公开(公告)号：WO2011050340A1

公开(公告)日：2011-04-28

申请号：PCT/US2010/053873

申请日：2010-10-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  SIKORA, Marcin ,  BLANCHARD, Alan

Inventor： SIKORA, Marcin ,  BLANCHARD, Alan

IPC: C12Q1/68

CPC classification number: G06F19/20 ,  C12Q1/6869 ,  C12Q1/6874 ,  G06F19/10 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2521/501

Abstract: Disclosed are systems and methods for polynucleotide sequencing where detection and correction of base calling errors can be achieved without reliance on a reference sequence. In certain embodiments, redundant information, which may be provided by additional labels, can be introduced during measurement so as to allow such detection of errors. Such redundant information and measurements can be facilitated by encoding of nucleotide sequence being measured. Various examples of such encoding, redundancy introduction, and decoding are provided.

Abstract translation: 公开了用于多核苷酸测序的系统和方法，其中可以在不依赖参考序列的情况下实现基本呼叫错误的检测和校正。 在某些实施例中，可以在测量期间引入可由附加标签提供的冗余信息，以允许这种错误的检测。 可以通过编码被测量的核苷酸序列来促进这种冗余信息和测量。 提供了这种编码，冗余引入和解码的各种示例。

公开(公告)号：WO2013119956A1

公开(公告)日：2013-08-15

申请号：PCT/US2013/025352

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基 的非水溶剂。 底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

公开(公告)号：WO2012061818A1

公开(公告)日：2012-05-10

申请号：PCT/US2011/059607

申请日：2011-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BOEGE, Steven ,  BLANCHARD, Alan ,  BALL, James ,  FOSTER, Evan ,  BRIDGHAM, John

Inventor： BOEGE, Steven ,  BLANCHARD, Alan ,  BALL, James ,  FOSTER, Evan ,  BRIDGHAM, John

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2021/056

Abstract: Flowcell designs that are suitable for large scale gene sequencing analyses are disclosed. Different layouts of flowcells are presented. Methods of functionalizing the surface where DNA samples are placed for enhanced attachment are discussed. Special treatments of the functionalized surface to achieve desired optical characteristics are also featured.

Abstract translation: 公开了适用于大规模基因测序分析的流通池设计。 提出了流通池的不同布局。 讨论了放置DNA样品以增强附着物的表面官能化方法。 还具有功能化表面的特殊处理以实现期望的光学特性。

公开(公告)号：WO2011106460A2

公开(公告)日：2011-09-01

申请号：PCT/US2011/025964

申请日：2011-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION ,  MCKERNAN, Kevin ,  BLANCHARD, Alan ,  ZON, Gerald ,  LAO, Kai ,  STRAUS, Neil ,  SPIER, Eugene ,  CHEN, Caifu

Inventor： MCKERNAN, Kevin ,  BLANCHARD, Alan ,  ZON, Gerald ,  LAO, Kai ,  STRAUS, Neil ,  SPIER, Eugene ,  CHEN, Caifu

IPC: C12Q1/68

CPC classification number: C12Q1/6834 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/518 ,  C12Q2563/149 ,  C12Q2525/125 ,  C12Q2525/119 ,  C12Q2537/119 ,  C12Q2525/155

Abstract: Provided herein are compositions, materials, methods and kits for immobilizing a template polynucleotide in a first orientation, and immobilizing a complementary sequence of the template polynucleotide in an orientation that is flipped compared to the orientation of the template polynucleotide. Provided herein are adaptive oligonucleotides that can be used in various nucleic acid manipulations to generate immobilized complement polynucleotides that are flipped in orientation compared to the orientation of the immobilized template polynucleotides.

Abstract translation: 本文提供了用于将模板多核苷酸固定在第一取向中的组合物，材料，方法和试剂盒，并且与模板多核苷酸的取向相比，将模板多核苷酸的互补序列固定在翻转的取向中。 本文提供的适应性寡核苷酸可用于各种核酸操作以产生与固定的模板多核苷酸的取向相比翻转的固定的补体多核苷酸。

公开(公告)号：EP3626831A1

公开(公告)日：2020-03-25

申请号：EP19190463.0

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP3228715B1

公开(公告)日：2019-08-14

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

公开(公告)号：EP2812446A1

公开(公告)日：2014-12-17

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP3228715A2

公开(公告)日：2017-10-11

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合的衬底的方法包括：交换与亲脂性抗衡离子的生物分子相关的抗衡离子，以形成生物分子复合物，在非水溶剂中的生物分子复合物分散，并在非水溶剂的存在下偶联，所述生物分子复合物到基底上 。 底物或固体支持物是聚合物颗粒，要固定在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

公开(公告)号：EP2812446B1

公开(公告)日：2017-05-10

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。

公开(公告)号：EP3228715A3

公开(公告)日：2017-10-18

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.