公开(公告)号：US5995645A

公开(公告)日：1999-11-30

申请号：US984990

申请日：1997-12-04

Applicant: Dirk C. Soenksen ,  George McNamara ,  Yuval Garini ,  Nir Katzir

Inventor： Dirk C. Soenksen ,  George McNamara ,  Yuval Garini ,  Nir Katzir

IPC: G01J3/46 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01J5/10 ,  G01N1/30 ,  G01N21/27 ,  G01N21/64 ,  G01N21/78 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76 ,  G06T1/00 ,  G01N33/48 ,  G01N33/574

CPC classification number: G01N21/6458 ,  G01B11/0675 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J5/10 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/6441

Abstract: A method for cancer cell detection including the steps of (a) staining an analyzed sample with at least first and second dyes, the dyes being selected such that the first dye better adheres to normal cells whereas the second dye better adheres to cancer cells; (b) spectrally imaging the sample through an optical device being optically connected to an imaging spectrometer thereby obtaining a spectrum of each pixel of the sample; (c) based on the spectra, evaluating concentrations of the first and second dyes for each of the pixels; and (d) based on the concentrations detecting the presence of cancer cells in the sample.

Abstract translation: 一种用于癌细胞检测的方法，包括以下步骤：（a）用至少第一和第二染料对分析的样品进行染色，所述染料选择为使得第一染料更好地附着于正常细胞，而第二染料更好地附着于癌细胞; （b）通过光学连接到成像光谱仪的光学装置对样品进行光谱成像，从而获得样品的每个像素的光谱; （c）基于光谱，评估每个像素的第一和第二染料的浓度; 和（d）基于检测样品中癌细胞存在的浓度。

公开(公告)号：US5906919A

公开(公告)日：1999-05-25

申请号：US962845

申请日：1997-11-03

Applicant: Yuval Garini ,  Nir Katzir ,  David Wine ,  Dario Cabib

Inventor： Yuval Garini ,  Nir Katzir ,  David Wine ,  Dario Cabib

IPC: G01N33/48 ,  C12M1/00 ,  C12M3/00 ,  C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N1/28 ,  G01N21/64 ,  G01N21/75 ,  G01N33/483 ,  G01N33/50 ,  G01N33/52 ,  G01N33/569 ,  G01N33/58 ,  G01N37/00 ,  G02B21/00 ,  G02B21/16 ,  G06K9/00 ,  G06K9/76

CPC classification number: G01J3/12 ,  C12Q1/6816 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6423 ,  G01N2021/6441

Abstract: A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

Abstract translation: 一种用于分析L染色体或细胞染色体部分的L个内部参照载体的方法，L染色体或染色体部分用K个不同的荧光团或其组合进行染色，其中K个基本染色体或L个染色体的染色体部分 或染色体的部分各自仅涂覆有K个不同荧光团中的一个，而L个染色体的其他LK或染色体部分各自用不同的K个不同荧光团的组合进行涂布，该方法包括以下步骤：（a） 使用多频带收集装置测量每个L染色体或染色体部分的每个像素的第一个载体; （b）识别属于K个基本染色体或染色体部分的像素，并将该像素定义为基本像素，以获得基本像素的K个基本类别; （c）使用来自K个基本类别的至少一个基本像素来获得K个基本向量，所述K个基本向量是K个内部参考向量; （d）使用K个基本载体来识别属于其他L-K染色体或染色体部分的像素; 和（e）使用属于其他L-K染色体或染色体部分的像素来计算其他L-K内部参考矢量，从而找到所有L个内部参考矢量。 使用上述用于寻找L个内部参考向量的方法，并且使用L个参考向量将每个像素分类为L个分类等级之一来分类L染色体或染色体部分的方法。 而且，呈现彩色染色体的图像。

公开(公告)号：US07801682B2

公开(公告)日：2010-09-21

申请号：US10546152

申请日：2004-02-23

Applicant: Sabine Mai ,  Tony Chih Yuan Chuang ,  Sharareh Moshir ,  Yuval Garini

Inventor： Sabine Mai ,  Tony Chih Yuan Chuang ,  Sharareh Moshir ,  Yuval Garini

IPC: G06F19/00 ,  G06K9/20 ,  G06K9/36 ,  G06K9/00

CPC classification number: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2565/601

Abstract: The present invention relates to a method of detecting and monitoring genomic instability in a cell using three-dimensional analysis to assess telomeric and/or chromosomal organization. In addition, the invention relates to a method and system for characterizing the 3D organization of telomeres and/or chromosomes. The system includes an input module for inputting image data of the 3D organization of telomeres and/or chromosomes and a characteristic module for finding a parameter of the 3D organization. The invention also relates to the use of the three-dimensional analysis to detect, diagnose or monitor disease, particularly proliferative diseases such as cancer.

Abstract translation: 本发明涉及使用三维分析来检测和监测细胞中基因组不稳定性以评估端粒和/或染色体组织的方法。 此外，本发明涉及用于表征端粒和/或染色体的3D组织的方法和系统。 该系统包括用于输入端粒和/或染色体的3D组织的图像数据的输入模块和用于找到3D组织的参数的特征模块。 本发明还涉及三维分析用于检测，诊断或监测疾病，特别是增殖性疾病如癌症的用途。

公开(公告)号：US20100143903A1

公开(公告)日：2010-06-10

申请号：US12443781

申请日：2007-09-28

Applicant: Sabine Mai ,  Yuval Garini ,  Rahul Sarkar ,  Bartholomeus J. Vermolen

Inventor： Sabine Mai ,  Yuval Garini ,  Rahul Sarkar ,  Bartholomeus J. Vermolen

IPC: C12Q1/68

CPC classification number: G06F19/345 ,  C12Q1/6886 ,  G01N15/1475 ,  G01N33/5035 ,  G01N33/5091 ,  G01N33/574 ,  G01N33/57426 ,  G06K9/0014 ,  G16H50/20

Abstract: The present application relates to a method of detecting and monitoring cancer or precancer in a cell using three-dimensional analysis to assess centromere organization. In addition, the application relates to a method and system for characterizing the 3D organization of centromeres.

