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公开(公告)号：US09400365B2
公开(公告)日：2016-07-26
申请号：US14728685
申请日：2015-06-02
Applicant: CELLOMICS, INC.
Inventor： Dirk John Vandenberg, III , Keith R. Heffley
CPC classification number: G02B7/003 , G02B21/00 , G02B26/008 , Y10T29/49817 , Y10T29/49826
Abstract: A filter wheel assembly includes a filter wheel and an optical assembly. The filter wheel includes a plurality of viewing openings. The optical assembly includes an optical element secured within an aperture of a housing. The optical assembly is securable to the filter wheel at a viewing opening by a magnetic force. An optical apparatus includes an optical assembly receiver and first and second optical assemblies. The first optical assembly is securable to the optical assembly receiver and the second optical assembly is securable to the first optical assembly by a magnetic force to align the optical assembly receiver and the optical assemblies.
Abstract translation: 滤光轮组件包括滤光轮和光学组件。 滤光轮包括多个观察孔。 光学组件包括固定在壳体的孔内的光学元件。 光学组件通过磁力固定在观察开口处的滤光轮上。 光学装置包括光学组件接收器和第一和第二光学组件。 第一光学组件可固定到光学组件接收器，并且第二光学组件通过磁力固定到第一光学组件，以对准光学组件接收器和光学组件。
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公开(公告)号：US09201062B2
公开(公告)日：2015-12-01
申请号：US14334453
申请日：2014-07-17
Applicant: CELLOMICS, INC.
Inventor： Suk Jin Hong , Richik Niloy Ghosh
CPC classification number: G01N33/5014 , G01N33/5067 , G01N2500/10 , G06F19/704 , G06K9/00127 , G06T7/0012 , G06T2207/10056 , G06T2207/30024
Abstract: A method of predicting cell toxicity of a compound includes imaging cells positioned within a plurality of containers to obtain imaged cellular targets, each container being treated with a different concentration of the compound, the imaging being performed using a quantitative high-content cell imaging system; quantitatively measuring the imaged cellular targets to detect changes in multiple cellular targets associated with cytotoxicity of the compound; and analyzing measurements obtained from the measured imaged cellular targets over a range of compound concentrations to determine the toxicity of the compound.
Abstract translation: 预测化合物细胞毒性的方法包括：置于多个容器内的成像细胞，以获得成像的细胞靶标，每个容器用不同浓度的化合物处理，使用定量的高含量细胞成像系统进行成像; 定量测量成像的细胞靶标以检测与化合物的细胞毒性相关的多个细胞靶标的变化; 并且在化合物浓度的范围内分析从测量的成像细胞靶标获得的测量值，以确定化合物的毒性。
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公开(公告)号：US20030104479A1
公开(公告)日：2003-06-05
申请号：US10211088
申请日：2002-08-01
Applicant: Cellomics, Inc.
Inventor： Gary Bright , Daniel Rajadavid Premkumar , Yih-Tai Chen
IPC: G01N033/53 , C07H021/04 , C12P021/04 , C12P021/02 , C12N005/06 , C07K016/46
CPC classification number: C12N15/62 , C07K2319/09 , C07K2319/095 , C07K2319/41 , C07K2319/60 , C07K2319/70
Abstract: The present invention provides novel recombinant fusion proteins for detecting binding of a molecule of interest containing a detection domain, a first and optionally a second localization domain, and a binding domain. The invention also provides recombinant nucleic acid molecules and recombinant expression vectors encoding these novel fusion proteins, genetically engineered host cells containing these expression vectors, and kits for the use of these fusion proteins, nucleic acid molecules, expression vectors, and host cells. Additionally, the present invention provides methods for identifying compounds that alter the binding of a molecule of interest in a cell.
Abstract translation: 本发明提供用于检测包含检测结构域，第一和任选的第二定位结构域和结合结构域的感兴趣分子的结合的新型重组融合蛋白。 本发明还提供重组核酸分子和编码这些新型融合蛋白的重组表达载体，含有这些表达载体的经遗传工程改造的宿主细胞，以及使用这些融合蛋白，核酸分子，表达载体和宿主细胞的试剂盒。 另外，本发明提供了鉴定化合物的方法，所述化合物改变细胞中感兴趣的分子的结合。
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14.发明申请公开(公告)号：US20030059093A1
公开(公告)日：2003-03-27
申请号：US10106589
申请日：2002-03-25
Applicant: Cellomics, Inc.
