公开(公告)号：JP2002142794A

公开(公告)日：2002-05-21

申请号：JP2001311652

申请日：2001-10-09

Applicant: DEGUSSA

Inventor： BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ ,  VERSECK STEFAN ,  KULA MARIA-REGINA

IPC: C12P13/04 ,  C12N9/90 ,  C12P41/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for racemization of N-carbamoylamino acid more improved than a conventional technique by using N-acetylamino acid racemase. SOLUTION: N-Acetylamino acid racemase(AAR) derived from Amycolatopsis orientalis sp. lurida is used.

公开(公告)号：JP2002325598A

公开(公告)日：2002-11-12

申请号：JP2001312893

申请日：2001-10-10

Applicant: DEGUSSA

Inventor： BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ ,  VERSECK STEFAN

IPC: C12N15/09 ,  C07C227/20 ,  C07C323/59 ,  C12P13/04 ,  C12P13/12 ,  C12P41/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for producing an optically concentrated amino acid from the racemate mixture of the amino acid protected with a urethane or carbamoyl protecting group. SOLUTION: This method for producing the enantiomer-concentrated amino acid is characterized by reacting a compound of formula (I) (X is O or NH; R is CH3 , CH3 CH2 , tert-butyl, or benzyl, provided that R may be H, when X is NH; R is the α-group of a natural or synthetic amino acid) with an enzyme exhibiting an N-acetylamino acid racemase activity (AAR) in the presence of an enzyme exhibiting an amino acid acylase activity or then with the enzyme exhibiting an amino acid acylase activity.

公开(公告)号：JP2003225094A

公开(公告)日：2003-08-12

申请号：JP2002354266

申请日：2002-12-05

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  DRAUZ KARLHEINZ ,  BOMMARIUS ANDREAS ,  KULA MARIA-REGINA ,  KRIEG LUTZ ,  SLUSARCZYK HEIKE DR ,  ANSORGE-SCHUMACHER MARION

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/80 ,  C12N9/90 ,  C12N15/55 ,  C12P7/40 ,  C12P13/04 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a novel amidase suitable for an industrial means and for producing an enantio-rich compound from a racemic body. SOLUTION: Disclosed is a novel amidase derived from an organism, Variovorax paradoxus. COPYRIGHT: (C)2003,JPO

公开(公告)号：JP2001046088A

公开(公告)日：2001-02-20

申请号：JP2000222928

申请日：2000-07-24

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  KULA MARIA-REGINA ,  BOMMARIUS ANDREAS ,  DRAUZ KARLHEINZ

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/90 ,  C12N15/61 ,  C12N15/80 ,  C12P13/04 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/04

Abstract: PROBLEM TO BE SOLVED: To obtain the subject new enzyme comprising an N-acetylamino acid racemase from Amycolatopsis orientalis subspecies lurida and useful for the production etc. of an optically active amino acid useful for the production, etc., of a chiral bioactive substance. SOLUTION: This new N-acetylamino acid racemase is derived from Amycolatopsis orientalis subspecies lurida and is useful for the production, etc. of an optically pure amino acid used for the production, etc. of an extra- intestinal ingestive and a chiral bioactive agent with small dependence on heavy metal ions. The N-acetylamino acid racemase is obtained by culturing an Actinomyces strain Amycolatopsis orientalis subspecies lurida, harvesting the cultured strain, then extracting a genomic DNA, carrying out a polymerase chain reaction(PCR) using a primer comprising a partial sequence, conducting the cloning, integrating the resultant gene into an expression vector, transforming a host cell and culturing the prepared tansformant.

公开(公告)号：WO2005093080A3

公开(公告)日：2006-04-27

申请号：PCT/EP2005002134

申请日：2005-03-01

Applicant: DEGUSSA

Inventor： OSSWALD STEFFEN ,  VERSECK STEFAN ,  DEITING UTA ,  WECKBECKER CHRISTOPH ,  HUTHMACHER KLAUS ,  BINDER MICHAEL ,  KULA MARIA-REGINA ,  ODENDAHL KONRAD

IPC: C12P13/02 ,  C07K14/47 ,  C12N9/78 ,  C12N9/88

CPC classification number: C07K14/47 ,  C12N9/88 ,  C12P13/02

Abstract: The invention relates to a polynucleotide cluster of Rhodococcus, containing nucleotide sequences coding for polypeptides having the activity of a nitrile hydratase, an auxiliary protein P15K activating said enzyme, and a cobalt transporter. The invention also relates to micro-organisms transformed by said cluster, in which the nucleotide sequences coding for said proteins are reinforced, and to the use of the transformed micro-organisms for producing amides from nitriles.

Abstract translation: 本发明涉及从红球菌一个Polynucleotidcluster，具有这种酶激活辅助蛋白P15K和转运编码钴核苷酸序列的腈水合酶的活性，所述多肽包含转化的，其中用于这些蛋白质的编码核苷酸序列的至少扩增的微生物和利用所述转化的微生物用于生产 从腈酰胺。

公开(公告)号：WO2004067738A2

公开(公告)日：2004-08-12

申请号：PCT/EP2004000338

申请日：2004-01-17

Applicant: DEGUSSA ,  SYLDATK CHRISTOPH ,  BRANDAO PEDRO ,  BULL ALAN T ,  VERSECK STEFAN ,  ERFURTH JEANNETTE ,  BUCHHOLZ STEFAN ,  OSSWALD STEFFEN ,  DRAUZ KARLHEINZ

Inventor： SYLDATK CHRISTOPH ,  BRANDAO PEDRO ,  BULL ALAN T ,  VERSECK STEFAN ,  ERFURTH JEANNETTE ,  BUCHHOLZ STEFAN ,  OSSWALD STEFFEN ,  DRAUZ KARLHEINZ

IPC: C12N9/88 ,  C12P13/02 ,  C12R1/01

CPC classification number: C12P13/02 ,  C12N9/88

Abstract: The present invention is directed to polypeptides exhibiting nitrile hydratase activity and the respective encoding nucleic acids from Rhodococcus erythropolis. Furthermore, micoorganisms, plasmids and vectors comprising the polypetides are also embraced by this invention.

