公开(公告)号：US20150152475A1

公开(公告)日：2015-06-04

申请号：US14480512

申请日：2014-09-08

Applicant: Bio-Rad Laboratories

Inventor： Igor Kordunsky ,  Jeffrey A. Goldman ,  Michael J. Finney

IPC: C12Q1/68 ,  B01L7/00 ,  G01N21/64

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1822 ,  G01N21/276 ,  G01N21/6452 ,  G01N2201/0446

Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.

Abstract translation: 提供了一种用于分析位于热循环仪中的多个孔中的样品和使用方法的荧光检测装置。 在一个实施例中，该装置包括可附接到热循环仪的支撑结构和可移动地安装在支撑结构上的检测模块。 检测模块包括一个或多个通道，每个通道具有设置在检测模块内的激发光发生器和发射光检测器。 当支撑结构附接到热循环仪并且检测模块安装在支撑结构上时，检测模块可移动以便定位成与多个井中的不同井的光通信。 检测模块可从支撑结构移除，以方便更换。

公开(公告)号：US20120099098A1

公开(公告)日：2012-04-26

申请号：US13265969

申请日：2010-04-23

Applicant: Simon Webster

Inventor： Simon Webster

IPC: G01N21/01 ,  G01N21/64 ,  G01J3/44 ,  G01J3/28 ,  G01N21/59 ,  G01N21/47

CPC classification number: G01N21/03 ,  G01N21/0332 ,  G01N2021/0346 ,  G01N2201/0446

Abstract: A unit is provided comprising an array (2) of sample containers (1), said containers, being connected together and arranged in a planar configuration, each container having multiple optically transparent windows arranged such that the sample contained therein can be interrogated using simultaneous multiple optical analytical techniques, the array of containers being configured so as to allow optical access to the windows of each container in the array. Also provided is an apparatus comprising such a unit, a system comprising a combination of such an apparatus and unit and a method of analysing multiple samples by introducing each individual sample into an individual container of such an apparatus, illuminating the samples and detecting and analysing light emerging therefrom.

Abstract translation: 提供了包括样品容器（1）的阵列（2），所述容器连接在一起并布置成平面构造的单元，每个容器具有多个光学透明窗口，其布置使得其中容纳的样品可以使用同时多个 光学分析技术，容器阵列被配置为允许光学访问阵列中每个容器的窗口。 还提供了一种包括这种单元的装置，包括这种装置和单元的组合的系统以及通过将每个单独的样品引入这种装置的单独容器中来分析多个样品的方法，照亮样品并检测和分析光 从中出现。

公开(公告)号：US20120064564A1

公开(公告)日：2012-03-15

申请号：US13227761

申请日：2011-09-08

Applicant: JOSEF GRASSL ,  Gerald Probst ,  Markus Schürf

Inventor： JOSEF GRASSL ,  Gerald Probst ,  Markus Schürf

IPC: C12Q1/02 ,  C12M1/34 ,  G01N21/17 ,  G01N21/00 ,  G01N21/64

CPC classification number: G01N35/028 ,  B01L9/52 ,  C12M1/04 ,  C12M41/14 ,  C12M41/16 ,  C12Q1/02 ,  G01J3/12 ,  G01N21/253 ,  G01N21/6452 ,  G01N33/5005 ,  G01N2035/00356 ,  G01N2201/023 ,  G01N2201/0231 ,  G01N2201/0238 ,  G01N2201/0446 ,  G01N2201/06113

Abstract: A microplate reader and method has at least one measuring device and a holding device for accommodating at least one microplate and for positioning samples-containing wells of microplates in relation to the measuring device. The at least one measuring device is used for detecting light emitted by samples in wells of a microplate and/or which is influenced by samples transilluminated by light in the wells. The microplate reader has a control unit for controlling the composition of a gas atmosphere surrounding the wells containing the samples. A respective use is characterized particularly in that living cells are measured in a controlled gas atmosphere, wherein the living cells are chosen from microaerophilic, optionally anaerobic and obligatorily anaerobic microorganisms as well as fungi and eukaryotic cells.

