公开(公告)号：EP3103884A4

公开(公告)日：2017-08-23

申请号：EP15746728

申请日：2015-01-29

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIMOTO NORIHIKO ,  EDA SOUJI ,  ASAKURA MASAHIRO ,  ABE KANAKO ,  UEHARA HIROTSUGU ,  KAMEI KAZUMASA ,  UESAKA YOSHIHIKO ,  OKU YUICHI ,  SHIBAHARA YUSUKE

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09

CPC classification number: C12Q1/6832 ,  C12Q2537/162 ,  C12Q2565/133 ,  C12Q2565/137

公开(公告)号：EP1721991A4

公开(公告)日：2007-10-17

申请号：EP05709892

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

CPC classification number: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q2531/113

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：CA2938817A1

公开(公告)日：2015-08-13

申请号：CA2938817

申请日：2015-01-29

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIMOTO NORIHIKO ,  EDA SOUJI ,  ASAKURA MASAHIRO ,  ABE KANAKO ,  UEHARA HIROTSUGU ,  KAMEI KAZUMASA ,  UESAKA YOSHIHIKO ,  OKU YUICHI ,  SHIBAHARA YUSUKE

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09

Abstract: Through the present invention, a mask oligonucleotide is hybridized in regions on either side of a region in which an oligonucleotide probe is hybridized in a single-stranded region of a nucleic acid to be assayed, the region in which the probe is hybridized is thereby opened and the single-stranded region of the target nucleic acid is maintained in a stable state. By then subjecting the nucleic acid having such a single-stranded region to nucleic acid chromatography, the desired target nucleic acid is detected and quantified with high sensitivity by an extremely simple process.

公开(公告)号：HK1101414A1

公开(公告)日：2007-10-18

申请号：HK07106180

申请日：2007-06-11

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMAZAKI SHINJI

IPC: C12N20060101 ,  C12N15/10 ,  C12Q20060101 ,  C12Q1/68 ,  G01N20060101 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：AU2015215750A1

公开(公告)日：2016-09-01

申请号：AU2015215750

申请日：2015-01-29

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIMOTO NORIHIKO ,  EDA SOUJI ,  ASAKURA MASAHIRO ,  ABE KANAKO ,  UEHARA HIROTSUGU ,  KAMEI KAZUMASA ,  UESAKA YOSHIHIKO ,  OKU YUICHI ,  SHIBAHARA YUSUKE

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09

Abstract: Through the present invention, a mask oligonucleotide is hybridized in regions on either side of a region in which an oligonucleotide probe is hybridized in a single-stranded region of a nucleic acid to be assayed, the region in which the probe is hybridized is thereby opened and the single-stranded region of the target nucleic acid is maintained in a stable state. By then subjecting the nucleic acid having such a single-stranded region to nucleic acid chromatography, the desired target nucleic acid is detected and quantified with high sensitivity by an extremely simple process.

公开(公告)号：AU2005210362B2

公开(公告)日：2008-05-29

申请号：AU2005210362

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAZAKI SHINJI ,  YAMAMOTO SEIJI ,  EDA SOUJI

IPC: G01N33/50 ,  C12N15/10 ,  C12Q1/68

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：CA2555346A1

公开(公告)日：2005-08-18

申请号：CA2555346

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAMAMOTO SEIJI ,  MATSUHISA AKIO ,  EDA SOUJI ,  YAMAZAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detecti on kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucle ic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：CA2555346C

公开(公告)日：2015-04-21

申请号：CA2555346

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：AU2005210362A1

公开(公告)日：2005-08-18

申请号：AU2005210362

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAZAKI SHINJI ,  YAMAMOTO SEIJI ,  EDA SOUJI

IPC: G01N33/50 ,  C12N15/10 ,  C12Q1/68

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.