)2003,JPO

公开(公告)号：JP2002142794A

公开(公告)日：2002-05-21

申请号：JP2001311652

申请日：2001-10-09

Applicant: DEGUSSA

Inventor： BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ ,  VERSECK STEFAN ,  KULA MARIA-REGINA

IPC: C12P13/04 ,  C12N9/90 ,  C12P41/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for racemization of N-carbamoylamino acid more improved than a conventional technique by using N-acetylamino acid racemase. SOLUTION: N-Acetylamino acid racemase(AAR) derived from Amycolatopsis orientalis sp. lurida is used.

公开(公告)号：JPH025885A

公开(公告)日：1990-01-10

申请号：JP1519989

申请日：1989-01-26

Applicant: KERNFORSCHUNGSANLAGE JUELICH ,  DEGUSSA

Inventor： NIEDERMEYER UWE ,  KRAGL UDO ,  KULA MARIA-REGINA ,  WANDREY CHRISTIAN ,  MAKRYALEAS KYRIAKOS ,  DRAUZ KARLHEINZ

IPC: C07C253/32 ,  C07C255/12 ,  C07C255/36 ,  C12P13/00

公开(公告)号：JPS637782A

公开(公告)日：1988-01-13

申请号：JP15360487

申请日：1987-06-22

Applicant: DEGUSSA ,  BIOTECHNOLOG FORSCHUNG GMBH

Inventor： KULA MARIA-REGINA ,  HUMMEL WERNER ,  SCHUETTE HORST ,  LEUCHTENBERGER WOLFGANG

IPC: C12N9/80 ,  C12P13/04 ,  C12R1/13

公开(公告)号：WO2005093080A3

公开(公告)日：2006-04-27

申请号：PCT/EP2005002134

申请日：2005-03-01

Applicant: DEGUSSA

Inventor： OSSWALD STEFFEN ,  VERSECK STEFAN ,  DEITING UTA ,  WECKBECKER CHRISTOPH ,  HUTHMACHER KLAUS ,  BINDER MICHAEL ,  KULA MARIA-REGINA ,  ODENDAHL KONRAD

IPC: C12P13/02 ,  C07K14/47 ,  C12N9/78 ,  C12N9/88

CPC classification number: C07K14/47 ,  C12N9/88 ,  C12P13/02

Abstract: The invention relates to a polynucleotide cluster of Rhodococcus, containing nucleotide sequences coding for polypeptides having the activity of a nitrile hydratase, an auxiliary protein P15K activating said enzyme, and a cobalt transporter. The invention also relates to micro-organisms transformed by said cluster, in which the nucleotide sequences coding for said proteins are reinforced, and to the use of the transformed micro-organisms for producing amides from nitriles.

Abstract translation: 本发明涉及从红球菌一个Polynucleotidcluster，具有这种酶激活辅助蛋白P15K和转运编码钴核苷酸序列的腈水合酶的活性，所述多肽包含转化的，其中用于这些蛋白质的编码核苷酸序列的至少扩增的微生物和利用所述转化的微生物用于生产 从腈酰胺。

公开(公告)号：JP2003225094A

公开(公告)日：2003-08-12

申请号：JP2002354266

申请日：2002-12-05

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  DRAUZ KARLHEINZ ,  BOMMARIUS ANDREAS ,  KULA MARIA-REGINA ,  KRIEG LUTZ ,  SLUSARCZYK HEIKE DR ,  ANSORGE-SCHUMACHER MARION

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/80 ,  C12N9/90 ,  C12N15/55 ,  C12P7/40 ,  C12P13/04 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a novel amidase suitable for an industrial means and for producing an enantio-rich compound from a racemic body. SOLUTION: Disclosed is a novel amidase derived from an organism, Variovorax paradoxus. COPYRIGHT: (C)2003,JPO

公开(公告)号：JP2001046088A

公开(公告)日：2001-02-20

申请号：JP2000222928

申请日：2000-07-24

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  KULA MARIA-REGINA ,  BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/90 ,  C12N15/61 ,  C12N15/80 ,  C12P13/04 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/04

Abstract: PROBLEM TO BE SOLVED: To obtain the subject new enzyme comprising an N-acetylamino acid racemase from Amycolatopsis orientalis subspecies lurida and useful for the production etc. of an optically active amino acid useful for the production, etc., of a chiral bioactive substance. SOLUTION: This new N-acetylamino acid racemase is derived from Amycolatopsis orientalis subspecies lurida and is useful for the production, etc. of an optically pure amino acid used for the production, etc. of an extra- intestinal ingestive and a chiral bioactive agent with small dependence on heavy metal ions. The N-acetylamino acid racemase is obtained by culturing an Actinomyces strain Amycolatopsis orientalis subspecies lurida, harvesting the cultured strain, then extracting a genomic DNA, carrying out a polymerase chain reaction(PCR) using a primer comprising a partial sequence, conducting the cloning, integrating the resultant gene into an expression vector, transforming a host cell and culturing the prepared tansformant.