公开(公告)号：WO2017087842A1

公开(公告)日：2017-05-26

申请号：PCT/US2016/062844

申请日：2016-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： POTTER, Robert Jason ,  RAVINDER, Namritha ,  CARTE, Jason

IPC: C12N15/10 ,  C12N9/96

CPC classification number: C12N15/1003 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22

Abstract: The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration ( e.g ., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification ( e.g ., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.

Abstract translation: 本公开总体上涉及用于细胞遗传修饰的组合物和方法。 具体而言，本公开涉及用于基因组改变（例如，位点特异性核酸酶）的稳定化试剂以及用于非遗传密码改变修饰（例如，）的稳定化试剂。 ，DNA甲基化）。 稳定化方法包括在合适的温度下储存试剂，转化为干燥形式和化学修饰。

公开(公告)号：WO2017066707A1

公开(公告)日：2017-04-20

申请号：PCT/US2016/057224

申请日：2016-10-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： XU, Xin ,  LIANG, Xiquan ,  DE MOLLERAT DU JEU, Xavier ,  POTTER, Robert Jason

IPC: A61K48/00 ,  C12N15/88

CPC classification number: C12N15/90 ,  A61K48/0033 ,  A61K48/005 ,  C12N9/22 ,  C12N15/88 ,  C12N2830/30 ,  C12Y301/00

Abstract: Provided herein are compositions and methods useful, inter alia , for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

Abstract translation: 本文提供了用于将核糖核蛋白复合物（例如Cas9 /引导RNA复合物）递送入细胞的组合物和方法，尤其是用于其他方面的组合物和方法。 本文提供的组合物和方法对于将核糖核蛋白复合物递送到多能细胞和淋巴细胞中是特别有用的。

公开(公告)号：EP3362102A1

公开(公告)日：2018-08-22

申请号：EP16790794.8

申请日：2016-10-14

Applicant: Life Technologies Corporation

Inventor： YU, Xin ,  LIANG, Xiquan ,  DE MOLLERAT DU JEU, Xavier ,  POTTER, Robert Jason

IPC: A61K48/00 ,  C12N15/88

CPC classification number: C12N15/90 ,  A61K48/0033 ,  A61K48/005 ,  C12N9/22 ,  C12N15/88 ,  C12N2830/30 ,  C12Y301/00

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

公开(公告)号：EP3377626A1

公开(公告)日：2018-09-26

申请号：EP16819717.6

申请日：2016-11-18

Applicant: Life Technologies Corporation

Inventor： POTTER, Robert Jason ,  RAVINDER, Namritha ,  CARTE, Jason

IPC: C12N15/10 ,  C12N9/96

CPC classification number: C12N15/1003 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22

Abstract: The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration (e.g., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification (e.g., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.