公开(公告)号：WO2016004234A1

公开(公告)日：2016-01-07

申请号：PCT/US2015/038875

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIGHT, David ,  CICERO, Ronald L. ,  WANG, Yufang ,  MASTROIANNI, Alexander ,  THWAR, Prasanna Krishnan ,  GRAY, Jeremy ,  GLAZER, Marc ,  LINCECUM, Tommie Lloyd Jr. ,  ALANJARY, Mohammad ,  KOSCINSKI, Joseph

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

Abstract translation: 在传感器基板上加载珠粒的方法包括将包含珠粒的悬浮液施加到在传感器基底上限定的流动池中。 传感器基板包括多个孔。 珠粒至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体，从流动池中蒸发液体，例如通过抽吸空气流过流动池; 以及将水合溶液施加到所述流动池。

公开(公告)号：EP3164505A1

公开(公告)日：2017-05-10

申请号：EP15745292.1

申请日：2015-07-01

Applicant: Life Technologies Corporation

Inventor： LIGHT, David ,  CICERO, Ronald L. ,  WANG, Yufang ,  MASTROIANNI, Alexander ,  THWAR, Prasanna Krishnan ,  GRAY, Jeremy ,  GLAZER, Marc ,  LINCECUM, Tommie Lloyd Jr. ,  ALANJARY, Mohammad ,  KOSCINSKI, Joseph

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

Abstract translation: 将珠加载在传感器衬底上的方法包括将包括珠的悬浮液施加到限定在传感器衬底上的流动池。 传感器基板包括多个井。 小珠至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体，例如通过将空气吸入流动池中从流动池蒸发液体; 并将水合溶液施加到流动池。

公开(公告)号：EP3164505B1

公开(公告)日：2018-06-27

申请号：EP15745292.1

申请日：2015-07-01

Applicant: Life Technologies Corporation

Inventor： LIGHT, David ,  CICERO, Ronald L. ,  WANG, Yufang ,  MASTROIANNI, Alexander ,  THWAR, Prasanna Krishnan ,  GRAY, Jeremy ,  GLAZER, Marc ,  LINCECUM, Tommie Lloyd Jr. ,  ALANJARY, Mohammad ,  KOSCINSKI, Joseph

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.