公开(公告)号：EP3103884A4

公开(公告)日：2017-08-23

申请号：EP15746728

申请日：2015-01-29

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIMOTO NORIHIKO ,  EDA SOUJI ,  ASAKURA MASAHIRO ,  ABE KANAKO ,  UEHARA HIROTSUGU ,  KAMEI KAZUMASA ,  UESAKA YOSHIHIKO ,  OKU YUICHI ,  SHIBAHARA YUSUKE

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09

CPC classification number: C12Q1/6832 ,  C12Q2537/162 ,  C12Q2565/133 ,  C12Q2565/137

公开(公告)号：EP2192184A4

公开(公告)日：2011-08-17

申请号：EP08828171

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND INC

Inventor： YAMASAKI SHINJI ,  SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

CPC classification number: C12Q1/689 ,  C07K16/1203 ,  C07K16/121 ,  C07K2317/76 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/56922 ,  G01N2333/205 ,  Y02A50/451

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：EP1698698A4

公开(公告)日：2008-07-09

申请号：EP04819936

申请日：2004-12-03

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO

IPC: C12Q1/68 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N15/31 ,  C12P21/02 ,  G01N33/50 ,  G01N33/569

CPC classification number: C07K14/205 ,  C12Q1/686 ,  C12Q1/689 ,  C12Q2600/16 ,  Y02A50/451

Abstract: The present inventors succeeded in cloning the CDT genes of C. coli and C. fetus, which were previously unknown, and in determining their sequences. In addition, the inventors also developed specific primers and primers common to the two species by comparing the CDTs of C. jejuni and C. fetus. Furthermore, the inventors demonstrated that these primers were applicable to multiplex PCR that simultaneously allows for the rapid and convenient determination of the presence of Campylobacter CDT and identification of species, and that they can also be used in PCR-RFLP-based typing.

公开(公告)号：CA2548381C

公开(公告)日：2015-12-01

申请号：CA2548381

申请日：2004-12-03

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO

IPC: C12N15/31 ,  C07H21/04 ,  C07K14/205 ,  C07K16/12 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/569

Abstract: The present inventors succeeded in cloning the CDT genes of C. coli and C. fetus, which were previously unknown, and in determining their sequences. In addition, the inventors also developed specific primers and primers common to the two species by comparing the CDTs of C. jejuni and C. fetus. Furthermore, the inventors demonstrated that these primers were applicable to multiplex PCR that simultaneously allows for the rapid and convenient determination of the presence of Campylobacter CDT and identification of species, and that they can also be used in PCR-RFLP-based typing.

公开(公告)号：AU2008292375A2

公开(公告)日：2010-04-29

申请号：AU2008292375

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO ,  YAMASAKI SHINJI

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：CA2718214A1

公开(公告)日：2009-03-05

申请号：CA2718214

申请日：2008-02-26

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO

IPC: C12Q1/68 ,  C12N15/09 ,  C12Q1/04

Abstract: Multiplex PCR primers that can amplify the cdt genes of C. jejuni, C. coli, and C. fetus in a bacterial species-specific manner were prepared. Multiplex PCR with the primers was assessed using Campylobacter bacteria, other cdt gene-positive bacteria, and representative bacteria responsible for enteric infection. As a result, the present inventors' multiplex PCR using cdtB amplification primers was proven to enable simultaneous detection of different Campylobacter bacteria with high specificity. The methods of the present invention can identify Campylobacter at the bacterial species level in a single manipulation even when domestic animals or humans are infected with different bacterial species of Campylobacter.

公开(公告)号：AU2008292946A1

公开(公告)日：2009-03-05

申请号：AU2008292946

申请日：2008-02-26

Applicant: FUSO PHARMACEUTICAL IND ,  UNIV OSAKA PREFECTURE

Inventor： ASAKURA MASAHIRO ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/09 ,  C12Q1/04

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C. hyointestinalis and polynucleotides encoding it, and novel methods for detection of C. hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni, C. coli, and C. fetus, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified as C. hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：AU2008292375B2

公开(公告)日：2014-07-03

申请号：AU2008292375

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO ,  SAMOSORUNSUK WORADA

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: Disclosed are: a cytolethal distending toxin (CDT) derived from

公开(公告)号：AU2004295596A2

公开(公告)日：2005-06-16

申请号：AU2004295596

申请日：2004-12-03

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO

IPC: C07K14/205 ,  C12Q1/68

Abstract: The present inventors succeeded in cloning the CDT genes of C. coli and C. fetus, which were previously unknown, and in determining their sequences. In addition, the inventors also developed specific primers and primers common to the two species by comparing the CDTs of C. jejuni and C. fetus. Furthermore, the inventors demonstrated that these primers were applicable to multiplex PCR that simultaneously allows for the rapid and convenient determination of the presence of Campylobacter CDT and identification of species, and that they can also be used in PCR-RFLP-based typing.

公开(公告)号：AU2015215750A1

公开(公告)日：2016-09-01

申请号：AU2015215750

申请日：2015-01-29

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIMOTO NORIHIKO ,  EDA SOUJI ,  ASAKURA MASAHIRO ,  ABE KANAKO ,  UEHARA HIROTSUGU ,  KAMEI KAZUMASA ,  UESAKA YOSHIHIKO ,  OKU YUICHI ,  SHIBAHARA YUSUKE

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09

Abstract: Through the present invention, a mask oligonucleotide is hybridized in regions on either side of a region in which an oligonucleotide probe is hybridized in a single-stranded region of a nucleic acid to be assayed, the region in which the probe is hybridized is thereby opened and the single-stranded region of the target nucleic acid is maintained in a stable state. By then subjecting the nucleic acid having such a single-stranded region to nucleic acid chromatography, the desired target nucleic acid is detected and quantified with high sensitivity by an extremely simple process.