公开(公告)号：US20010033796A1

公开(公告)日：2001-10-25

申请号：US09796871

申请日：2001-02-28

Inventor： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC: F04B017/00 ,  F04B001/00

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：US5976826A

公开(公告)日：1999-11-02

申请号：US951886

申请日：1997-10-16

Applicant: Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

Inventor： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC: B01J19/00 ,  B81C1/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C40B60/14 ,  C12Q1/02 ,  C12N11/02 ,  C12N11/08 ,  C12Q1/04

CPC classification number: C12N5/067 ,  B01J19/0046 ,  B81C1/00206 ,  B82Y30/00 ,  B82Y5/00 ,  C12N5/0068 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C2201/034 ,  C12N2535/10 ,  C40B60/14

Abstract: A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.

Abstract translation: 提供了用于将细胞粘附在特定和预定位置的装置。 该装置包括限定表面的板和多个细胞亲和岛，所述细胞亲和岛附着细胞，细胞被细胞悬浮区域分离，细胞不粘附并进一步与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。 此外，细胞疏气区域足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。 该装置用于在表面或培养基中培养细胞并用于进行细胞计数的方法。 此外，该装置用于在表面固定细胞并用于控制细胞的形状。

公开(公告)号：US5776748A

公开(公告)日：1998-07-07

申请号：US659537

申请日：1996-06-06

Applicant: Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

Inventor： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC: B01J19/00 ,  B81C1/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C40B60/14 ,  B32B31/00 ,  C12N11/08 ,  C12N11/14

CPC classification number: C12N5/067 ,  B01J19/0046 ,  B81C1/00206 ,  B82Y30/00 ,  B82Y5/00 ,  C12N5/0068 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C2201/034 ,  C12N2535/10 ,  C40B60/14

Abstract: The disclosed invention is a device for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.

Abstract translation: 所公开的发明是用于在特定和预定位置粘附电池的装置。 该装置包括限定表面的板和多个细胞亲和岛，所述细胞亲和岛附着细胞，所述细胞由细胞不粘附的细胞渗透区域分离，并且还与细胞色素岛连续。 岛或区域或两者可以由自组装单层（SAM）形成。 此外，细胞疏气区域足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。 该装置用于在表面或培养基中培养细胞并用于进行细胞计数的方法。 此外，该装置用于在表面固定细胞并用于控制细胞的形状。

公开(公告)号：US11690989B2

公开(公告)日：2023-07-04

申请号：US16201300

申请日：2018-11-27

Applicant: Nissha Co., Ltd.

Inventor： Hiroyuki Nagai ,  Kensaku Akita ,  Masateru Chiyama

IPC: B29C45/14 ,  A61M37/00 ,  B81B1/00 ,  B29C39/10 ,  B81C1/00 ,  B29K101/12 ,  B29L31/00

CPC classification number: A61M37/0015 ,  A61M37/00 ,  B29C39/10 ,  B29C45/14 ,  B81B1/00 ,  B81B1/008 ,  B81C1/00 ,  B81C1/00111 ,  A61M2037/0023 ,  A61M2037/0046 ,  A61M2037/0053 ,  B29K2101/12 ,  B29L2031/756 ,  B29L2031/7544 ,  B81B2203/0361 ,  B81C2201/034

Abstract: Provided is an inexpensive microneedle array with little dimensional error that can control, with high precision, the amount of a predetermined component to be introduced to the inner part of the skin, and a production method for this microneedle array. A foundation that is insoluble or sparingly soluble in inner part of the skin is overlaid on a mold. A plurality of frustum-shaped protrusions, which are insoluble or sparingly soluble in the raw material liquid, provided on a first main surface of the foundation are fit into a plurality of cone-shaped recesses. The raw material liquid in the plurality of cone-shaped recesses dries and, as a result, a plurality of microneedles, which are dissolvable in the inner part of the skin, are fixed to tip surfaces of the plurality of frustum-shaped protrusions.

