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    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME
    1.
    发明申请
    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME 审中-公开
    聚合聚合物颗粒及其制备方法

    公开(公告)号：WO2013119956A1

    公开(公告)日：2013-08-15

    申请号：PCT/US2013/025352

    申请日：2013-02-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

    Abstract translation: 缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基 的非水溶剂。 底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

    MATRIX ARRAYS AND METHODS FOR MAKING SAME
    2.
    发明申请
    MATRIX ARRAYS AND METHODS FOR MAKING SAME 审中-公开
    矩阵阵列及其制作方法

    公开(公告)号：WO2014151961A1

    公开(公告)日：2014-09-25

    申请号：PCT/US2014/026735

    申请日：2014-03-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIGHT, David ,  HINZ, Wolfgang ,  CICERO, Ronald ,  INMAN, Christina ,  KENNEY, Paul ,  MASTROIANNI, Alexander ,  ROZHKOV, Roman ,  WANG, Yufang ,  GRAY, Jeremy ,  GLAZER, Marc ,  GREMYACHINSKIY, Dmitriy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2523/101

    Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

    Abstract translation: 形成聚合物基质阵列的方法包括将水溶液施加到阱阵列的孔中。 该水溶液包括聚合物前体。 该方法还包括在井阵列上施加不混溶流体以将井阵列中的水溶液隔离并聚合在井阵列的孔中分离的聚合物前体以形成聚合物基质阵列。 一种装置包括传感器阵列，对应于传感器阵列的阱阵列，以及设置在阱阵列中的聚合物阵列阵列。

    PROTEIN DETECTION USING FET
    4.
    发明申请
    PROTEIN DETECTION USING FET 审中-公开
    蛋白质检测使用FET

    公开(公告)号：WO2014004587A2

    公开(公告)日：2014-01-03

    申请号：PCT/US2013/047726

    申请日：2013-06-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CONRADSON, Scott ,  DINAUER, David ,  ZHAO, Bin ,  GREMYACHINSKIY, Dmitriy ,  MYERS, Jason ,  ROSSIO, Jeffrey ,  SINGER, Victoria ,  GIORDA, Kristina

    IPC: G01N33/543

    CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

    Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

    Abstract translation: 因此，在一些实施例中，提供了用于检测一个或多个样品中的分析物或分析物的方法。 所述方法包括提供具有可寻址标记和相关配体的固体支持物，将固体基质接触样品，从而形成接触的固体支持物，将接触的固体支持物与FET阵列相关联，并检测FET阵列的电特性， 从而检测一个或多个样品中的分析物或分析物。 在其他实施例中，样本包含第二可寻址标记。

    PROTEIN DETECTION USING FET
    8.
    发明公开
    PROTEIN DETECTION USING FET 有权
    蛋白酶MIT FET

    公开(公告)号：EP2864786A2

    公开(公告)日：2015-04-29

    申请号：EP13737907.9

    申请日：2013-06-25

    Applicant: Life Technologies Corporation

    Inventor： CONRADSON, Scott ,  DINAUER, David ,  ZHAO, Bin ,  GREMYACHINSKIY, Dmitriy ,  MYERS, Jason ,  ROSSIO, Jeffrey ,  SINGER, Victoria ,  GIORDA, Kristina

    IPC: G01N33/543 ,  G01N33/569

    CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

    Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

    Abstract translation: 因此，在一些实施方案中，提供了用于检测一个或多个样品中的分析物或分析物的方法。 所述方法包括提供具有可寻址标记和相关配体的固体支持物，使固体基质与样品接触，从而形成接触的固体支持物，将接触的固体支持物与FET阵列相关联并检测FET阵列的电特性， 从而检测一个或多个样品中的分析物或分析物。 在其他实施例中，样本包含第二可寻址标记。

    HYDROPHOBIC DIACRYLAMIDE COMPOUND
    9.
    发明公开
    HYDROPHOBIC DIACRYLAMIDE COMPOUND 有权
    疏水性二丙烯酰胺化合物

    公开(公告)号：EP2812339A1

    公开(公告)日：2014-12-17

    申请号：EP13705888.9

    申请日：2013-02-08

    Applicant: Life Technologies Corporation

    Inventor： KJUS, Nini ,  FONNUM, Geir ,  BRANCHAUD, Bruce ,  YING, Lai-Qiang ,  MENCHEN, Steven ,  GREMYACHINSKIY, Dmitriy ,  KANG, Hee Chol

    IPC: C07F7/18 ,  C08F222/38

    Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

    Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，从第一溶液中分离产物，并将产物与丙烯酰氯在第二反应条件下在 第二种形成甲硅烷基保护的二丙烯酰胺化合物的溶液。

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