公开(公告)号：JP2001037473A

公开(公告)日：2001-02-13

申请号：JP2000196986

申请日：2000-06-29

Applicant: DEGUSSA

Inventor： BOMMARIUS ANDREAS ,  ESTLER MANFRED ,  ANDREAS KRUGE ,  WERNER HELGE ,  VOLLMER HELGA DR ,  BOCK HANS-GEORG ,  SCHLOEDER JOHANNES P ,  KOSTINA EKATERINA

IPC: C12N9/00 ,  C12Q1/00

Abstract: PROBLEM TO BE SOLVED: To determine the process stability of an enzyme for grasping the specific amount of the enzyme by adopting a temperature-time profile of a reaction to be tested in a continuously operating non-stationary derivative type reactor, etc., and obtaining characteristic parameters, etc., under a specific condition. SOLUTION: This method for determining the process stability of an enzyme is provided by adopting a temperature-time profile of a reaction to be tested in a continuously operating non-stationary derivative type reactor, circulating type reactor or agitating reactor and obtaining characteristic activation parameter and stability parameter preferably by (i) the calculation of the Arrhenius curve for the deactivation by using the experimental measurements of 8 starting parameters and initial values, (ii) the mathematical calculation of a special temperature-time profile as a standard value of a new experiment for adopting a reaction rate-time curve, (iii) the improvement of the parameter evaluation based on the new experimental data, and (iv) the calculation of optimal temperatures of parameters related to the process such as a total turnover number, a half life, etc.

公开(公告)号：JP2002142794A

公开(公告)日：2002-05-21

申请号：JP2001311652

申请日：2001-10-09

Applicant: DEGUSSA

Inventor： BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ ,  VERSECK STEFAN ,  KULA MARIA-REGINA

IPC: C12P13/04 ,  C12N9/90 ,  C12P41/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for racemization of N-carbamoylamino acid more improved than a conventional technique by using N-acetylamino acid racemase. SOLUTION: N-Acetylamino acid racemase(AAR) derived from Amycolatopsis orientalis sp. lurida is used.

公开(公告)号：WO02077212A3

公开(公告)日：2002-12-12

申请号：PCT/EP0201840

申请日：2002-02-21

Applicant: DEGUSSA

Inventor： DRAUZ KARLHEINZ ,  MAY OLIVER ,  BOMMARIUS ANDREAS ,  SYLDATK CHRISTOPH ,  ALTENBUCHNER JOSEF ,  WERNER MARKUS ,  SIEMANN-HERZBERG MARTIN

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/80 ,  C12N15/55 ,  C12P13/04 ,  C12P41/00 ,  C12N1/20 ,  C12N15/10 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/06

CPC classification number: C12N9/80 ,  C12N2310/111 ,  C12P13/04 ,  C12P41/009 ,  G01N2333/195

Abstract: The present invention relates to a new D-carbamoylase and the gene sequences which code for this from the organism Arthrobacter crystallopoietes DSM 20117. Plasmids, vectors, microorganisms, particular primers and specific possible uses of the enzymes according to the invention are also mentioned. The invention moreover describes a new process for the discovery of enzymes which can be employed in a process for the preparation of D-amino acids starting from 5'-substituted hydantoins.

Abstract translation: 本发明涉及一种新的D-氨基甲酰基酶和从生物体节杆菌结晶洗脱剂DSM 20117中为此编码的基因序列。还提及了根据本发明的酶的质粒，载体，微生物，特定引物和具体可能的用途。 本发明还描述了用于发现酶的新方法，其可用于从5'-取代的乙内酰脲开始制备D-氨基酸的方法。

公开(公告)号：WO9848032A3

公开(公告)日：1999-01-28

申请号：PCT/EP9801996

申请日：1998-04-06

Applicant: DEGUSSA ,  UNIV MASSACHUSETTS ,  TIRRELL DAVID A ,  DEL GROSSO MICHAEL ,  SIEGMEIER RAINER ,  BOMMARIUS ANDREAS

Inventor： TIRRELL DAVID A ,  DEL GROSSO MICHAEL ,  SIEGMEIER RAINER ,  BOMMARIUS ANDREAS

IPC: C07K14/00 ,  C12P21/02 ,  C12P21/00

CPC classification number: C12P21/02 ,  C07K14/00

Abstract: The invention provides Poly-(α-L-aspartic acid) (= poly-L-α-Asp), Poly-(α-L-glutamic acid) and new copolymers of L-Asp and L-Glu with essentially α-linkages as well as salts of said polymers and copolymers. A method for producing said polyamino acids comprises employing an oligonucleotide construct encoding the sequence of the desired polymer, cloning the oligonucleotide into a plasmid, transformation of host cells with the plasmid and expression of the genetic information. Because of their good biological degradability the new poly-L-α-amino acids can be used as co-builders, sequestering agents and corrosion inhibitors.

