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公开(公告)号:WO2016004244A1
公开(公告)日:2016-01-07
申请号:PCT/US2015/038894
申请日:2015-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: CICERO, Ronald L. , GLAZER, Marc , WANG, Yufang , INMAN, Christina E. , GRAY, Jeremy , KOSCINSKI, Joseph , BALL, James , WAGGONER, Phil , MASTROIANNI, Alexander
IPC: G01N27/414 , C12Q1/68 , G01N33/543
CPC classification number: G01N27/414 , G01N27/4145
Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.
Abstract translation: 传感器部件包括传感器,传感器包括与传感器协作的传感器表面和反应部位,并暴露传感器表面。 反应位点包括反应位点表面。 表面剂与反应位点表面或传感器表面结合。 表面活性剂包括在反应位点表面或传感器表面上与布朗斯台德碱或路易斯酸官能团反应的表面活性官能团,并且包括不具有供体电子对的远端官能团。
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公开(公告)号:WO2017007838A1
公开(公告)日:2017-01-12
申请号:PCT/US2016/041171
申请日:2016-07-06
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: MASTROIANNI, Alexander , BENSON, Scott C. , MENCHEN, Steven M. , SCOTT, John
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/005 , B01J2219/00545 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , C12Q1/6806 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2563/155 , C12Q2565/607 , C12Q2565/629
Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.
Abstract translation: 制备离散聚合物网络阵列的方法包括将多个核酸聚合物网络与多个着色活化聚合物网络混合以形成分散体,将该分散体施加到孔阵列上,将核酸聚合物网络选择性地沉积到孔中 的孔阵列,并且冲洗孔阵列以选择性地去除多个着色活化聚合物网络。
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公开(公告)号:WO2016004234A1
公开(公告)日:2016-01-07
申请号:PCT/US2015/038875
申请日:2015-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: LIGHT, David , CICERO, Ronald L. , WANG, Yufang , MASTROIANNI, Alexander , THWAR, Prasanna Krishnan , GRAY, Jeremy , GLAZER, Marc , LINCECUM, Tommie Lloyd Jr. , ALANJARY, Mohammad , KOSCINSKI, Joseph
IPC: C12Q1/68
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00677 , C12Q1/6806 , C12Q1/6869 , C12Q2523/32 , C12Q2527/119 , C12Q2535/122 , C12Q2563/149 , C12Q2563/155 , C12Q2565/607 , C12Q2565/629
Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.
Abstract translation: 在传感器基板上加载珠粒的方法包括将包含珠粒的悬浮液施加到在传感器基底上限定的流动池中。 传感器基板包括多个孔。 珠粒至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体,从流动池中蒸发液体,例如通过抽吸空气流过流动池; 以及将水合溶液施加到所述流动池。
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公开(公告)号:WO2012142132A1
公开(公告)日:2012-10-18
申请号:PCT/US2012/033084
申请日:2012-04-11
Applicant: LIFE TECHNOLOGIES CORPORATION , MENCHEN, Steven , MASTROIANNI, Alexander
Inventor: MENCHEN, Steven , MASTROIANNI, Alexander
CPC classification number: C12N15/1093 , C08F2/22 , C08F220/56 , C08L33/26 , Y10T428/2982
Abstract: A method of making polymer particles includes making an aqueous gel reaction mixture; forming an emulsion comprising dispersed aqueous phase micelles of gel reaction mixture in a continuous phase at a temperature less than about 10°C; and performing a polymerization reaction in the micelles. Further, the emulsion comprises at least one polymerization initiator in the micelles of gel reaction mixture. The gel reaction mixture can be maintained at a temperature less than about 10°C when it comprises the polymerization initiator.
Abstract translation: 制备聚合物颗粒的方法包括制备水凝胶反应混合物; 在小于约10℃的温度下,在连续相中形成包含分散的凝胶反应混合物的水相胶束的乳液; 并在胶束中进行聚合反应。 此外,乳液在凝胶反应混合物的胶束中包含至少一种聚合引发剂。 当凝胶反应混合物包含聚合引发剂时,可将其保持在低于约10℃的温度。
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公开(公告)号:WO2016141302A8
公开(公告)日:2016-09-09
申请号:PCT/US2016/020918
申请日:2016-03-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC: C12Q1/68 , G01N27/414
Abstract: A sensor apparatus includes a substrate, a semiconductor device (such as a chemFET or ion-sensitive FET) disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use, e.g., in nucleic acid sequencing. The semiconductor device can include a well and optionally a bead conjugated to a nucleic acid disposed in the well.
