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    MICROFLUIDIC NUCLEIC ACID ANALYSIS
    11.
    发明申请
    MICROFLUIDIC NUCLEIC ACID ANALYSIS 审中-公开
    微流核酸分析

    公开(公告)号：US20150238960A1

    公开(公告)日：2015-08-27

    申请号：US14494284

    申请日：2014-09-23

    Applicant: California Institute of Technology

    Inventor： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    IPC: B01L3/00 ,  C12Q1/68

    CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    Abstract translation: 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。 随后洗脱纯化的核酸之后可以连接和细胞转化，全部在相同的微流体芯片内。 在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

    Apparatus for preparing cDNA libraries from single cells
    14.
    发明授权
    Apparatus for preparing cDNA libraries from single cells 有权

    公开(公告)号：US10328428B2

    公开(公告)日：2019-06-25

    申请号：US15406451

    申请日：2017-01-13

    Applicant: California Institute of Technology

    Inventor： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    IPC: C12Q1/68 ,  B01L3/00 ,  C12Q1/6806

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    APPARATUS AND METHODS FOR CONDUCTING ASSAYS AND HIGH THROUGHPUT SCREENING
    17.
    发明申请
    APPARATUS AND METHODS FOR CONDUCTING ASSAYS AND HIGH THROUGHPUT SCREENING 审中-公开
    用于导电测试和高通量筛选的装置和方法

    公开(公告)号：US20160123958A1

    公开(公告)日：2016-05-05

    申请号：US14928631

    申请日：2015-10-30

    Applicant: California Institute of Technology

    Inventor： Stephen R. Quake ,  Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer

    IPC: G01N33/50 ,  B01L3/00

    Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

    Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。

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