公开(公告)号：JP2008070366A

公开(公告)日：2008-03-27

申请号：JP2007234331

申请日：2007-09-10

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： SPINKE JUERGEN ,  THIELE MARCEL ,  SCHAEFFLER JUERGEN ,  NUFER ANDREAS

IPC: G01N33/557 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/74 ,  G01N33/5306 ,  G01N33/54386 ,  G01N33/557

Abstract: PROBLEM TO BE SOLVED: To provide a method and device for quantitatively determining an analyte in a sample. SOLUTION: Based on generated calibration graphs, it is examined whether a signal generated by reaction between an analyte-specific substance and the analyte in the sample enables quantitative determination of the analyte with a desired accuracy. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供用于定量测定样品中的分析物的方法和装置。 解决方案：基于生成的校准图，检查由分析物特异性物质与样品中的分析物之间的反应产生的信号是否能够以期望的精度定量测定分析物。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2004264296A

公开(公告)日：2004-09-24

申请号：JP2004025781

申请日：2004-02-02

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： HORN CARINA ,  HOENES JOACHIM ,  SPINKE JUERGEN

IPC: G01N31/22 ,  C12Q1/26 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N2021/7786 ,  Y10T436/104998 ,  Y10T436/106664 ,  Y10T436/144444

Abstract: PROBLEM TO BE SOLVED: To provide an oxidation/reduction-active compound as a detection reagent for measuring fluorescence in analyte, for solving defects in prior art at least partially. SOLUTION: A method for detecting analyte by oxidation/reduction reaction and fluorescence measurement brings a sample, containing analyte into contact with the detection reagent containing a compound expressed by as a fluorescent oxidation reduction indicator. NBD-amine-N-oxide, or its derivative is obtained as a redox indicator. In this manner, NBD-amine formed by reduction features high fluorescence and can be extremely easily excited by blue light irradiation, thus solving nonconformities, where the number of these known as a compound for use, for example, especially strong blue and read LEDs is not sufficient, since an excitation light source to be used is limited mainly to UV and green regions in known fluorescence detection reagents. COPYRIGHT: (C)2004,JPO&NCIPI

公开(公告)号：JP2004258021A

公开(公告)日：2004-09-16

申请号：JP2004017685

申请日：2004-01-26

Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

Inventor： HORN CARINA ,  JOSEL HANS-PETER ,  SPINKE JUERGEN ,  HERRMANN RUPERT ,  HEINDL DIETER

IPC: G01N31/22 ,  C12Q1/26 ,  G01N21/64 ,  G01N21/78 ,  G01N33/58 ,  G01N33/66

CPC classification number: G01N21/6428 ,  G01N2021/6432 ,  Y10S436/80 ,  Y10S436/904

Abstract: PROBLEM TO BE SOLVED: To provide a method and a reagent kit n for fluorometry of an analyte, and a novel oxidation-reduction active compound serving as a fluorescent reagent for the analyte. SOLUTION: In this method for detecting the analyte by an oxidation-reduction reaction and the fluorometry, a sample containing the analyte is brought into contact with a detecting reagent containing a compound expressed by the following general formula (1): Q-F(Q is a quencher group, and F is a fluorophore group) as a fluorescent oxidation-reduction indicator. Examples favorable as the reducible quencher group are quinones, aromatic nitroso compounds such as nitrosoanilines and other nitrosobenzene derivatives, and N-oxides. COPYRIGHT: (C)2004,JPO&NCIPI

Abstract translation: 要解决的问题：提供用于分析物的荧光测定的方法和试剂盒n，以及用作分析物的荧光试剂的新型氧化还原活性化合物。 解决方案：在通过氧化还原反应和荧光测定法检测分析物的方法中，使含有分析物的样品与含有由以下通式（1）表示的化合物的检测试剂接触：QF（ Q是猝灭基团，F是荧光团）作为荧光氧化还原指示剂。 可还原的猝灭剂组是醌类，亚硝基苯胺和其它亚硝基苯衍生物等芳香族亚硝基化合物，N-氧化物等。 版权所有（C）2004，JPO＆NCIPI

公开(公告)号：WO2005111607A3

公开(公告)日：2006-02-02

申请号：PCT/EP2005005016

申请日：2005-05-10

Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  SPINKE JUERGEN ,  SCHAEFFLER JUERGEN ,  BODENBACH LUDGER

Inventor： SPINKE JUERGEN ,  SCHAEFFLER JUERGEN ,  BODENBACH LUDGER

IPC: G01N33/538 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/54386 ,  G01N33/538

Abstract: The invention relates to a method for increasing the dynamic measuring range of test elements, especially immunological test elements, especially optically readable immunological chromatography strips, that are based on specific bonding reactions. The invention allows to displace the dynamic test range of especially immunological test elements that are based on specific binding reactions towards higher analyte concentrations without forfeiting sensitivity. For this purpose, at least two zones in or on the test element are provided and contain reagents that produce differently strong detectable signals due to different affinities to the analyte (for example in the case of antibodies that have different affinity to the analyte) or due to different principles of interaction with the analyte or with other reagents involved in the detection of the analyte (for example antibodies directed to the analyte in one zone and analyte or analyte analog in a different zone). The signals in the at least two zones are used for evaluation of the analyte concentration/signal strength correlation and used for determining the analyte by way of a suitable method (correlation).