Inventor： Gustavo Rosania , Paul Sammak , Richard Rubin
IPC: G06K009/00
CPC classification number: G06K9/4647 , G06K9/00127 , G06K9/50 , G06T7/0012 , G06T7/42 , G06T7/62 , G06T7/66 , G06T2207/20048 , G06T2207/30004 , G06T2207/30024
Abstract: The present invention provides automated methods and associated software for determining the organization of a component of interest in individual cells by determining the amount or distribution of the cellular component of interest as a function of position relative to a reference component in the individual cells.
Abstract translation: 本发明提供了自动化方法和相关软件，用于通过根据相对于各个细胞中的参考成分的位置的函数确定感兴趣的细胞成分的量或分布来确定个体细胞中感兴趣的成分的组织。
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公开(公告)号：US20190154652A1
公开(公告)日：2019-05-23
申请号：US16259072
申请日：2019-01-28
Applicant: CELLOMICS, INC.
Inventor： Richik Niloy Ghosh , Dirk John VandenBerg, III , Keith Rao Heffley , Monica Jo Tomaszewski , Jeffrey Robert Haskins
CPC classification number: G01N33/487 , F21Y2105/10 , F21Y2105/14 , G01N21/253 , G01N21/255 , G01N21/31 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N21/6486 , G01N35/00029 , G01N2021/6439 , G01N2021/6491 , G01N2035/00158 , G01N2201/0627 , G01N2201/0631 , G01N2201/064 , G02B5/0278 , G02B5/0294 , G02B21/16 , G02B21/367
Abstract: A system and method that images biological samples and uses chromophores to analyze the imaged samples. The chromophore analysis can be done by itself or in conjunction with fluorophore analysis in High Content Imaging systems. To perform chromophore analysis the biological samples can be labeled with different chromophores and imaged using transmitted light that is at least partially absorbed by the chromophores. To also perform fluorophore analysis the samples can also be labeled with fluorophores that are excited by excitation light. The chromophore analysis and fluorophore analysis can be performed separately or concurrently using a High Content Imaging system. The system provides the expanded capability by illuminating the chromophore-labeled samples with transmitted light of different wavelengths and automatically detecting the images which represent the differential absorption of the colored lights by the sample.
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Patent Agency Ranking
公开(公告)号：US20160041380A1
公开(公告)日：2016-02-11
申请号：US14812874
申请日：2015-07-29
Applicant: CELLOMICS, INC.
Inventor： Ronald Albert Kuhn , Kim Anthony Ippolito , Dirk John Vandenberg, III
CPC classification number: G02B21/365 , G02B7/28 , G02B7/36 , G02B21/0088 , G02B21/16 , G02B21/244 , G02B21/245 , G02B21/361 , G02B21/367 , H04N5/23212
Abstract: Accurate, reliable, and robust laser-based autofocus solutions are presented for through-the-lens microscope applications using slides or micro-titer plates. The laser-based autofocus solutions solve many of the problems that have arisen due to multiple reflective surfaces at varying distances relative to a sample of interest. The laser-based autofocus solutions provide a unique solution to resolve the ambiguity caused by these multiple reflective surfaces by using an image-based approach.
Abstract translation: 为使用幻灯片或微滴定板的透镜显微镜应用提出了准确，可靠和强大的基于激光的自动对焦解决方案。 基于激光的自动对焦解决方案解决了由于多个反射表面相对于感兴趣的样品而在不同距离处产生的许多问题。 基于激光的自动对焦解决方案提供了一种独特的解决方案，可以通过使用基于图像的方法来解决由这些多个反射面引起的模糊性。
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公开(公告)号：US20150323462A1
公开(公告)日：2015-11-12
申请号：US14709239
申请日：2015-05-11
Applicant: CELLOMICS, INC.
Inventor： Richik Niloy Ghosh , Dirk John VandenBerg, III , Keith Rao Heffley , Monica Jo Tomaszewski , Jeffrey Robert Haskins
IPC: G01N21/64 , G01N33/487 , G02B5/02
CPC classification number: G01N33/487 , F21Y2105/10 , F21Y2105/14 , G01N21/253 , G01N21/255 , G01N21/31 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N21/6486 , G01N35/00029 , G01N2021/6439 , G01N2021/6491 , G01N2035/00158 , G01N2201/0627 , G02B5/0278 , G02B5/0294 , G02B21/16 , G02B21/367
Abstract: A system and method that images biological samples and uses chromophores to analyze the imaged samples. The chromophore analysis can be done by itself or in conjunction with fluorophore analysis in High Content Imaging systems. To perform chromophore analysis the biological samples can be labeled with different chromophores and imaged using transmitted light that is at least partially absorbed by the chromophores. To also perform fluorophore analysis the samples can also be labeled with fluorophores that are excited by excitation light. The chromophore analysis and fluorophore analysis can be performed separately or concurrently using a High Content Imaging system. The system provides the expanded capability by illuminating the chromophore-labeled samples with transmitted light of different wavelengths and automatically detecting the images which represent the differential absorption of the colored lights by the sample.
