公开(公告)号：EP1403369A4

公开(公告)日：2005-06-15

申请号：EP02778910

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  KESHI HIROYUKI ,  ABE KANAKO ,  SUGIMOTO NORIHIKO ,  UEYAMA HIROSHI ,  EDA SOJI ,  UEHARA HIROTSUGU ,  IWAMI TAKAHISA ,  YAMAMOTO SEIJI ,  ARAKI HIROMASA

IPC: C12Q1/68 ,  G01N33/50 ,  C12N15/09 ,  C12N5/06 ,  C12N5/10 ,  G01N33/53

CPC classification number: G01N33/5047 ,  C12Q1/6841 ,  C12Q1/689 ,  G01N33/5005

Abstract: Phagocytes having taken a foreign microorganism which are prepared by bringing phagocytes into contact with the microorganism and then isolating the phagocytes; a process for producing the same; and a method and a kit with the use of the same. An experimental model whereby the phagocytosis function of phagocytes can be evaluated in vitro .

公开(公告)号：EP1721991A4

公开(公告)日：2007-10-17

申请号：EP05709892

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

CPC classification number: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q2531/113

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：HK1101414A1

公开(公告)日：2007-10-18

申请号：HK07106180

申请日：2007-06-11

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMAZAKI SHINJI

IPC: C12N20060101 ,  C12N15/10 ,  C12Q20060101 ,  C12Q1/68 ,  G01N20060101 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：AU2005210362B2

公开(公告)日：2008-05-29

申请号：AU2005210362

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAZAKI SHINJI ,  YAMAMOTO SEIJI ,  EDA SOUJI

IPC: G01N33/50 ,  C12N15/10 ,  C12Q1/68

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：CA2555346A1

公开(公告)日：2005-08-18

申请号：CA2555346

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAMAMOTO SEIJI ,  MATSUHISA AKIO ,  EDA SOUJI ,  YAMAZAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detecti on kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucle ic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：CA2447775A1

公开(公告)日：2002-12-19

申请号：CA2447775

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  ABE KANAKO ,  KESHI HIROYUKI ,  SUGIMOTO NORIHIKO ,  ARAKI HIROMASA ,  YAMAMOTO SEIJI ,  OHNO TSUNEYA ,  UEYAMA HIROSHI ,  IWAMI TAKAHISA ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12Q1/68 ,  G01N33/50 ,  C12N15/09 ,  C12N5/06 ,  C12N5/10 ,  G01N33/53

Abstract: Phagocytes having taken a foreign microorganism which are prepared by bringi ng phagocytes into contact with the microorganism and then isolating the phagocytes; a process for producing the same; and a method and a kit with th e use of the same. An experimental model whereby the phagocytosis function of phagocytes can be evaluated in vitro.

公开(公告)号：CA2555346C

公开(公告)日：2015-04-21

申请号：CA2555346

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：AU2005210362A1

公开(公告)日：2005-08-18

申请号：AU2005210362

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAZAKI SHINJI ,  YAMAMOTO SEIJI ,  EDA SOUJI

IPC: G01N33/50 ,  C12N15/10 ,  C12Q1/68

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.