Abstract translation: 本发明涉及一种用于提高基于特异性结合反应，特别是免疫学测试元件，特别是光学可评价免疫层析试验的动态测量范围。 本发明使得能够移动基于特定结合反应，在向更高的分析物浓度特定的免疫学测试元件测量的动态范围，而不必接受鉴于检测限的损失。 为了这个目的，本发明提出了在或在测试元件上提供至少两个区域，所述试剂包含所述分析物由于不同的亲和力（例如，在针对所述分析物的不同亲和力的抗体的情况下）或由于与分析物或与其它的分析物相互作用的不同的原理 参与的试剂（例如，抗体针对在一个区中的分析物，和分析物或分析物在不同的区）产生不同强度的检测的信号。 为了评估的分析物浓度的信号强度的关系，该信号在至少两个区，并通过用于分析物测定的合适方法（相关性）使用。

公开(公告)号：BRPI0405215B1

公开(公告)日：2016-08-02

申请号：BRPI0405215

申请日：2004-11-25

Applicant: HOFFMANN LA ROCHE

Inventor： NICHTL ALFONS ,  GALLUSSER ANDREAS ,  BORGYA ANNELIESE ,  SPINKE JUERGEN ,  HALLERMAYER KLAUS ,  GROL MICHAEL ,  KLEMT VOLKER

IPC: G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/553 ,  G01N33/577 ,  G01N33/68 ,  G01N33/74

Abstract: "teste analítico em sanduíche para determinar nt-probnp". a presente invenção refere-se a testes imunológicos rápidos para determinar nt-probnp, que usam combinações especiais de anticorpos. o teste rápido, de acordo com a invenção, pode estar presente com vantagens como uma tira de teste imunocromatográfico.

公开(公告)号：AT551598T

公开(公告)日：2012-04-15

申请号：AT04001727

申请日：2004-01-27

Applicant: HOFFMANN LA ROCHE

Inventor： HORN CARINA DR ,  JOSEL HANS-PETER ,  SPINKE JUERGEN ,  HERRMANN RUPERT ,  HEINDL DIETER

IPC: G01N21/64 ,  G01N31/22 ,  C12Q1/26 ,  G01N21/77 ,  G01N21/78 ,  G01N33/58 ,  G01N33/66

Abstract: Detecting an analyte (A) by a redox reaction and fluorimetric measurement, comprises that the (A)-containing sample is treated with a fluorimetric redox indicator (I) that comprises a quencher group (Q) linked to a fluorophore (F). An independent claim is also included for a reagent for the process that comprises (I) where at least one of Q and F is oxidizable or reducible, and the fluorescence can be altered, depending on oxidation or reduction.

公开(公告)号：AT487946T

公开(公告)日：2010-11-15

申请号：AT04027831

申请日：2004-11-24

Applicant: HOFFMANN LA ROCHE

Inventor： SPINKE JUERGEN ,  NICHTL ALFONS ,  KLEMT VOLKER ,  HALLERMAYER KLAUS ,  GROL MICHAEL ,  BORGYA ANNELIESE ,  GALLUSSER ANDREAS

IPC: G01N33/53 ,  G01N33/577 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74

Abstract: An immunological sandwich test determines the presence of NT-proBNP and uses two or more antibodies on the NT-proBNP. An immunological sandwich test determines the presence of NT-proBNP and uses two or more antibodies on the NT-proBNP. At least one antibody is a MAK. One of these antibodies is effective against part of the NT-proBNP epitope incorporating amino-acids 38 to 50. One of these antibodies is directed at parts of the NT-proBNP epitope with amino-acids 1 to 37, or 43 to 76. The epitopes recognised by the anti-bodies have a small overlap range.

公开(公告)号：HK1118608A1

公开(公告)日：2009-02-13

申请号：HK08110069

申请日：2008-09-10

Applicant: HOFFMANN LA ROCHE

Inventor： SPINKE JUERGEN ,  THIELE MARCEL ,  SCHAEFFLER JUERGEN ,  NUFER ANDREAS

IPC: G01N20060101

公开(公告)号：CA2456342C

公开(公告)日：2011-06-21

申请号：CA2456342

申请日：2004-01-28

Applicant: HOFFMANN LA ROCHE

Inventor： HORN CARINA ,  HOENES JOACHIM ,  SPINKE JUERGEN

IPC: G01N31/22 ,  G01N33/52 ,  C12Q1/26 ,  G01N21/64 ,  G01N33/50 ,  G01N33/66

Abstract: The invention concerns methods and reagent kits for the fluorimetric determination of analytes.

公开(公告)号：CA2566040C

公开(公告)日：2010-07-20

申请号：CA2566040

申请日：2005-05-10

Applicant: HOFFMANN LA ROCHE

Inventor： SPINKE JUERGEN ,  SCHAEFFLER JUERGEN ,  BODENBACH LUDGER

IPC: G01N33/538 ,  G01N33/53 ,  G01N33/543

Abstract: The invention relates to a method for increasing the dynamic measuring range of test elements, especially immunological test elements, especially optically readable immunological chromatography strips, that are based on specific bonding reactions. The invention allows to displace the dynamic test range of especially immunological test elements that are based on specific binding reactions towards higher analyte concentrations without forfeiting sensitivity. For this purpose, at least two zones in or on the test element are provided and contain reagents that produce differently strong detectable signals due to different affinities to the analyte (for example in the case of antibodies that have different affinity to the analyte) or due to different principles of interaction with the analyte or with other reagents involved in the detection of the analyte (for example antibodies directed to the analyte in one zone and analyte or analyte analog in a different zone). The signals in the at least two zones are used for evaluation of the analyte concentration/signal strength correlation and used for determining the analyte by way of a suitable method (correlation).