Abstract:
A method for delivering reagent during a thermal cycling reaction may include placing a delivery device containing a reagent proximate to a reaction site, initiating a first stage of the reaction, wherein the first stage includes a period of an elevated temperature, and after completing the first stage of the reaction, dispensing the reagent from the delivery device to the reaction site, wherein the dispensing includes automatically actuating the delivery device to deliver the reagent.
Abstract:
Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.
Abstract:
Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.