公开(公告)号：CA2689626C

公开(公告)日：2016-10-25

申请号：CA2689626

申请日：2008-06-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  MURPHY DEVON ,  HOLDEN DAVID ,  HESTER KEVIN ,  MARKS PATRICK ,  TOMANEY AUSTIN B

IPC: C12Q1/68 ,  C12M1/34 ,  C12P19/34 ,  G06F19/20

Abstract: Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by- incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof.

公开(公告)号：AU2011326026A1

公开(公告)日：2013-05-02

申请号：AU2011326026

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2009297145A1

公开(公告)日：2010-04-01

申请号：AU2009297145

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：AU2008261935B2

公开(公告)日：2013-05-02

申请号：AU2008261935

申请日：2008-06-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  HOLDEN DAVID ,  MARKS PATRICK ,  HESTER KEVIN ,  MURPHY DEVON ,  TOMANEY AUSTIN B

IPC: C12M1/34 ,  C12P19/34 ,  C12Q1/68

Abstract: Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by- incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof.