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    METHODS FOR PEPTIDE MAPPING OF ADENO-ASSOCIATED VIRUS (AAV) PROTEINS
    1.
    发明申请
    METHODS FOR PEPTIDE MAPPING OF ADENO-ASSOCIATED VIRUS (AAV) PROTEINS 审中-公开

    公开(公告)号：WO2022175728A1

    公开(公告)日：2022-08-25

    申请号：PCT/IB2021/053914

    申请日：2021-05-07

    Applicant: WATERS TECHNOLOGIES CORPORATION

    Inventor： ZHANG, Ximo ,  KOZA, Stephan M. ,  YU, Ying Qing ,  CHEN, Weibin

    IPC: G01N33/68

    Abstract: The present disclosure relates to a method of characterizing proteins in a sample. The method includes: removing non-ionic surfactant from the sample via denaturing size-exclusion chromatography to form a denatured sample; eluting the denatured sample via liquid chromatography to collect fractions of the sample, wherein the fractions of the sample include a protein fraction; lyophilizing the protein fraction to increase protein concentration; reconstituting the lyophilized protein fraction with a buffer comprising a surfactant to denature the protein; digesting the denatured protein fraction with an enzyme; and analyzing the digested protein fraction.

    LOW BINDING SURFACES FOR PEPTIDE MAPPING
    2.
    发明申请
    LOW BINDING SURFACES FOR PEPTIDE MAPPING 审中-公开

    公开(公告)号：WO2021144765A1

    公开(公告)日：2021-07-22

    申请号：PCT/IB2021/050318

    申请日：2021-01-16

    Applicant: WATERS TECHNOLOGIES CORPORATION

    Inventor： BIRDSALL, Robert ,  KELLETT, Jacob ,  RANBADUGE, Nilini ,  YU, Ying Qing ,  NGUYEN, Jennifer M. ,  LAUBER, Matthew A.

    IPC: G01N30/56 ,  G01N2030/567 ,  G01N2030/8831

    Abstract: The present disclosure discusses a method of separating a sample (e.g., peptide compound) including coating a flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample – and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating (e.g., organosilica coating) of the flow path. The applied coating can reduce peak tailing and increase analyte recovery for the sample of the chromatographic system.

    METHODS, COMPOSITIONS AND KITS USEFUL FOR ION EXCHANGE CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS
    5.
    发明申请
    METHODS, COMPOSITIONS AND KITS USEFUL FOR ION EXCHANGE CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS 审中-公开

    公开(公告)号：WO2020185546A1

    公开(公告)日：2020-09-17

    申请号：PCT/US2020/021352

    申请日：2020-03-06

    Applicant: WATERS TECHNOLOGIES CORPORATION

    Inventor： WANG, Qi ,  LAUBER, Matthew A. ,  IPPOLITI, Samantha ,  YU, Ying Qing

    IPC: G01N30/96 ,  G01N30/34

    Abstract: The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution kits comprising (a) a first aqueous buffer solution having a first pH and comprising a first organic acid salt in a first concentration and (b) a second aqueous buffer solution having a second pH and comprising the first organic acid salt in a second concentration, wherein the first organic acid salt comprises a first organic acid ammonium salt, wherein the second pH is greater than the first pH, and wherein the second concentration is greater than the first concentration. In various aspects, the present disclosure pertains to methods of using such aqueous buffer solutions in chromatographic separations.

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