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公开(公告)号：US20150031116A1
公开(公告)日：2015-01-29
申请号：US14197451
申请日：2014-03-05
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Stephen R. Quake , Hou-Pu Chou
IPC: B01L3/00
CPC classification number: B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
Abstract translation: 本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。 本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病。 与杂交多核苷酸或抗原/抗体复合物相关的报道。 在根据本发明的微制造装置中，分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。 这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。 杂交探针可以固定在形成一部分或暴露于形成闭环的装置的通道或通道的基底上，用于循环样品以主动接触互补探针。 根据本发明的通用芯片可以不仅用DNA而且可以与其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子一起制备。
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公开(公告)号：US20150276089A1
公开(公告)日：2015-10-01
申请号：US14188664
申请日：2014-02-24
Applicant: California Institute of Technology
Inventor： Marc Alexander Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Markus Enzelberger , Mark L. Adams , Carl L. Hansen
IPC: F16K99/00
CPC classification number: B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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公开(公告)号：US10509018B2
公开(公告)日：2019-12-17
申请号：US14928631
申请日：2015-10-30
Applicant: California Institute of Technology
Inventor： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
IPC: G01N15/06 , G01N33/00 , G01N33/48 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/08 , B01F5/06 , B01F11/00 , B01F13/00 , B01F13/10 , B01L3/00 , F04B43/04 , F16K99/00 , G01N27/447 , G01N33/50 , C12M3/06 , C12M1/34 , G01N33/569 , B01F7/00
Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
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4.发明申请公开(公告)号：US20160123958A1
公开(公告)日：2016-05-05
申请号：US14928631
申请日：2015-10-30
Applicant: California Institute of Technology
Inventor： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。
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5.发明授权公开(公告)号：US09176137B2
公开(公告)日：2015-11-03
申请号：US14216740
申请日：2014-03-17
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N33/569 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/08 , B01F5/06 , B01F11/00 , B01F13/00 , B01F13/10 , F04B43/04 , F16K99/00 , G01N27/447 , G01N33/50 , C12M3/06 , C12M1/34 , B01F7/00 , B01L3/00
CPC classification number: G01N33/00 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/081 , B01D63/088 , B01D2313/08 , B01D2313/90 , B01F5/0646 , B01F5/0647 , B01F7/00 , B01F11/0071 , B01F13/0059 , B01F13/1013 , B01F13/1022 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L2200/0631 , B01L2200/10 , B01L2300/0627 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , B01L2400/082 , C12M23/16 , C12M41/46 , F04B43/043 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N15/06 , G01N27/44743 , G01N27/44791 , G01N33/5005 , G01N33/5008 , G01N33/56966 , Y10T436/11 , Y10T436/117497 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。
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Patent Agency Ranking
公开(公告)号：US20160339423A1
公开(公告)日：2016-11-24
申请号：US15078884
申请日：2016-03-23
Applicant: California Institute of Technology
Inventor： Stephen R. Quake , Hou-Pu Chou
IPC: B01L3/00
CPC classification number: B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
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公开(公告)号：US20150183633A1
公开(公告)日：2015-07-02
申请号：US14503252
申请日：2014-09-30
Applicant: California Institute of Technology
Inventor： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
IPC: B81B3/00
CPC classification number: B32B3/00 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B81B3/0032 , B81B3/0051 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6874 , F04B43/043 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/2224 , Y10T137/87716 , Y10T137/87804 , Y10T137/87812 , Y10T137/87893 , Y10T156/10 , Y10T156/1002 , Y10T156/1064 , Y10T428/24479 , Y10T428/2457 , Y10T428/24612 , Y10T428/24744 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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公开(公告)号：US20140322489A1
公开(公告)日：2014-10-30
申请号：US14218872
申请日：2014-03-18
Applicant: California Institute of Technology
Inventor： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
IPC: F16K13/00
CPC classification number: B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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公开(公告)号：US10155250B2
公开(公告)日：2018-12-18
申请号：US15173262
申请日：2016-06-03
Applicant: California Institute of Technology
Inventor： Hou-Pu Chou , Stephen R. Quake
IPC: G01N1/10 , B01L3/00 , B05D3/04 , F15C1/06 , F15C3/00 , C30B29/54 , B65B31/00 , B01L9/00 , B81C1/00 , C12Q1/6874 , F04B43/04 , F15C5/00 , F16K99/00 , C12Q1/6832 , B01L7/00 , B32B37/10
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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公开(公告)号：US20170001195A1
公开(公告)日：2017-01-05
申请号：US15173262
申请日：2016-06-03
Applicant: California Institute of Technology
Inventor： Marc Alexander Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Markus Enzelberger , Mark L. Adams , Carl L. Hansen
CPC classification number: B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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