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公开(公告)号:WO2014151961A1
公开(公告)日:2014-09-25
申请号:PCT/US2014/026735
申请日:2014-03-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: LIGHT, David , HINZ, Wolfgang , CICERO, Ronald , INMAN, Christina , KENNEY, Paul , MASTROIANNI, Alexander , ROZHKOV, Roman , WANG, Yufang , GRAY, Jeremy , GLAZER, Marc , GREMYACHINSKIY, Dmitriy
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6834 , C12Q2523/101
Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.
Abstract translation: 形成聚合物基质阵列的方法包括将水溶液施加到阱阵列的孔中。 该水溶液包括聚合物前体。 该方法还包括在井阵列上施加不混溶流体以将井阵列中的水溶液隔离并聚合在井阵列的孔中分离的聚合物前体以形成聚合物基质阵列。 一种装置包括传感器阵列,对应于传感器阵列的阱阵列,以及设置在阱阵列中的聚合物阵列阵列。
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公开(公告)号:WO2013119936A2
公开(公告)日:2013-08-15
申请号:PCT/US2013/025328
申请日:2013-02-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: FONNUM, Geir , MODAHL, Grete , KJUS, Nini , MOLTEBERG, Astrid Evenroed , TRAN, Diem , AASERUD, Jo , GOKMEN, M. Talha , MENCHEN, Steven , FULLER, Carl , ANDRUZZI, Luisa , HINZ, Wolfgang , GREMYACHINSKIY, Dmitriy , LIGHT, David , MASTROIANNI, Alexander , ROSENBLUM, Barnett , KHAN, Shaheer , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC: C08F2/16 , C08F230/08
CPC classification number: C08F130/08 , C07F7/1852 , C07F7/188 , C08F2/20 , C08F2/26 , C08F6/24 , C08F8/12 , C08F8/32 , C08F8/34 , C08F220/58 , C08F222/385 , C08F230/08 , C08F2800/20 , C08J9/20 , C08J2343/04 , C12P19/34 , C12Q1/686 , C12Q1/6874 , G01N27/4145 , Y10T428/2982 , C08F257/02 , C08F2222/1013 , C08F212/36 , C08F220/60 , C12Q2563/149 , C12Q2563/159 , C12Q2565/543
Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元,从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。
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公开(公告)号:EP3489367A1
公开(公告)日:2019-05-29
申请号:EP18213839.6
申请日:2016-03-04
Applicant: Life Technologies Corporation
Inventor: WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC: C12Q1/6869 , G01N27/414
Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
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公开(公告)号:EP3766991A1
公开(公告)日:2021-01-20
申请号:EP20195062.3
申请日:2016-03-04
Applicant: Life Technologies Corporation
Inventor: WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC: C12Q1/6869 , G01N27/414
Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
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公开(公告)号:EP3249057A1
公开(公告)日:2017-11-29
申请号:EP17181624.2
申请日:2014-03-13
Applicant: Life Technologies Corporation
Inventor: LIGHT, David , HINZ, Wofgang , CICERO, Ronald , INMAN, Christina , KENNEY, Paul , MASTROIANNI, Alexander , ROZHKOV, Roman , WANG, Yufang , GRAY, Jeremy , GLAZER, Marc , GREMYACHINSKIY, Dmitriy
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6834 , C12Q1/6869 , C12Q2523/101 , C12Q2527/119 , C12Q2535/122 , C12Q2547/107 , C12Q2563/159 , C12Q2565/537 , C12Q2523/301 , C12Q2523/32
Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.
Abstract translation: 形成聚合物基质阵列的方法包括将水溶液施加到孔阵列的孔中。 水溶液包含聚合物前体。 该方法进一步包括在井阵列上施加不混溶流体以隔离井阵列的井内的水溶液,并聚合在井阵列的井中分离的聚合物前体以形成聚合物基质阵列。 一种装置包括传感器阵列,对应于传感器阵列的井阵列以及布置在井阵列中的聚合物矩阵的阵列。
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