Abstract translation: 本发明涉及显示腈水合酶活性的多肽和来自红串红球菌的相应编码核酸。 此外，包含多肽的微生物，质粒和载体也包含在本发明中。

公开(公告)号：WO2009056423A3

公开(公告)日：2009-07-09

申请号：PCT/EP2008063150

申请日：2008-10-01

Applicant: EVONIK DEGUSSA GMBH ,  VERSECK STEFAN ,  SCHAFFER STEFFEN ,  FREITAG WERNER ,  SCHMIDT FRIEDRICH GEORG ,  ORSCHEL MATTHIAS ,  GRUND GERDA ,  SCHMIDT WILFRIED ,  BAHL HUBERT JOHANNES ,  FISCHER RALF-JOERG ,  MAY ANTJE ,  DUERRE PETER ,  LEDERLE SIMONE

Inventor： VERSECK STEFAN ,  SCHAFFER STEFFEN ,  FREITAG WERNER ,  SCHMIDT FRIEDRICH GEORG ,  ORSCHEL MATTHIAS ,  GRUND GERDA ,  SCHMIDT WILFRIED ,  BAHL HUBERT JOHANNES ,  FISCHER RALF-JOERG ,  MAY ANTJE ,  DUERRE PETER ,  LEDERLE SIMONE

IPC: C12P7/28

CPC classification number: C12P7/28 ,  C12N9/16 ,  C12N9/93 ,  C12Y301/02 ,  C12Y301/02018

Abstract: The invention describes a process for the preparation of acetone starting from acetyl-coenzyme A comprising the process steps A. enzymatic conversion of acetyl-CoA to give acetoacetyl-CoA; B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA; and C. decarboxylation of acetoacetate to give acetone and CO2, which process is characterized in that, in process step B, the coenzyme A is not transferred to an acceptor molecule. Moreover, surprisingly, process step B is catalyzed by enzymes from the classes acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.

Abstract translation: 本发明描述了一种用于从乙酰辅酶A，其包含乙酰-CoA至乙酰乙酰基-CoA的A.酶转化的步骤的生产丙酮的方法; 乙酰乙酰CoA的B.酶转化为乙酰乙酸和CoA; 和C.乙酰丙酮和CO 2，其特征在于，所述辅酶A不传输到在方法步骤B.受体分子的脱羧 此外，出人意料的是，过程步骤B通过酰基辅酶A硫酯酶的类的酶催化，酰基-CoA合成酶或酰基辅酶A硫激酶。

公开(公告)号：DE102007005072A1

公开(公告)日：2008-08-07

申请号：DE102007005072

申请日：2007-02-01

Applicant: EVONIK DEGUSSA GMBH ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： VERSECK STEFAN ,  HAEGER HARALD ,  KARAU ANDREAS ,  EGGELING LOTHAR ,  SAHM HERMANN

IPC: C12N1/00

公开(公告)号：BRPI0509023A

公开(公告)日：2007-08-07

申请号：BRPI0509023

申请日：2005-03-01

Applicant: DEGUSSA

Inventor： OSSWALD STEFFEN ,  VERSECK STEFAN ,  DEITING UTA ,  WECKBECKER CHRISTOPH ,  HUTHMACHER KLAUS ,  BINDER MICHAEL ,  KULA MARIA-REGINA ,  ODENDAHL KONRAD

IPC: C12P13/02 ,  C07K14/47 ,  C12N9/78 ,  C12N9/88

Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

公开(公告)号：DE102004013842A1

公开(公告)日：2005-10-13

申请号：DE102004013842

申请日：2004-03-20

Applicant: DEGUSSA

Inventor： VERSECK STEFAN ,  LIEBETON KLAUS ,  ECK JUERGEN

IPC: C12N9/88 ,  C12Q1/68 ,  C12N15/60

Abstract: The present invention relates to a nucleic acid sequence which encodes a protein having the activity of a nitrile hydratase, wherein the nucleic acid sequence comprises (a) the first nucleic acid sequence of any one of SEQ ID NOs: 49, 51, 41, 39, 43, 37, 55, 47 or 53, and a second nucleic acid sequence of any one of SEQ ID NOs: 71, 73, 65, 63, 67, 77, 69, and 75 ; (b) the first nucleic acid sequence encoding a first amino acid sequence of any one of SEQ ID NOs: 50, 52, 42, 40, 44, 38, 56, 48 or 54, and a second nucleic acid sequence encoding a second amino acid sequence of any one of SEQ ID NOs: 72, 74, 66, 64, 68, 78, 70, and 76; or (c) a nucleic acid sequence, which is at least 70% identical to the nucleic acid sequence of (a) or (b).