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    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME
    2.
    发明申请
    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME 审中-公开
    聚合物颗粒，核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：WO2013006824A2

    公开(公告)日：2013-01-10

    申请号：PCT/US2012/045827

    申请日：2012-07-06

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIGHT, David ,  ROSENBLUM, Barnett

    Inventor： LIGHT, David ,  ROSENBLUM, Barnett

    IPC: C08F2/32 ,  C12Q1/68 ,  C08F220/56 ,  C08F6/00

    CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

    Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol% to about 20 vol%. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

    Abstract translation: 本公开涉及制备聚合物颗粒的方法，所述方法包括以下步骤：制备水性凝胶反应混合物; 在连续相中形成具有分散的凝胶反应混合物的水相胶束的乳液; 向所述连续相中加入包含至少一种聚合引发剂的引发剂油; 并在胶束中进行聚合反应。 此外，引发剂油相对于水性凝胶反应混合物的体积的体积％为约1体积％至约20体积％。 本公开还涉及制备具有相同方法步骤的核酸聚合物颗粒的方法，并且其中所述水性凝胶反应混合物包括核酸片段，例如引物。

    RAPID AMPLIFICATION OF NUCLEIC ACIDS
    4.
    发明申请
    RAPID AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
    快速扩增核酸

    公开(公告)号：WO2018071522A1

    公开(公告)日：2018-04-19

    申请号：PCT/US2017/056113

    申请日：2017-10-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIGHT, David ,  CHEN, Shann-Ching ,  DHINGRA, Dalia ,  TOM, Warren ,  ROSENBAUM, Abraham ,  SEEGER, Collyn ,  THWAR, Prasanna

    IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6865

    CPC classification number: C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6865 ,  C12Q2525/149 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191 ,  C12Q2525/161

    Abstract: In some embodiments, the disclosure relates generally to methods, and related compositions, systems, kits and apparatuses, for conducting a stream-lined nucleic acid library preparation workflow that generally includes binding a polynucleotide (from an initial nucleic acid sample) directly to capture primers that are immobilized on a support (e.g., beads or flowcell), and amplifying the bound polynucleotide to produce a support that carries a substantially monoclonal population of the polynucleotide (or a portion thereof). The support that carries the substantially monoclonal population of the polynucleotide can be generated, using the present teachings, without performing a separate adaptor-joining step and is ready for sequencing.

    Abstract translation: 在一些实施方案中，本公开内容一般涉及用于进行流线型核酸文库制备工作流程的方法和相关组合物，系统，试剂盒和设备，其通常包括结合多核苷酸（来自初始 核酸样品）直接捕获固定在载体（例如珠或流动池）上的引物，并扩增结合的多核苷酸以产生携带基本上单克隆群体的多核苷酸（或其一部分）的载体。 使用本发明的教导，可以产生携带基本上单克隆的多核苷酸群体的支持物，而无需执行单独的衔接子连接步骤并准备好测序。

    PURIFICATION SYSTEMS AND METHODS
    6.
    发明申请
    PURIFICATION SYSTEMS AND METHODS 审中-公开
    净化系统和方法

    公开(公告)号：WO2012109615A1

    公开(公告)日：2012-08-16

    申请号：PCT/US2012/024756

    申请日：2012-02-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  HINZ, Wolfgang ,  JONES, Ryan ,  LIGHT, David

    Inventor： HINZ, Wolfgang ,  JONES, Ryan ,  LIGHT, David

    IPC: C08J3/12 ,  C12N15/10 ,  B01D15/34 ,  G01N15/02

    CPC classification number: C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

    Abstract: A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

    Abstract translation: 制备水凝胶颗粒的方法包括将包含多个水凝胶颗粒的溶液施加到搅拌槽。 过滤器的滞留侧限定了搅拌槽的下表面。 过滤器具有滞留侧和渗透侧。 该方法还包括在搅拌槽内搅拌溶液的同时，以第一流速向缓冲池分配缓冲溶液，并以第二流量使用泵从过滤器的渗透侧抽出渗透物，渗透物包括 多个水凝胶颗粒的一个子集。

    MATRIX ARRAYS AND METHODS FOR MAKING SAME
    7.
    发明申请
    MATRIX ARRAYS AND METHODS FOR MAKING SAME 审中-公开
    矩阵阵列及其制作方法

    公开(公告)号：WO2014151961A1

    公开(公告)日：2014-09-25

    申请号：PCT/US2014/026735

    申请日：2014-03-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIGHT, David ,  HINZ, Wolfgang ,  CICERO, Ronald ,  INMAN, Christina ,  KENNEY, Paul ,  MASTROIANNI, Alexander ,  ROZHKOV, Roman ,  WANG, Yufang ,  GRAY, Jeremy ,  GLAZER, Marc ,  GREMYACHINSKIY, Dmitriy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2523/101

    Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

    Abstract translation: 形成聚合物基质阵列的方法包括将水溶液施加到阱阵列的孔中。 该水溶液包括聚合物前体。 该方法还包括在井阵列上施加不混溶流体以将井阵列中的水溶液隔离并聚合在井阵列的孔中分离的聚合物前体以形成聚合物基质阵列。 一种装置包括传感器阵列，对应于传感器阵列的阱阵列，以及设置在阱阵列中的聚合物阵列阵列。

    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES
    10.
    发明公开
    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES 审中-公开
    VORBEREITUNG EINER VORRICHTUNG UNTER VERWENDUNG VON KONDENSIERTENNUKLEINSÄUREPARTIKELN

    公开(公告)号：EP3044329A1

    公开(公告)日：2016-07-20

    申请号：EP14777230.5

    申请日：2014-09-12

    Applicant: Life Technologies Corporation

    Inventor： LUO, Guobin ,  SEDOVA, Marina ,  LIGHT, David ,  JONES, Ryan ,  ALANJARY, Mohammad

    IPC: C12Q1/68

    Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

    Abstract translation: 对核酸链进行测序的方法包括接收具有与聚合物基质偶联的核酸链的颗粒，将颗粒暴露于包含缩合剂的溶液中，并将颗粒施加到表面上，颗粒沉积在表面上。

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