公开(公告)号：WO2019094524A1

公开(公告)日：2019-05-16

申请号：PCT/US2018/059710

申请日：2018-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： ROSENBAUM, Abraham ,  SEEGER, Collyn ,  GRAY, Jeremy ,  YU, Hua

IPC: C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/16 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/519

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：WO2018071522A1

公开(公告)日：2018-04-19

申请号：PCT/US2017/056113

申请日：2017-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIGHT, David ,  CHEN, Shann-Ching ,  DHINGRA, Dalia ,  TOM, Warren ,  ROSENBAUM, Abraham ,  SEEGER, Collyn ,  THWAR, Prasanna

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6865

CPC classification number: C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6865 ,  C12Q2525/149 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191 ,  C12Q2525/161

Abstract: In some embodiments, the disclosure relates generally to methods, and related compositions, systems, kits and apparatuses, for conducting a stream-lined nucleic acid library preparation workflow that generally includes binding a polynucleotide (from an initial nucleic acid sample) directly to capture primers that are immobilized on a support (e.g., beads or flowcell), and amplifying the bound polynucleotide to produce a support that carries a substantially monoclonal population of the polynucleotide (or a portion thereof). The support that carries the substantially monoclonal population of the polynucleotide can be generated, using the present teachings, without performing a separate adaptor-joining step and is ready for sequencing.

Abstract translation: 在一些实施方案中，本公开内容一般涉及用于进行流线型核酸文库制备工作流程的方法和相关组合物，系统，试剂盒和设备，其通常包括结合多核苷酸（来自初始 核酸样品）直接捕获固定在载体（例如珠或流动池）上的引物，并扩增结合的多核苷酸以产生携带基本上单克隆群体的多核苷酸（或其一部分）的载体。 使用本发明的教导，可以产生携带基本上单克隆的多核苷酸群体的支持物，而无需执行单独的衔接子连接步骤并准备好测序。

公开(公告)号：WO2017196676A1

公开(公告)日：2017-11-16

申请号：PCT/US2017/031403

申请日：2017-05-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WONG, Chiu Tai Andrew ,  YANG, Xiaoling ,  SEEGER, Collyn ,  REARICK, Todd ,  MENCHEN, Steven ,  ROZHKOV, Roman ,  ROSENBLUM, Barnett ,  GRAHAM, Ronald

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2563/137 ,  C12Q2563/149 ,  C12Q2565/607

Abstract: The present disclosure provides methods relating to detecting, identifying and measuring nucleic acids that includes binding metal ions to nucleic acid polymer backbones having negative charges formed as a result of nucleotide incorporation, and detecting or measuring hydrogen ions that are generated as a result of forming metal chelate complexes by addition of chelating agents.

Abstract translation: 本公开提供了涉及检测，鉴定和测量核酸的方法，所述方法包括将金属离子结合至具有由于核苷酸掺入而形成负电荷的核酸聚合物骨架，并且检测或测量氢离子 是由于通过添加螯合剂形成金属螯合物而产生的。

公开(公告)号：EP3963103A1

公开(公告)日：2022-03-09

申请号：EP20729277.2

申请日：2020-04-30

Applicant: Life Technologies Corporation

Inventor： ROSENBAUM, Abraham ,  SEEGER, Collyn ,  GRAY, Jeremy ,  YU, Hua

IPC: C12Q1/6853

公开(公告)号：EP3707274A1

公开(公告)日：2020-09-16

申请号：EP18808182.2

申请日：2018-11-07

Applicant: Life Technologies Corporation

Inventor： ROSENBAUM, Abraham ,  SEEGER, Collyn ,  GRAY, Jeremy ,  YU, Hua

IPC: C12Q1/6806 ,  C12Q1/6874