公开(公告)号：WO2011026141A3

公开(公告)日：2011-04-21

申请号：PCT/US2010047402

申请日：2010-08-31

Applicant: LIFE TECHNOLOGIES CORP ,  COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

Inventor： COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

IPC: G02B7/00 ,  G02B21/00

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast- indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast- indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelength are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括快速分度滤光轮，其具有能够快速旋转进入激发源（例如，弧光灯，激光器）和与激光源（例如，弧光灯，激光器）连通的多个激发和发射滤光片。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以配置为考虑快速启动和停止快速分度滤光轮以及其他来源产生振动的振动。 本文还提供了用于快速成像多个波长的样品的各种方法。

公开(公告)号：WO2011026128A2

公开(公告)日：2011-03-03

申请号：PCT/US2010047377

申请日：2010-08-31

Applicant: LIFE TECHNOLOGIES CORP ,  HIRANO KIRK ,  ANDERSEN MARK ,  GONG JIAN ,  WOO SAM ,  COX DAVID ,  YANG JOON MO ,  YUE MIN ,  SHARIATI MARYAM ,  BRIDGHAM JOHN ,  LIU DAVID

Inventor： HIRANO KIRK ,  ANDERSEN MARK ,  GONG JIAN ,  WOO SAM ,  COX DAVID ,  YANG JOON MO ,  YUE MIN ,  SHARIATI MARYAM ,  BRIDGHAM JOHN ,  LIU DAVID

IPC: B01J19/00 ,  B01L3/00 ,  G01N35/10

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.

Abstract translation: 提供了各种流通池配置和系统以及制造和使用它们的方法。 流通池，系统和使用方法可用于进行测序反应和下一代测序方法。

公开(公告)号：EP1885839A4

公开(公告)日：2015-05-27

申请号：EP06751668

申请日：2006-04-26

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YUE MIN ,  LIU DAVID M ,  CHIANG YUH-MIN ,  YANG JOON MO ,  LEHTO DENNIS ,  VANN CHARLES S ,  BEARD NIGEL P ,  HARDING IAN A ,  VAN CAMP JOHN ,  DROMARETSKY ALEXANDER ,  ERMAKOV SERGEY V ,  OLDHAM MARK F ,  ROY JOY ,  SHARIATI MARYAM ,  ULMANELLA UMBERTO

IPC: B01L3/00 ,  C12Q1/68 ,  G01N35/02

CPC classification number: C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502723 ,  B01L3/50853 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0694 ,  G01N35/028 ,  Y10T436/2575

Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于对进入基底的生物样品进行核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品不会从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。