Abstract translation: 微板读数器和方法具有至少一个测量装置和保持装置，用于容纳至少一个微孔板并且用于相对于测量装置定位含有微孔板的样品孔。 所述至少一个测量装置用于检测由微孔板的孔中的样品发射的光和/或受孔中由光透射的样品的影响。 微板读数器具有控制单元，用于控制包含样品的孔周围的气体气氛的组成。 各自的用途的特征在于，在受控气体气氛中测量活细胞，其中活细胞选自微需氧，任选的厌氧和强制性厌氧微生物以及真菌和真核细胞。

公开(公告)号：US20070114444A1

公开(公告)日：2007-05-24

申请号：US11451903

申请日：2006-06-12

Applicant: Taylor Reid ,  Roger Taylor ,  Kenneth Zajac

Inventor： Taylor Reid ,  Roger Taylor ,  Kenneth Zajac

IPC: G01T1/10 ,  G01B9/10 ,  G01J1/58 ,  F21V9/16

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光发射到照明管中的光源; 多个收集光学器件，位于照明管中用于收集荧光的孔周围; 以及用于确定荧光量的检测器。 检测荧光的方法包括将来自光源的激发光发射到照明管中; 将激发光引导到激发滤光器; 用激发光照射样品以产生发射光; 以及使用位于照明管周围的多个收集光学器件来检测发射光的光学特性。

公开(公告)号：US20070059754A1

公开(公告)日：2007-03-15

申请号：US11555642

申请日：2006-11-01

Applicant: Igor Kordunsky ,  Jeffrey Goldman ,  Michael Finney

Inventor： Igor Kordunsky ,  Jeffrey Goldman ,  Michael Finney

IPC: C12Q1/68 ,  C12P19/34 ,  C12M3/00

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1822 ,  G01N21/276 ,  G01N21/6452 ,  G01N2201/0446

Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.

Abstract translation: 提供了用于分析位于热循环仪中的多个孔中的样品的荧光检测装置和使用方法。 在一个实施例中，该装置包括可附接到热循环仪的支撑结构和可移动地安装在支撑结构上的检测模块。 检测模块包括一个或多个通道，每个通道具有设置在检测模块内的激发光发生器和发射光检测器。 当支撑结构附接到热循环仪并且检测模块安装在支撑结构上时，检测模块可移动以便定位成与多个井中的不同井的光通信。 检测模块可从支撑结构移除，以方便更换。

公开(公告)号：US20060281183A1

公开(公告)日：2006-12-14

申请号：US11408612

申请日：2006-04-20

Applicant: Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: G01N35/00

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: Systems for managing workflows to perform chemical or biological reactions using microfluidic devices.

Abstract translation: 用于管理使用微流体装置进行化学或生物反应的工作流的系统。

公开(公告)号：US20050059158A1

公开(公告)日：2005-03-17

申请号：US10700864

申请日：2003-11-03

Applicant: Ventzeslav Iordanov ,  Pasqualina Sarro ,  Reinoud Wolffenbuttel ,  Michael Vellekoop

Inventor： Ventzeslav Iordanov ,  Pasqualina Sarro ,  Reinoud Wolffenbuttel ,  Michael Vellekoop

IPC: C12Q1/26 ,  C12Q1/32 ,  C12Q1/48 ,  G01N21/03 ,  G01N21/25 ,  G01N21/64 ,  G01N21/78 ,  G01N33/551 ,  G01N33/552 ,  G01N33/553 ,  G02B5/20 ,  G01N21/00

CPC classification number: G01N21/6452 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/485 ,  G01N21/0303 ,  G01N21/6428 ,  G01N33/551 ,  G01N33/552 ,  G01N33/553 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2201/0446 ,  G02B5/207 ,  G02B5/208

Abstract: The invention relates to a method of performing a fluorescence assay. According to the invention a filter is integrated with at least one component chosen from i) a body comprising a well, wherein the well in which the assay is being performed is exposed to excitation light in such a manner that the filter is positioned between the light-sensitive element and the excitation light source, and ii) a light-sensitive element, wherein the filter is applied at least at the light-sensitive side of the surface of the light-sensitive element, and any light that may be emitted is detected by means of the light-sensitive element. The invention also relates to an apparatus suitable for carrying out the method, as well as a method for its manufacture.

Abstract translation: 本发明涉及进行荧光测定的方法。 根据本发明，过滤器与至少一种选自i）包括井的主体的组件整合在一起，其中将进行测定的井暴露于激发光，使得过滤器位于光 感光元件和激发光源，以及ii）感光元件，其中至少在感光元件的表面的感光侧施加滤光片，并且检测到可能发射的任何光 借助于光敏元件。 本发明还涉及一种适于实施该方法的装置及其制造方法。

公开(公告)号：US20050030541A1

公开(公告)日：2005-02-10

申请号：US10878895

申请日：2004-06-28

Applicant: Andreas Erlbacher ,  Lutz Niggl ,  Alois Krutzenbichler

Inventor： Andreas Erlbacher ,  Lutz Niggl ,  Alois Krutzenbichler

IPC: B01L3/00 ,  G01N21/03 ,  G01N21/25 ,  G01N21/53 ,  G01N21/59 ,  G01N21/64 ,  G01N21/65 ,  G02F1/03 ,  G01N21/00 ,  G02F1/07