公开(公告)号：WO0123535A3

公开(公告)日：2001-05-25

申请号：PCT/EP0008580

申请日：2000-09-02

Applicant: DEGUSSA ,  UNIV STUTTGART ,  ROCHE DIAGNOSTICS GMBH ,  ALTENBUCHNER JOSEF ,  MATTES RALF ,  PIETZSCH MARKUS ,  SYLDATK CHRISTOPH ,  WIESE ANJA ,  BOMMARIUS ANDREAS ,  TISCHER WILHELM

Inventor： ALTENBUCHNER JOSEF ,  MATTES RALF ,  PIETZSCH MARKUS ,  SYLDATK CHRISTOPH ,  WIESE ANJA ,  BOMMARIUS ANDREAS ,  TISCHER WILHELM

IPC: C12N9/90 ,  C12P41/00 ,  C12N15/61

CPC classification number: C12N9/90 ,  C12P41/009

Abstract: The instant invention is directed to a rec-hydantoin-racemase from Arthrobacter aurescens DSM 3747. Furthermore, the gene encoding for the racemase and plasmids, vectors and microorganisms comprising this gene are to be protected. Use in a process for the production of amino carboxylic acids or derivatives thereof.

Abstract translation: 本发明涉及来自Arthrobacter aurescens DSM 3747的rec-乙内酰脲 - 外消旋酶。此外，编码消旋酶的基因和质粒，包含该基因的载体和微生物将被保护。 用于生产氨基羧酸或其衍生物的方法中。

公开(公告)号：WO9729837A3

公开(公告)日：1997-10-09

申请号：PCT/EP9700734

申请日：1997-02-17

Applicant: DEGUSSA ,  WANDREY CHRISTIAN ,  KRAGL UDO ,  BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ

Inventor： WANDREY CHRISTIAN ,  KRAGL UDO ,  BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ

IPC: B01D61/02 ,  B01J4/04 ,  B01J19/24 ,  C07C51/42 ,  C12M1/40 ,  C12M1/12

CPC classification number: C07C51/42 ,  B01D61/02 ,  B01J4/04 ,  B01J19/2475

Abstract: In catalytic reactions and reactions requiring synthesis-promoters, it is useful to be able to separate the promoter from the product formed and thus make it available for recycling. One possible way of achieving this separation is by immobilising the synthesis-promoter on special carriers. This, however, often reduces the contact area between the promoter and carrier and thus markedly reduces the reactivity of the promoter or alters its mode of action. The immobilisation process itself may also present problems in terms of working time spent and catalyst loss. If the product and promoter in a catalytic reaction are separated by filtration using a nanofiltration or reverse osmosis membrane, it is possible, even when the weight differences between promoter and product are relatively small and provided that the retension differences are sufficient and the associated recovery rate of the promoter therefore high, to dispense with separation based on immobilisation techniques. The process proposed can be used for separating reaction mixtures for catalytic reactions with the aim of efficiently producing organic synthesis products or optically active organic compounds.

公开(公告)号：JP2003225094A

公开(公告)日：2003-08-12

申请号：JP2002354266

申请日：2002-12-05

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  DRAUZ KARLHEINZ ,  BOMMARIUS ANDREAS ,  KULA MARIA-REGINA ,  KRIEG LUTZ ,  SLUSARCZYK HEIKE DR ,  ANSORGE-SCHUMACHER MARION

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/80 ,  C12N9/90 ,  C12N15/55 ,  C12P7/40 ,  C12P13/04 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a novel amidase suitable for an industrial means and for producing an enantio-rich compound from a racemic body. SOLUTION: Disclosed is a novel amidase derived from an organism, Variovorax paradoxus. COPYRIGHT: (C)2003,JPO

