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公开(公告)号：WO2013138724A2
公开(公告)日：2013-09-19
申请号：PCT/US2013/032107
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： PALLAS, Michael C. , NURSE, James , LIM, Gary , STRAUB, Theodore , FOSTER, Evan
IPC: B01L3/02
CPC classification number: G01N35/1002 , B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00351 , B01J2219/0038 , B01J2219/00599 , B01J2219/00619 , B01J2219/00637 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , B01L2300/165 , B01L2400/0406 , C40B60/14
Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。
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公开(公告)号：WO2013138746A1
公开(公告)日：2013-09-19
申请号：PCT/US2013/032242
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： GHENCIU, Eliodor , FOSTER, Evan , STRAUB, Theodore , PALLAS, Michael C.
CPC classification number: G01N35/1002 , B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00351 , B01J2219/0038 , B01J2219/00599 , B01J2219/00619 , B01J2219/00637 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , B01L2300/165 , B01L2400/0406 , C40B60/14
Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
Abstract translation: 提供了用于生物反应的装置。 该装置包括衬底和衬底内的多个反应位点。 衬底的表面被配置为具有第一亲水性，并且多个反应位点的每个表面被配置为具有第二亲水性，以加载具有样品体积的大量反应位点。 每个加载的反应位点的样品体积基本上限于其各自的反应位点。 样品体积被配置为在反应位点内进行生物反应。
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Patent Agency Ranking3.发明公开SYSTEMS AND METHODS FOR LOADING LIQUID SAMPLES 审中-公开SYSTEME UND VERFAHREN ZUM LADEN VONFLÜSSIGPROBEN
公开(公告)号：EP2825312A2
公开(公告)日：2015-01-21
申请号：EP13713045.6
申请日：2013-03-15
Applicant: Life Technologies Corporation
Inventor： PALLAS, Michael C. , NURSE, James , LIM,Gary , STRAUB, Theodore , FOSTER, Evan
IPC: B01L3/02
CPC classification number: G01N35/1002 , B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00351 , B01J2219/0038 , B01J2219/00599 , B01J2219/00619 , B01J2219/00637 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , B01L2300/165 , B01L2400/0406 , C40B60/14
Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
Abstract translation: 提供了用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。
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4.发明授权METHODS, SOFTWARE, AND APPARATUS FOR FOCUSING AN OPTICAL SYSTEM USING COMPUTER IMAGE ANALYSIS 有权方法，软件和设备用于聚焦光学系统利用计算机图像分析
公开(公告)号：EP1761817B1
公开(公告)日：2011-08-17
申请号：EP05764213.4
申请日：2005-06-30
Applicant: Life Technologies Corporation
Inventor： KINNEY, Patrick D. , KING, Howard G. , PALLAS, Michael C. , NALEY, Mark
CPC classification number: G02B21/244 , G02B7/38
Abstract: Methods, software, and apparatus for focusing an image in biological instrument are disclosed. Focusing elements are moved to various focus positions within a focus element travel range, and sample images are captured at the various focus positions. The sample images are resolved into subregions and an optimal focus position is determined based on the image intensity statistical dispersions within the identified subregions.
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公开(公告)号：EP2825311B1
公开(公告)日：2020-07-29
申请号：EP13716895.1
申请日：2013-03-15
Applicant: Life Technologies Corporation
Inventor： GHENCIU, Eliodor , FOSTER, Evan , STRAUB, Theodore , PALLAS, Michael C.
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6.发明公开IMPROVED THERMAL UNIFORMITY FOR THERMAL CYCLER INSTRUMENTATION USING DYNAMIC CONTROL 审中-公开利用动态控制改善热循环器仪表的热一致性
公开(公告)号：EP2556173A2
公开(公告)日：2013-02-13
申请号：EP11766806.1
申请日：2011-04-08
Applicant: Life Technologies Corporation
Inventor： CONNER, Thomas A. , LIM, Chee Kiong , PALLAS, Michael C. , CHING, Chee Wee
CPC classification number: B01L7/52 , B01L2200/147 , B01L2300/1822 , B01L2300/1894
Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.
Abstract translation: 提供了用于进行聚合酶链式反应（PCR）以改善热不均匀性的方法。 该方法包括：通过第一传感器测量样本块的第一样本块扇区的第一温度，并且通过第二传感器测量样本块的与第一样本块相邻的第二样本块扇区的第二温度 样本块部门。 该方法进一步包括通过热电控制器计算第一温度和第二温度之间的温度差并且通过热电控制器基于温度差通过使用一个或多个温度计来调整第一样本块扇区的第一温度 更多的热电冷却器。 一个或多个热电冷却器被配置为通过调整来自热电控制器的功率输出来加热或冷却第一样本块扇区。
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公开(公告)号：EP2825311A1
公开(公告)日：2015-01-21
申请号：EP13716895.1
申请日：2013-03-15
Applicant: Life Technologies Corporation
Inventor： GHENCIU, Eliodor , FOSTER, Evan , STRAUB, Theodore , PALLAS, Michael C.
Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/−15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
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