公开(公告)号：WO2011127454A2

公开(公告)日：2011-10-13

申请号：PCT/US2011031855

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP ,  JANAWAY GORDON ,  CHAN EVELYN WING-SIM

Inventor： JANAWAY GORDON ,  CHAN EVELYN WING-SIM

IPC: G06F19/00 ,  G06F3/00

CPC classification number: G06F19/18 ,  G06F3/0482 ,  G06F3/0485 ,  G06F19/26

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

Abstract translation: 系统和方法用于显示从qPCR仪器获得的数据。 用第一标记探针和第二标记探针探测两个或更多个样品中的每一个。 从qPCR仪器以第一周期数接收第一数据集，其中包括针对每个样本的第一标记探针强度和第二标记探针强度。 第二数据集以第二周期数被接收，其包括针对每个样本的第一标记探针强度和第二标记探针强度。 使用第一数据集创建第一标记探针强度作为第二标记探针强度的函数的第一图。 使用第二数据集创建第二标记探针强度作为第二标记探针强度的函数的第二图。 显示第一个绘图和第二个绘图以响应用户定义的输入，以提供对基因分型数据的动态和实时分析。

公开(公告)号：SG11201401917QA

公开(公告)日：2014-09-26

申请号：SG11201401917Q

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： JANAWAY GORDON ,  ALIMINATI MANJULA ,  WU RUOYUN ,  FORTESCUE DAVID ,  SHEN MING

IPC: C12Q1/68 ,  G16B45/00

Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting of emission data from a planiality of samples, each included in a sample region of a plurality of sample regions, at a first time amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data, A dPCR result is generated based on the positive amplification determinations for the plurality of samples.

公开(公告)号：SG11201502797PA

公开(公告)日：2015-05-28

申请号：SG11201502797P

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MAJUMDAR NIVEDITA SUMI ,  WESSEL THOMAS ,  WOO DAVID C ,  SIKORA MARCIN ,  JANAWAY GORDON ,  RAIZADA SHWETA ,  LANKESTER JOANNA ,  MARKS JEFFREY ,  WESSEL DANIEL

IPC: G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

公开(公告)号：AU2013315167A1

公开(公告)日：2015-04-30

申请号：AU2013315167

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MAJUMDAR NIVEDITA SUMI ,  WESSEL THOMAS ,  WOO DAVID C ,  SIKORA MARCIN ,  JANAWAY GORDON ,  RAIZADA SHWETA ,  LANKESTER JOANNA ,  MARKS JEFFREY ,  WESSEL DANIEL

IPC: G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

公开(公告)号：SG184480A1

公开(公告)日：2012-11-29

申请号：SG2012074423

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： JANAWAY GORDON ,  CHAN EVELYN WING-SIM

IPC: G16B45/00

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

公开(公告)号：SG10201604446PA

公开(公告)日：2016-07-28

申请号：SG10201604446P

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： JANAWAY GORDON ,  ALIMINATI MANJULA ,  WU RUOYUN ,  FORTESCUE DAVID ,  SHEN MING

IPC: G16B45/00

Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting of emission data from a planiality of samples, each included in a sample region of a plurality of sample regions, at a first time amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data, A dPCR result is generated based on the positive amplification determinations for the plurality of samples.

公开(公告)号：IN3407DEN2014A

公开(公告)日：2015-06-05

申请号：IN3407DEN2014

申请日：2014-04-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： JANAWAY GORDON ,  ALIMINATI MANJULA ,  WU RUOYUN ,  FORTESCUE DAVID ,  SHEN MING

IPC: C12Q1/68 ,  G16B45/00

Abstract: A computer implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples each included in a sample region of a plurality of sample regions at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.

公开(公告)号：EP2556458A4

公开(公告)日：2016-03-16

申请号：EP11766848

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： JANAWAY GORDON ,  CHAN EVELYN WING-SIM

IPC: G06F19/26 ,  G06F19/18

CPC classification number: G06F19/18 ,  G06F3/0482 ,  G06F3/0485 ,  G06F19/26

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

Abstract translation: 系统和方法用于显示从qPCR仪器获得的数据。 用第一标记探针和第二标记探针探测两个或更多个样品中的每一个。 从qPCR仪器以第一周期数接收第一数据集，其包括针对每个样本的第一标记探针强度和第二标记探针强度。 第二数据集以第二周期数被接收，其包括针对每个样本的第一标记探针强度和第二标记探针强度。 使用第一数据集创建第一标记探针强度作为第二标记探针强度的函数的第一图。 使用第二数据集创建第二标记探针强度作为第二标记探针强度的函数的第二图。 显示第一个图和第二个图，以响应用户定义的输入，以提供对基因分型数据的动态和实时分析。