公开(公告)号：WO2018231818A1

公开(公告)日：2018-12-20

申请号：PCT/US2018/037090

申请日：2018-06-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DHINGRA, Dalia ,  CHIEN, Richard

IPC: C12Q1/6806

Abstract: Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.

公开(公告)号：WO2018071522A1

公开(公告)日：2018-04-19

申请号：PCT/US2017/056113

申请日：2017-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIGHT, David ,  CHEN, Shann-Ching ,  DHINGRA, Dalia ,  TOM, Warren ,  ROSENBAUM, Abraham ,  SEEGER, Collyn ,  THWAR, Prasanna

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6865

CPC classification number: C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6865 ,  C12Q2525/149 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191 ,  C12Q2525/161

Abstract: In some embodiments, the disclosure relates generally to methods, and related compositions, systems, kits and apparatuses, for conducting a stream-lined nucleic acid library preparation workflow that generally includes binding a polynucleotide (from an initial nucleic acid sample) directly to capture primers that are immobilized on a support (e.g., beads or flowcell), and amplifying the bound polynucleotide to produce a support that carries a substantially monoclonal population of the polynucleotide (or a portion thereof). The support that carries the substantially monoclonal population of the polynucleotide can be generated, using the present teachings, without performing a separate adaptor-joining step and is ready for sequencing.

Abstract translation: 在一些实施方案中，本公开内容一般涉及用于进行流线型核酸文库制备工作流程的方法和相关组合物，系统，试剂盒和设备，其通常包括结合多核苷酸（来自初始 核酸样品）直接捕获固定在载体（例如珠或流动池）上的引物，并扩增结合的多核苷酸以产生携带基本上单克隆群体的多核苷酸（或其一部分）的载体。 使用本发明的教导，可以产生携带基本上单克隆的多核苷酸群体的支持物，而无需执行单独的衔接子连接步骤并准备好测序。