公开(公告)号：US09796586B2

公开(公告)日：2017-10-24

申请号：US14985859

申请日：2015-12-31

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Khalil Najafi ,  Tal Nagourney ,  Jae Yoong Cho

IPC: C03B23/035 ,  B22D25/02 ,  B81C99/00 ,  B22D23/06 ,  C03B23/02 ,  C03B23/03

CPC classification number: B81C99/0085 ,  B22D23/06 ,  B22D25/02 ,  B81C2201/034 ,  C03B23/02 ,  C03B23/0302 ,  C03B23/0352 ,  C03B23/0355 ,  C03B23/0357

Abstract: A method for fabricating three-dimensional microstructures is presented. The method includes: disposing a substantially planar reflow material between two molds; heating the reflow material while the reflow material is disposed between the two molds; and reflowing the reflow material towards the bottom surface of one of the molds by creating a pressure gradient across the reflow material. At least one of molds includes geometrics features that help to shape the reflow material and thereby form a complex three-dimensional microstructure.

公开(公告)号：US20150325329A1

公开(公告)日：2015-11-12

申请号：US14804567

申请日：2015-07-21

Applicant: Liquidia Technologies, Inc.

Inventor： Joseph M. Desimone ,  Robert Lyon Henn ,  Jacob J. Sprague

IPC: H01B1/08 ,  H01B13/00 ,  H01F1/01 ,  H01B1/02

CPC classification number: H01B1/08 ,  B81C99/0095 ,  B81C2201/034 ,  B81C2201/038 ,  H01B1/02 ,  H01B13/00 ,  H01F1/01 ,  Y10T428/2982

Abstract: A plurality of nanoparticles, a structure assembled therefrom, a method of forming the structure, including a plurality of particles where each particle of the plurality of particles is configured with a substantially predetermined shape and a largest dimension less than about 100 micrometers, and where each particle of the plurality of particles includes an opening through the particle.

Abstract translation: 多个纳米颗粒，由其组装的结构，形成该结构的方法，包括多个颗粒，其中多个颗粒的每个颗粒被构造成具有基本上预定的形状和最小尺寸小于约100微米，并且其中每个 多个颗粒的颗粒包括穿过颗粒的开口。

公开(公告)号：US08851442B2

公开(公告)日：2014-10-07

申请号：US12017944

申请日：2008-01-22

Applicant: Robert J. Carlson

Inventor： Robert J. Carlson

IPC: B29C33/56 ,  B81C1/00 ,  B81C99/00

CPC classification number: B29C33/3842 ,  B29C41/00 ,  B29C59/14 ,  B29C59/16 ,  B29K2909/00 ,  B29K2995/0037 ,  B81B7/00 ,  B81B2201/05 ,  B81B2201/058 ,  B81B2203/0127 ,  B81C99/009 ,  B81C2201/0132 ,  B81C2201/0143 ,  B81C2201/034 ,  C23F1/02 ,  H01L21/30604 ,  Y10T428/24496

Abstract: The invention is directed to a patterned aerogel-based layer that serves as a mold for at least part of a microelectromechanical feature. The density of an aerogel is less than that of typical materials used in MEMS fabrication, such as poly-silicon, silicon oxide, single-crystal silicon, metals, metal alloys, and the like. Therefore, one may form structural features in an aerogel-based layer at rates significantly higher than the rates at which structural features can be formed in denser materials. The invention further includes a method of patterning an aerogel-based layer to produce such an aerogel-based mold. The invention further includes a method of fabricating a microelectromechanical feature using an aerogel-based mold. This method includes depositing a dense material layer directly onto the outline of at least part of a microelectromechanical feature that has been formed in the aerogel-based layer.

Abstract translation: 本发明涉及用作至少部分微机电特征的模具的图案化气凝胶基层。 气凝胶的密度小于MEMS制造中使用的典型材料的密度，例如多晶硅，氧化硅，单晶硅，金属，金属合金等。 因此，可以以明显高于在较致密的材料中形成结构特征的速率的速率在气凝胶层中形成结构特征。 本发明还包括一种图案化气凝胶层以产生这种基于气凝胶的模具的方法。 本发明还包括使用基于气凝胶的模具制造微机电特征的方法。 该方法包括将致密材料层直接沉积在已经形成在气凝胶层中的微机电特征的至少一部分的轮廓上。