Abstract translation: 对生物样品进行成像和使用发色团分析成像样品的系统和方法。 发色团分析可以自己完成，也可以与高分辨率成像系统中的荧光团分析相结合。 为了进行发色团分析，可以用不同的发色团标记生物样品，并使用至少部分被发色团吸收的透射光成像。 为了进行荧光团分析，样品也可以用被激发光激发的荧光团标记。 发色团分析和荧光团分析可以使用高分辨率成像系统单独或同时进行。 该系统通过用不同波长的透射光照射发色团标记的样品并自动检测表示样品对着色光的差分吸收的图像来提供扩展的能力。
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公开(公告)号：US09075228B2
公开(公告)日：2015-07-07
申请号：US13734748
申请日：2013-01-04
Applicant: Cellomics, Inc.
Inventor： Dirk John Vandenberg, III , Keith R. Heffley
CPC classification number: G02B7/003 , G02B21/00 , G02B26/008 , Y10T29/49817 , Y10T29/49826
Abstract: A filter wheel assembly includes a filter wheel and an optical assembly. The filter wheel includes a plurality of viewing openings. The optical assembly includes an optical element secured within an aperture of a housing. The optical assembly is securable to the filter wheel at a viewing opening by a magnetic force. An optical apparatus includes an optical assembly receiver and first and second optical assemblies. The first optical assembly is securable to the optical assembly receiver and the second optical assembly is securable to the first optical assembly by a magnetic force to align the optical assembly receiver and the optical assemblies. Methods of aligning apertures of optical assemblies with viewing openings of a filter wheel or an optical assembly receiver and securing the optical assemblies to the filter wheel or the optical assembly receiver using a magnetic force.
Abstract translation: 滤光轮组件包括滤光轮和光学组件。 滤光轮包括多个观察孔。 光学组件包括固定在壳体的孔内的光学元件。 光学组件通过磁力固定在观察开口处的滤光轮上。 光学装置包括光学组件接收器和第一和第二光学组件。 第一光学组件可固定到光学组件接收器，并且第二光学组件通过磁力固定到第一光学组件，以对准光学组件接收器和光学组件。 将光学组件的孔径与滤光轮或光学组件接收器的观察孔对准的方法，并使用磁力将光学组件固定到滤光轮或光学组件接收器。
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公开(公告)号：US20130105320A1
公开(公告)日：2013-05-02
申请号：US13658263
申请日：2012-10-23
Applicant: Cellomics, Inc.
Inventor： Brent A. Samson , Chandrasekaran Vasudevan , Keith R. Heffley , Joseph M. Zock , Dirk John Vandenberg, III
IPC: B01L9/00 , G01N27/26 , G01N27/447 , G01N27/453
CPC classification number: G01N27/44756 , B01L3/50 , B01L3/5085 , B01L3/50853 , B01L9/00 , G01N27/453
Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.
Abstract translation: 用于彗星测定的高含量筛选的载片架组件和方法。 滑动支架组件具有内表面，并且构造成容纳在其顶表面上容纳多个生物细胞的载片。 当滑块固定在滑块保持器组件内时，滑块保持器组件的内表面和滑块的顶表面组合形成具有开放顶端的空腔。 空腔是水密的并且构造成在其中保持液体。 使用设置在载玻片保持器组件内的滑块进行彗星测定的方法。 该方法包括在载玻片保持固定在载玻片保持器组件内时，在载玻片上进行细胞的包封，裂解，电泳，染色和成像。
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20.发明申请Synthetic DNA encoding an orange seapen-derived green fluorescent protein with codon preference of mammalian expression systems and biosensors 有权编码橙色海绵衍生的绿色荧光蛋白的合成DNA，具有哺乳动物表达系统和生物传感器的密码子偏好公开(公告)号：US20040265893A1
公开(公告)日：2004-12-30
申请号：US10891302
申请日：2004-07-14
Applicant: Cellomics, Inc.
Inventor： Yih-Tai Chen , Longguang Cao
IPC: C12Q001/68 , C07H021/04 , C07K014/435
CPC classification number: C07K14/43595
Abstract: Synthetic versions of a full length and termini truncated humanized green fluorescent protein based on Ptilosarcus gurneyi are disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild type Ptilosarcus gurneyi which has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons of Ptilosarcus gurneyi fluorescent protein. The humanized Ptilosarcus gurneyi green fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.
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