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/168 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/59 ,  G01N2021/6463 ,  G01N2021/6491 ,  G01N2021/651 ,  G01N2201/0446 ,  G01N2201/1042 ,  Y10S436/809

Abstract: The present invention relates to a device (1) and a method for determining parameters of fluid-containing samples (2) in a system (3) for individually irradiating the samples (2) with light (4) of a light source (5) in an essentially vertical irradiation direction (6). In this case, this system (3) includes a detector (7) for measuring the light (8) coming from a single sample, and this detector (7) has a detection direction (9′), which lies on an optical axis (9) that is essentially parallel to the optical axis (6) of the light source (5). This device (1) includes at least one reflective surface (10), using which the light (4) coming essentially vertically out of the light source (5) may be at least partially deflected in an essentially horizontal irradiation direction (11). The device according to the present invention and the method according to the present invention are distinguished in that the detection direction (9′) of the detector (7)—for measuring the individual light (8) coming from a single sample (2)—is positioned at an angle to the optical axis of the light (4) irradiating the sample (2) in such a way that only the light (8) coming from the individual sample (2), but not this light (4), reaches the detector (7).

Abstract translation: 本发明涉及一种用于在系统（3）中确定用于用光源（5）的光（4）单独照射样品（2）的系统（3）中的含液样品（2）的参数的装置（1）和方法， 在基本垂直的照射方向（6）上。 在这种情况下，该系统（3）包括用于测量来自单个样品的光（8）的检测器（7），并且该检测器（7）具有位于光轴（9'）上的检测方向（9' 9），其基本上平行于光源（5）的光轴（6）。 该装置（1）包括至少一个反射表面（10），基本上垂直离开光源（5）的光线可以在基本上水平的照射方向（11）上至少部分地偏转。 根据本发明的装置和根据本发明的方法的特征在于，用于测量来自单个样品（2）的单个光（8）的检测器（7）的检测方向（9'） - 被定位成与照射样品（2）的光（4）的光轴成一定角度，使得仅来自各个样品（2）的光（8）而不是该光（4）到达 检测器（7）。

公开(公告)号：US20030081207A1

公开(公告)日：2003-05-01

申请号：US10266062

申请日：2002-10-07

Inventor： Mark Wechsler ,  Howard H. Barney ,  Roger A. Kaye ,  David G. Ogle ,  Michael M. Lacy ,  Calvin Y. Chow ,  Kimberly L. Crawford ,  Dean G. Hafeman

IPC: G01J003/30

CPC classification number: G01N21/33 ,  G01N21/253 ,  G01N21/31 ,  G01N35/028 ,  G01N2201/0231 ,  G01N2201/0446 ,  G01N2201/1222 ,  G01N2201/127

Abstract: The present invention relates to a photometric device for measuring optical parameters. The invention functions in the ultraviolet light range through use of a monochromator and splits the test light in multiple channels by a rotor assembly, including a mirror.

Abstract translation: 本发明涉及一种用于测量光学参数的测光装置。 本发明通过使用单色仪在紫外光范围内起作用，并通过包括反射镜的转子组件在多个通道中分离测试光。

公开(公告)号：US5557398A

公开(公告)日：1996-09-17

申请号：US228436

申请日：1994-04-15

Applicant: Mark Wechsler ,  Howard H. Barney ,  Roger A. Kaye ,  David G. Ogle ,  Michael M. Lacy ,  Calvin Y. Chow ,  Kimberly L. Crawford ,  Dean G. Hafeman

Inventor： Mark Wechsler ,  Howard H. Barney ,  Roger A. Kaye ,  David G. Ogle ,  Michael M. Lacy ,  Calvin Y. Chow ,  Kimberly L. Crawford ,  Dean G. Hafeman

IPC: G01N21/33 ,  G01N21/25 ,  G01N21/31 ,  G01N21/59 ,  G01N35/02 ,  G01J3/30

CPC classification number: G01N21/33 ,  G01N21/253 ,  G01N21/31 ,  G01N2201/0231 ,  G01N2201/0446 ,  G01N2201/1222 ,  G01N2201/127 ,  G01N35/028

Abstract: The present invention relates to a photometric device for measuring optical parameters. The invention functions in the ultraviolet light range through use of a monochromator and splits the test light in multiple channels by a rotor assembly, including a mirror.