公开(公告)号：US08808588B1

公开(公告)日：2014-08-19

申请号：US12012653

申请日：2008-02-04

Applicant: Blake Simmons ,  Linda Domeier ,  Noble Woo ,  Timothy Shepodd ,  Ronald F. Renzi

Inventor： Blake Simmons ,  Linda Domeier ,  Noble Woo ,  Timothy Shepodd ,  Ronald F. Renzi

IPC: G01N15/06 ,  G01N33/00

CPC classification number: F16K99/0001 ,  B81C3/002 ,  B81C2201/034 ,  F15C5/00 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  Y10T436/11 ,  Y10T436/2575

Abstract: Injection molding is used to form microfluidic devices with integrated functional components. One or more functional components are placed in a mold cavity, which is then closed. Molten thermoplastic resin is injected into the mold and then cooled, thereby forming a solid substrate including the functional component(s). The solid substrate including the functional component(s) is then bonded to a second substrate, which may include microchannels or other features.

Abstract translation: 注射成型用于形成具有集成功能组件的微流体装置。 将一个或多个功能部件放置在模腔中，然后将其封闭。 将熔融的热塑性树脂注入模具中，然后冷却，从而形成包括功能组分的固体基材。 然后将包括功能组分的固体基质结合到第二基底，其可以包括微通道或其它特征。

公开(公告)号：US08741380B2

公开(公告)日：2014-06-03

申请号：US12859802

申请日：2010-08-20

Applicant: Hiroshi Yoshida ,  Haruo Akahoshi ,  Akihiro Miyauchi ,  Masahiko Ogino

Inventor： Hiroshi Yoshida ,  Haruo Akahoshi ,  Akihiro Miyauchi ,  Masahiko Ogino

IPC: A61M5/00 ,  A61M5/32

CPC classification number: B29C33/3842 ,  B29C33/424 ,  B81B2203/0361 ,  B81C1/00111 ,  B81C1/00206 ,  B81C99/0085 ,  B81C99/009 ,  B81C2201/034 ,  B82Y10/00 ,  B82Y40/00 ,  C23C18/1608 ,  C23C18/1651 ,  C23C18/1653 ,  C23C18/1657 ,  C23C18/1689 ,  C23C18/208 ,  C23C18/42 ,  G03F7/0002 ,  H01J1/304 ,  H01J9/025 ,  Y10T428/12389 ,  Y10T428/12993

Abstract: A fine metal structure having its surface furnished with microprojections of high strength, high precision and large aspect ratio; and a process for producing the fine metal structure free of defects. There is provided a fine metal structure having its surface furnished with microprojections, characterized in that the microprojections have a minimum thickness or minimum diameter ranging from 10 nanometers to 10 micrometers and that the ratio between minimum thickness or minimum diameter (D) of microprojections and height of microprojections (H), H/D, is greater than 1. There is further provided a process for producing a fine metal structure, characterized by comprising providing a substrate having a fine rugged pattern on its surface, applying a molecular electroless plating catalyst to the surface, thereafter carrying out electroless plating to thereby form a metal layer having the rugged pattern filled, and detaching the metal layer from the substrate to thereby obtain a fine metal structure furnished with a surface having undergone reversal transfer of the above rugged pattern.

Abstract translation: 精细的金属结构，其表面具有高强度，高精度和大纵横比的微喷射体; 以及没有缺陷的金属微细结构的制造方法。 提供了具有微喷射体的表面的细金属结构，其特征在于，微喷射体具有10纳米至10微米的最小厚度或最小直径，并且微喷射体的最小厚度或最小直径（D）与高度 的微喷射体（H）H / D大于1.还提供了一种制造精细金属结构的方法，其特征在于，在其表面上提供具有细凹凸图案的基板，将分子无电镀催化剂 然后进行无电解电镀，从而形成具有凹凸图案的金属层，并将金属层与基板分离，从而获得配备有经过上述凹凸图案的反转传送的表面的精细金属结构。

公开(公告)号：US08695640B2

公开(公告)日：2014-04-15

申请号：US13360261

申请日：2012-01-27

Applicant: Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

Inventor： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC: F15C1/06

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。