公开(公告)号：WO2012003412A2

公开(公告)日：2012-01-05

申请号：PCT/US2011042723

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORP ,  BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  Y02A50/451

Abstract: The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

Abstract translation: 本申请描述了使用可诱导RNA快速检测和鉴定各种微生物的组合物，方法和试剂盒。 描述了通过使用诱导剂在靶基因诱导后检测靶基因的诱导型RNA的表达来快速检测微生物的方法。 一些实施方案描述了用于快速检测微生物的方法和工作流程，例如但不限于沙门氏菌属，李斯特氏菌属（Listeria spp）。 和弧菌属（Vibrio spp。 组合物和试剂盒包含对对一种或多种RNA诱导剂有响应的微生物（或基因片段）基因的引物扩增具有杂交特异性的引物核酸序列。 在一些实施方案中，试剂盒和组合物还包含对对RNA诱导剂或其片段有响应的基因具有杂交特异性的探针核酸序列。

公开(公告)号：WO2011053871A3

公开(公告)日：2011-09-29

申请号：PCT/US2010054867

申请日：2010-10-29

Applicant: LIFE TECHNOLOGIES CORP ,  PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/48

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物，其中扩增产物的存在表示支原体 在样品中。

公开(公告)号：AU2021224298A1

公开(公告)日：2022-09-22

申请号：AU2021224298

申请日：2021-02-18

Applicant: LIFE TECHNOLOGIES CORP

Inventor： LI KELLY ,  PAGANI IOANNA ,  BRZOSKA PIUS ,  LI JISHENG ,  WANG CHUNLING ,  HAYASHIBARA KATHLEEN ,  TANNER MICHAEL ,  JI HONG ,  VARMA KAMINI ,  HU FANGQI ,  TEBBS ROBERT

IPC: C12Q1/70

Abstract: Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.

公开(公告)号：DK2902506T3

公开(公告)日：2016-12-05

申请号：DK15154931

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORP

Inventor： PETRAUSKENE OLGA ,  CUMMINGS CRAIG ,  VATTA PAOLO ,  TEBBS ROBERT ,  BALACHANDRAN PRIYA ,  ZODER PATRICK ,  WONG LILY

IPC: C12Q1/68