公开(公告)号：CA2746632C

公开(公告)日：2020-06-30

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2009297145A1

公开(公告)日：2010-04-01

申请号：AU2009297145

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：AU2011326026A1

公开(公告)日：2013-05-02

申请号：AU2011326026

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2009325069B2

公开(公告)日：2015-03-19

申请号：AU2009325069

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.