Abstract translation: 本申请涉及使用三维分析来检测和监测细胞中的癌症或癌前期的方法以评估着丝粒组织。 此外，该申请涉及用于表征着丝粒的3D组织的方法和系统。

公开(公告)号：US20070031831A1

公开(公告)日：2007-02-08

申请号：US10546152

申请日：2004-02-23

Applicant: Sabine Mai ,  Tony Chuang ,  Sharareh Moshir ,  Yuval Garini

Inventor： Sabine Mai ,  Tony Chuang ,  Sharareh Moshir ,  Yuval Garini

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2565/601

Abstract: The present invention relates to a method of detecting and monitoring genomic instability in a cell using three-dimensional analysis to assess telomeric and/or chromosomal organization. In addition, the invention relates to a method and system for characterizing the 3D organization of telomeres and/or chromosomes. The system includes an input module for inputting image data of the 3D organization of telomeres and/or chromosomes and a characteristic module for finding a parameter of the 3D organization. The invention also relates to the use of the three-dimensional analysis to detect, diagnose or monitor disease, particularly proliferative diseases such as cancer.

Abstract translation: 本发明涉及使用三维分析来检测和监测细胞中基因组不稳定性以评估端粒和/或染色体组织的方法。 此外，本发明涉及用于表征端粒和/或染色体的3D组织的方法和系统。 该系统包括用于输入端粒和/或染色体的3D组织的图像数据的输入模块和用于找到3D组织的参数的特征模块。 本发明还涉及三维分析用于检测，诊断或监测疾病，特别是增殖性疾病如癌症的用途。

公开(公告)号：US06552794B2

公开(公告)日：2003-04-22

申请号：US09824684

申请日：2001-04-04

Applicant: Yuval Garini

Inventor： Yuval Garini

IPC: G01N2155

CPC classification number: G06K19/06046 ,  G06K7/10801

Abstract: A light reflecting article is disclosed. The light reflecting article comprises a sample carrying article layered with a light reflecting layer. The light reflecting layer serves for allowing an optical collection and detection system to collect both luminescent light emitted from a sample positioned on the light reflecting article in a direction of the optical collection and detection system, as well as luminescent light emitted from the sample in a direction away from the optical collection and detection system and reflected in the direction of the optical collection and detection system via the light reflecting layer, thereby increasing a sensitivity of luminescent light detection.

公开(公告)号：US5936731A

公开(公告)日：1999-08-10

申请号：US575191

申请日：1995-12-20

Applicant: Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

Inventor： Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

IPC: G01B9/02 ,  A61B3/12 ,  A61B5/00 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

CPC classification number: G01N21/6458 ,  A61B3/12 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01B11/0675 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  A61B3/1241 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A fluorescent in situ hybridization method comprising the steps of (a) providing a cell nuclei having chromosomes hybridized with at least one nucleic acid probe including at least one nucleic acid molecule labeled with at least one fluorophore; (b) viewing the cell nuclei through a fluorescence microscope optically connected to an imaging spectrometer for obtaining a spectrum of each pixel of the cell nuclei by (i) collecting incident collimated light simultaneously from all pixels of the cell nuclei; (ii) passing the incident collimated light through an interferometer system so that the light is first split into two coherent beams and then recombine to interfere and form an exiting light beam; (iii) focusing the exiting light beam on a detector having an array of detector elements, so that at each instant each of the elements is the image of one and always the same pixel for the entire duration of the measurement and so that each of the elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths; (iv) rotating or translating one or more of the elements of the interferometer, so that the optical path difference is scanned simultaneously for all the pixels of the cell nuclei; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

Abstract translation: 荧光原位杂交方法包括以下步骤：（a）提供具有染色体的细胞核，所述染色体与至少一种核酸探针杂交，所述核酸探针包括至少一个用至少一个荧光团标记的核酸分子; （b）通过光学连接到成像光谱仪的荧光显微镜观察细胞核，通过（i）从细胞核的所有像素同时收集入射准直光，以获得细胞核的每个像素的光谱; （ii）使入射的准直光通过干涉仪系统，使得光首先分裂成两个相干光束，然后重组以干涉并形成出射的光束; （iii）将出射的光束聚焦在具有检测器元件阵列的检测器上，使得在每个时刻，在整个测量持续时间内，每个元件是一个并且总是相同像素的图像，并且使得每个 元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合; （iv）旋转或平移干涉仪的一个或多个元件，使得对于细胞核的所有像素同时扫描光程差; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

公开(公告)号：US5817462A

公开(公告)日：1998-10-06

申请号：US635820

申请日：1996-04-22

Applicant: Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald ,  Thomas Ried ,  Dirk G. Soenksen

Inventor： Yuval Garini ,  Dario Cabib ,  Robert A. Buckwald ,  Thomas Ried ,  Dirk G. Soenksen

IPC: C12N15/09 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/16 ,  G06K9/00 ,  G06K9/76 ,  C07H23/00 ,  G02B21/00

CPC classification number: G01N21/6452 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.