公开(公告)号：JP2001294540A

公开(公告)日：2001-10-23

申请号：JP2001015825

申请日：2001-01-24

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： LAUE STEPHAN ,  LIESE ANDREAS ,  WANDREY CHRISTIAN ,  BURKHARDT OLAF DR ,  WOELTINGER JENS ,  BOMMARIUS ANDREAS ,  HENNIGES HANS ,  PHILIPPE JEAN-LOUIS ,  KARAU ANDREAS ,  DRAUZ KARLHEINZ

IPC: B01J31/22 ,  B01J31/16 ,  B01J31/18 ,  B01J31/24 ,  B01J31/28 ,  C07B31/00 ,  C07B53/00 ,  C07B61/00 ,  C07F9/50 ,  C07F15/00 ,  C08G77/398

Abstract: PROBLEM TO BE SOLVED: To enable the lowering of production cost by providing the use of molecular weight-enlarged, homogeneously soluble catalysts in a membrane reactor in a process for the asymmetric, continuous hydrogenation of C=C, C=N or C=0 double bonds, novel molecular weight-enlarged ligands, and asymmetric hydrogenation catalysts. SOLUTION: The ligands, catalysts, active centre, and their uses, comprises ligands such as polyacrylates, polystyrene, polysiloxanes or polyacrylates as molecular weight-enlarging agents, having an average molecular weight of 1,000 to 1,000, 000 g/mole, a metal or metal ion selected from Ru, Rh, Ir, Pd, Ni, Pt, and Co, and a representative example is N,N'-bis(2- diphenylphosphanylbenzyl) cyclohexyl-1,2-diamine as the asymmetric synthesis active center of the ligand, or the like.

公开(公告)号：JP2001288218A

公开(公告)日：2001-10-16

申请号：JP2001013677

申请日：2001-01-22

Applicant: DEGUSSA

Inventor： KRIMMER HANS-PETER ,  WOELTINGER JENS ,  BURKHARDT OLAF DR ,  KLEMENT INGO DR ,  HENNIGES HANS ,  DRAUZ KARLHEINZ ,  BOMMARIUS ANDREAS ,  PHILIPPE JEAN-LOUIS ,  KARAU ANDREAS

IPC: C07D263/10 ,  B01J31/18 ,  B01J31/22 ,  B01J31/24 ,  C07B53/00 ,  C07D207/08 ,  C07D207/16 ,  C07D209/52 ,  C07D263/28 ,  C07D413/14 ,  C08F8/30 ,  C08F8/40 ,  C08F30/00 ,  C08G85/00

Abstract: PROBLEM TO BE SOLVED: To provide a ligand separable between a product and a catalyst relating to a membrane used and other ligands relating to the catalyst for the purpose of excluding problems that the catalyst is deactivated accompanying reaction progress. SOLUTION: In such case of a homogeneous soluble ligand having increased molecular weight for a hydrogenation catalyst having a homochiral active center, a specific linker may be inserted between the active center and a polymer skeleton, further the ligand is transferred into the catalyst and preferably may be used just like continuously or continuously in a membrane type reactor for an organic synthesis.

公开(公告)号：JP2001046088A

公开(公告)日：2001-02-20

申请号：JP2000222928

申请日：2000-07-24

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  KULA MARIA-REGINA ,  BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/90 ,  C12N15/61 ,  C12N15/80 ,  C12P13/04 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/04

Abstract: PROBLEM TO BE SOLVED: To obtain the subject new enzyme comprising an N-acetylamino acid racemase from Amycolatopsis orientalis subspecies lurida and useful for the production etc. of an optically active amino acid useful for the production, etc., of a chiral bioactive substance. SOLUTION: This new N-acetylamino acid racemase is derived from Amycolatopsis orientalis subspecies lurida and is useful for the production, etc. of an optically pure amino acid used for the production, etc. of an extra- intestinal ingestive and a chiral bioactive agent with small dependence on heavy metal ions. The N-acetylamino acid racemase is obtained by culturing an Actinomyces strain Amycolatopsis orientalis subspecies lurida, harvesting the cultured strain, then extracting a genomic DNA, carrying out a polymerase chain reaction(PCR) using a primer comprising a partial sequence, conducting the cloning, integrating the resultant gene into an expression vector, transforming a host cell and culturing the prepared tansformant.