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    MULTIFUNCTIONAL PROBE-PRIMERS
    1.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A3

    公开(公告)日：2013-01-10

    申请号：PCT/US2012023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORP ,  LIVAK KENNETH J

    Inventor： LIVAK KENNETH J

    IPC: C12Q1/68 ,  C07H21/00

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选的索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'取向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    DETECTION OF GENE DUPLICATIONS
    4.
    发明申请
    DETECTION OF GENE DUPLICATIONS 审中-公开
    检测基因重复

    公开(公告)号：WO2005113818A2

    公开(公告)日：2005-12-01

    申请号：PCT/US2005016395

    申请日：2005-05-11

    Applicant: APPLERA CORP ,  LIVAK KENNETH J ,  STEVENS JUNKO ,  LAZARUK KATHERINE D ,  ZIEGLE JANET S ,  WONG LILY Y

    Inventor： LIVAK KENNETH J ,  STEVENS JUNKO ,  LAZARUK KATHERINE D ,  ZIEGLE JANET S ,  WONG LILY Y

    IPC: C12Q1/68

    CPC classification number: G06F19/18 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q2600/156 ,  C12Q2563/107 ,  C12Q2545/101 ,  C12Q2565/101 ,  C12Q2563/173 ,  C12Q2565/1015

    Abstract: Methods of detecting a candidate genetic anomaly such as a candidate duplication in a genome are disclosed. The methods comprise quantifying fluorogenic assays for alleles of a genetic locus from a plurality of individual genomes, identifying ranges of fluorescent intensities indicative of individual genomes homozygous for a first allele, homozygous for a second allele, or heterozygous for both alleles, and identifying individual genomes in which the fluorescence intensities are outside the range of intensities indicative of homozygosity or heterozygosity for the genetic locus.

    Abstract translation: 公开了检测候选遗传异常（例如基因组中候选重复）的方法。 所述方法包括定量来自多个单个基因组的遗传基因座的等位基因的荧光测定，鉴定指示第一等位基因纯合的单个基因组的荧光强度的范围，对于第二等位基因纯合，或对于两个等位基因是杂合的，以及鉴定个体基因组 其中荧光强度在表示遗传基因座的纯合性或杂合性的强度范围之外。

    LIGATION AND AMPLIFICATION REACTIONS FOR DETERMINING TARGET MOLECULES
    5.
    发明申请
    LIGATION AND AMPLIFICATION REACTIONS FOR DETERMINING TARGET MOLECULES 审中-公开
    用于确定目标分子的放射反应和放大反应

    公开(公告)号：WO2005098040A8

    公开(公告)日：2005-12-01

    申请号：PCT/US2005010184

    申请日：2005-03-24

    Applicant: APPLERA CORP ,  ANDERSEN MARK R ,  LIVAK KENNETH J ,  BROOMER ADAM ,  CHEN CAIFU

    Inventor： ANDERSEN MARK R ,  LIVAK KENNETH J ,  BROOMER ADAM ,  CHEN CAIFU

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2525/191 ,  C12Q2525/185 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2521/313 ,  C12Q2537/143 ,  C12Q2525/161

    Abstract: The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.

    Abstract translation: 本发明涉及用于使用编码和解码反应检测至少一个样品中靶多核苷酸序列和蛋白质的存在或不存在（或定量）的方法，试剂和试剂盒。 当特定靶多核苷酸例如存在于样品中时，在包含可寻址引物部分的编码反应中形成反应产物。 可以在解码扩增反应中使用至少一个标记探针和至少一个地址引物，从而根据序列是否存在提供可检测的信号值。 在一些实施方案中，编码包含与接头探针的连接反应，并分析单核苷酸多态性（SNP）。

    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME
    6.
    发明申请
    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
    DNA序列方法和检测器及其执行系统

    公开(公告)号：WO2011082419A3

    公开(公告)日：2012-02-09

    申请号：PCT/US2011020117

    申请日：2011-01-04

    Applicant: LIFE TECHNOLOGIES CORP ,  SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/50

    CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    Abstract translation: 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔也是制备它们的方法。 在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

    METHODS OF ANALYZING BINDING INTERACTIONS
    7.
    发明申请
    METHODS OF ANALYZING BINDING INTERACTIONS 审中-公开
    分析结合相互作用的方法

    公开(公告)号：WO2008005674A3

    公开(公告)日：2008-12-24

    申请号：PCT/US2007071270

    申请日：2007-06-14

    Applicant: APPLERA CORP ,  SUN HONGYE ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  LIVAK KENNETH J

    IPC: G01N33/53

    CPC classification number: G01N33/566 ,  G01N33/48721

    Abstract: The present disclosure relates to methods of analyzing binding interactions between a binding component and a receptor component by translocating unbound and any bound components through a pore and detecting the unbound and bound components.

    Abstract translation: 本公开内容涉及通过将未结合的组分和任何结合的组分通过孔移位并检测未结合和结合的组分来分析结合组分和受体组分之间的结合相互作用的方法。

    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES
    8.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES 审中-公开
    用于检测蛋白质聚集体的方法，组合物和试剂盒

    公开(公告)号：WO2007128004A2

    公开(公告)日：2007-11-08

    申请号：PCT/US2007068046

    申请日：2007-05-02

    Applicant: APPLERA CORP ,  RUFF DAVID W ,  SHANNON MARK E ,  LIVAK KENNETH J ,  GUEGLER KARL J ,  HENNESSY KEVIN M

    Inventor： RUFF DAVID W ,  SHANNON MARK E ,  LIVAK KENNETH J ,  GUEGLER KARL J ,  HENNESSY KEVIN M

    IPC: C07H21/00 ,  C12N9/00

    CPC classification number: C12Q1/6876 ,  C07K14/47 ,  C07K14/4711 ,  C12Q1/6816 ,  C12Q2533/107

    Abstract: The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.

    Abstract translation: 本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。 在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。 然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。 在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。

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