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1.发明专利Growth medium for clostridium histolyticum without ingredient of mammalian source 有权不含马鞭草源成分的胶原蛋白的生长培养基
公开(公告)号：JP2009297028A
公开(公告)日：2009-12-24
申请号：JP2009139667
申请日：2009-06-10
Applicant: F Hoffmann-La Roche Ag , エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト
Inventor： HOELKE WERNER , HOFFMANN ARTUR , MARX THOMAS , SONN KIRSTEN , SUPPMANN BERNHARD , THALHOFER JOHANN-PETER
Abstract: PROBLEM TO BE SOLVED: To provide an improved medium for culturing Clostridium histolyticum and culture supernatants for biotechnologically producing collagenase enzymes.
SOLUTION: The culture media contain water, fish gelatin and peptones derived from a non-mammalian source. The media preferably contain peptones derived from plants as a non-mammalian source. The media also may contain two or more different peptones. Disclosed are sterilized compositions comprising the media, a culture supernatant containing the collagenase of Clostridium histolyticum, a method for producing the culture supernatant, and a method for producing the collagenase.
COPYRIGHT: (C)2010,JPO&INPITAbstract translation: 要解决的问题：提供用于培养溶组织梭菌和用于生物技术生产胶原酶的培养上清液的改进培养基。 解决方案：培养基含有源自非哺乳动物来源的水，鱼明胶和胨。 培养基优选含有源自植物的胨作为非哺乳动物来源。 媒体也可能含有两种或更多种不同的蛋白胨。 公开了包含培养基的灭菌组合物，含有溶组织梭菌胶原酶的培养上清液，培养上清液的制备方法和胶原酶的制备方法。 版权所有（C）2010，JPO＆INPIT
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2.发明专利
公开(公告)号：JP2003304889A
公开(公告)日：2003-10-28
申请号：JP2003099615
申请日：2003-04-02
Applicant: F Hoffmann La Roche Ag , エフ.ホフマン−ラ ロシュ アーゲー
Inventor： MUELLER RAINER , PAJATSCH MARKUS , CURDT INGO , SOBEK HARALD DR , SCHMIDT MANFRED , SUPPMANN BERNHARD , SONN KIRSTEN , SCHNEIDINGER BERND
CPC classification number: C12N9/1264 , C12Y207/07031 , G01N2510/00
Abstract: PROBLEM TO BE SOLVED: To provide a terminal deoxynucleotidyl transferase having uniform state with sufficient enzymatic activity. SOLUTION: The transferase is a truncated TdT derivative wherein at most 161 amino acids at the N-terminal are truncated and exhibiting enzymatic activity in a solution containing Co 2+ ion corresponding to 20-30 times the enzymatic activity of natural terminal deoxynucleotidyl transferase (TdT). COPYRIGHT: (C)2004,JPO
Abstract translation: 要解决的问题：提供具有均匀状态的末端脱氧核苷酸转移酶，具有足够的酶活性。 解决方案：转移酶是截短的TdT衍生物，其N-末端的至多161个氨基酸被截短并且在含有对应于20-30倍的Co
2 + 离子的溶液中表现出酶活性 天然末端脱氧核苷酸转移酶（TdT）的酶活性。 版权所有（C）2004，JPO -
3.发明专利
公开(公告)号：ES2558430T3
公开(公告)日：2016-02-04
申请号：ES09007502
申请日：2009-06-06
Applicant: HOFFMANN LA ROCHE
Inventor： HOELKE WERNER , HOFFMANN ARTUR , MARX THOMAS , SONN KIRSTEN , SUPPMANN BERNHARD , THALHOFER JOHANN-PETER
Abstract: Un medio líquido para el cultivo de Clostridium histolyticum, comprendiendo el medio agua, una gelatina de pescado y una peptona vegetal, seleccionándose la fuente de la peptona del grupo que consiste en soja, haba, guisante, patata, y una mezcla de los mismos, en el cual la concentración de agregado de la peptona o peptonas vegetales en el medio es entre aproximadamente el 2 % y aproximadamente el 10 % en peso por volumen, y por lo cual en el medio la concentración de gelatina de pescado es entre aproximadamente el 2 % y aproximadamente el 10 %, indicando el porcentaje peso por volumen cuando al gelatina de pescado aislada es materia seca y volumen por volumen cuando la gelatina de pescado aislada es materia líquida.
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公开(公告)号：CA2668353C
公开(公告)日：2017-11-07
申请号：CA2668353
申请日：2009-06-08
Applicant: HOFFMANN LA ROCHE
Inventor： WERNER HOELKE , HOFFMANN ARTUR , MARX THOMAS , SUPPMANN BERNHARD , THALHOFER JOHANN-PETER , SONN KIRSTEN
Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.
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公开(公告)号：AT317898T
公开(公告)日：2006-03-15
申请号：AT03006965
申请日：2003-03-27
Applicant: HOFFMANN LA ROCHE
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Patent Agency Ranking
公开(公告)号：HK1139705A1
公开(公告)日：2010-09-24
申请号：HK10105863
申请日：2010-06-11
Applicant: HOFFMANN LA ROCHE
Inventor： HOELKE WERNER , HOFFMANN ARTUR , MARX THOMAS , SONN KIRSTEN , SUPPMANN BERNHARD , THALHOFER JOHANN-PETER
IPC: C12N20060101 , C12R20060101
Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.
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公开(公告)号：CA2423887C
公开(公告)日：2008-01-22
申请号：CA2423887
申请日：2003-04-01
Applicant: HOFFMANN LA ROCHE
Inventor： PAJATSCH MARKUS , MUELLER RAINER , SOBEK HARALD , CURDT INGO , SONN KIRSTEN , SCHMIDT MANFRED , SUPPMANN BERNHARD , SCHNEIDINGER BERND
IPC: C12N9/12 , C12N15/09 , C12N9/10 , C12N15/54 , C12N15/63 , C12P19/34 , C12P21/02 , C12Q1/48 , G01N33/573
Abstract: Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recombinant production and use.
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公开(公告)号：SG158025A1
公开(公告)日：2010-01-29
申请号：SG2009039108
申请日：2009-06-08
Applicant: HOFFMANN LA ROCHE
Inventor： HOELKE WERNER , HOFFMANN ARTUR , MARX THOMAS , SONN KIRSTEN , SUPPMANN BERNHARD , THALHOFER JOHANN-PETER
Abstract: Growth medium for C. histolyticum without ingredients of mamalian sources The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one 5 or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.
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公开(公告)号：CA2668353A1
公开(公告)日：2009-12-11
申请号：CA2668353
申请日：2009-06-08
Applicant: HOFFMANN LA ROCHE
Inventor： SUPPMANN BERNHARD , HOFFMANN ARTUR , WERNER HOELKE , THALHOFER JOHANN-PETER , MARX THOMAS , SONN KIRSTEN
Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.
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公开(公告)号：CA2423887A1
公开(公告)日：2003-10-05
申请号：CA2423887
申请日：2003-04-01
Applicant: HOFFMANN LA ROCHE
Inventor： PAJATSCH MARKUS , CURDT INGO , SCHNEIDINGER BERND , SOBEK HARALD , SCHMIDT MANDFRED , SUPPMANN BERNHARD , MUELLER RAINER , SONN KIRSTEN
IPC: C12N15/09 , C12N9/12 , C12N15/54 , C12Q1/48 , C12N9/10 , C07K19/00 , C12N15/62 , C12N15/63 , C12P21/02 , G01N33/573
Abstract: Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recombinant production and use.
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