公开(公告)号：WO2013119956A1

公开(公告)日：2013-08-15

申请号：PCT/US2013/025352

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基 的非水溶剂。 底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

公开(公告)号：WO2013119936A2

公开(公告)日：2013-08-15

申请号：PCT/US2013/025328

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： FONNUM, Geir ,  MODAHL, Grete ,  KJUS, Nini ,  MOLTEBERG, Astrid Evenroed ,  TRAN, Diem ,  AASERUD, Jo ,  GOKMEN, M. Talha ,  MENCHEN, Steven ,  FULLER, Carl ,  ANDRUZZI, Luisa ,  HINZ, Wolfgang ,  GREMYACHINSKIY, Dmitriy ,  LIGHT, David ,  MASTROIANNI, Alexander ,  ROSENBLUM, Barnett ,  KHAN, Shaheer ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C08F2/16 ,  C08F230/08

CPC classification number: C08F130/08 ,  C07F7/1852 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。

公开(公告)号：EP3626831A1

公开(公告)日：2020-03-25

申请号：EP19190463.0

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP3228715A2

公开(公告)日：2017-10-11

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合的衬底的方法包括：交换与亲脂性抗衡离子的生物分子相关的抗衡离子，以形成生物分子复合物，在非水溶剂中的生物分子复合物分散，并在非水溶剂的存在下偶联，所述生物分子复合物到基底上 。 底物或固体支持物是聚合物颗粒，要固定在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

公开(公告)号：EP2812446B1

公开(公告)日：2017-05-10

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。

公开(公告)号：EP3228715A3

公开(公告)日：2017-10-18

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP4116338A1

公开(公告)日：2023-01-11

申请号：EP22192503.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： FONNUM, Geir ,  MODAHL, Grete ,  KJUS, Nini ,  MOLTEBERG, Astrid Evenroed ,  TRAN, Diem ,  AASERUD, Jo ,  GOKMEN, M. Talha ,  MENCHEN, Steven ,  FULLER, Carl ,  ANDRUZZI, Luisa ,  HINZ, Wolfgang ,  GREMYACHINSKIY, Dmitriy ,  LIGHT, David ,  MASTROIANNI, Alexander ,  ROSENBLUM, Barnett ,  KHAN, Shaheer ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C08F2/16 ,  C08F220/54 ,  C08F8/12 ,  C08F222/38 ,  C12Q1/68 ,  C09D5/00 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F257/02 ,  C08F220/58 ,  C08F220/60 ,  C08F8/34 ,  C08F130/08 ,  C07F7/18

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：EP3228715B1

公开(公告)日：2019-08-14

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

公开(公告)号：EP2812446A1

公开(公告)日：2014-12-17

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP2812360A2

公开(公告)日：2014-12-17

申请号：EP13706818.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： FONNUM, Geir ,  MODAHL, Grete ,  KJUS, Nini ,  MOLTEBERG, Astrid Evenroed ,  TRAN, Diem ,  AASERUD, Jo ,  GOKMEN, M. Talha ,  MENCHEN, Steven ,  FULLER, Carl ,  ANDRUZZI, Luisa ,  HINZ, Wolfgang ,  GREMYACHINSKIY, Dmitriy ,  LIGHT, David ,  MASTROIANNI, Alexander ,  ROSENBLUM, Barnett ,  KHAN, Shaheer ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C08F2/16 ,  C08F220/54 ,  C08F8/12 ,  C08F222/38 ,  C12Q1/68 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F257/02 ,  C08F220/58 ,  C08F220/60 ,  C08F8/34 ,  C08F130/08 ,  C07F7/18

CPC classification number: C08F130/08 ,  C07F7/1852 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.