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公开(公告)号：WO2011106634A3
公开(公告)日：2012-05-03
申请号：PCT/US2011026228
申请日：2011-02-25
Applicant: LIFE TECHNOLOGIES CORP , DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
CPC classification number: C12N9/1252
Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的修饰的核酸结合蛋白的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰的修饰蛋白以实现期望的pKa值的方法。 此外，本发明提供了在离子生成反应中使用这些修饰蛋白的方法，如基于离子的核酸测序反应。
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公开(公告)号：WO2011106770A3
公开(公告)日：2012-04-05
申请号：PCT/US2011026468
申请日：2011-02-28
Applicant: LIFE TECHNOLOGIES CORP , DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
CPC classification number: C12N9/1252
Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
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公开(公告)号：WO2011106766A3
公开(公告)日：2012-04-26
申请号：PCT/US2011026450
申请日：2011-02-28
Applicant: LIFE TECHNOLOGIES CORP , DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
CPC classification number: C12N9/1252
Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
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公开(公告)号：SG11201406717RA
公开(公告)日：2014-11-27
申请号：SG11201406717R
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORP
Inventor： LI CHIEH-YUAN , RUFF DAVID , CHEN SHIAW-MIN , O'NEIL JENNIFER , KASINSKAS RACHEL , ROTHBERG JONATHAN
IPC: C12Q1/68
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公开(公告)号：LT3040424T
公开(公告)日：2018-05-10
申请号：LT15192848
申请日：2011-02-28
Applicant: LIFE TECHNOLOGIES CORP
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
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Patent Agency Ranking
公开(公告)号：AU2011220536B2
公开(公告)日：2012-05-24
申请号：AU2011220536
申请日：2011-02-25
Applicant: LIFE TECHNOLOGIES CORP
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
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公开(公告)号：SG10201802883UA
公开(公告)日：2018-05-30
申请号：SG10201802883U
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORP
Inventor： LI CHIEH-YUAN , RUFF DAVID , CHEN SHIAW-MIN , O'NEIL JENNIFER , KASINSKAS RACHEL , ROTHBERG JONATHAN
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公开(公告)号：AU2012216424B2
公开(公告)日：2013-03-07
申请号：AU2012216424
申请日：2012-08-24
Applicant: LIFE TECHNOLOGIES CORP
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Abstract: The present invention provides a method of detecting a nucleotide incorporation, comprising: (a) performing a nucleotide incorporation using a modified polymerase and generating one or more by-products of the nucleotide incorporation, where the modified polymerase has a reduced buffering capacity relative to a corresponding unmodified polymerase; and (b) detecting the presence of the one or more by-products of the nucleotide incorporation, thereby detecting the nucleotide incorporation.
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公开(公告)号：AU2012216424A1
公开(公告)日：2012-09-13
申请号：AU2012216424
申请日：2012-08-24
Applicant: LIFE TECHNOLOGIES CORP
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
Abstract: The present invention provides a method of detecting a nucleotide incorporation, comprising: (a) performing a nucleotide incorporation using a modified polymerase and generating one or more by-products of the nucleotide incorporation, where the modified polymerase has a reduced buffering capacity relative to a corresponding unmodified polymerase; and (b) detecting the presence of the one or more by-products of the nucleotide incorporation, thereby detecting the nucleotide incorporation.
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公开(公告)号：EP2539473A4
公开(公告)日：2013-03-13
申请号：EP11748228
申请日：2011-02-28
Applicant: LIFE TECHNOLOGIES CORP
Inventor： DAVIDSON JOHN F , HINZ WOLFGANG , ROTHBERG JONATHAN , WHITAKER RICHARD
CPC classification number: C12N9/1252
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