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    HEPARIN AFFINITY TAG AND APPLICATIONS THEREOF
    36.
    发明申请
    HEPARIN AFFINITY TAG AND APPLICATIONS THEREOF 有权
    HEPARIN AFFINITY标签及其应用

    公开(公告)号：US20160145597A1

    公开(公告)日：2016-05-26

    申请号：US14378230

    申请日：2014-01-21

    Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS

    Inventor： Suresh Kumar Thallapuranam ,  Srinivas Jayanthi ,  Jacqueline Morris ,  Alicia Brown ,  David McNabb ,  Ralph Henry

    IPC: C12N9/64 ,  C07K14/00

    CPC classification number: C07K1/22 ,  B01D15/206 ,  B01D15/22 ,  B01D15/3809 ,  B01D69/08 ,  B01J20/28078 ,  B01J20/286 ,  C07K14/00 ,  C07K2319/20 ,  C07K2319/50 ,  C12N9/6424 ,  C12Y304/21043

    Abstract: In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.

    Abstract translation: 一方面，本文描述了用于重组蛋白质纯化的亲和标签，其在一些实施方案中可以减轻或克服先前亲和标签系统的缺点。 在一些实施方案中，例如，本文所述的亲和标签允许用简化的溶液系统（例如碱金属盐溶液）有效洗脱所需重组蛋白。 本文所述的亲和标签包含包含BXXXBXX的重复氨基酸单元的氨基酸序列，其中B是选自组氨酸，赖氨酸和精氨酸的氨基酸，X是选自氨基酸 除了组氨酸，赖氨酸和精氨酸。

    NOVEL GRAFT POLYMER, TEMPERATURE-RESPONSIVE SUBSTRATE FOR CELL CULTURE USING THE SAME AND PRODUCTION METHOD THEREFOR, AS WELL AS LIQUID CHROMATOGRAPHIC CARRIER HAVING THE NOVEL GRAFT POLYMER IMMOBILIZED THEREON AND LIQUID CHROMATOGRAPHIC METHOD USING THE SAME
    38.
    发明申请
    NOVEL GRAFT POLYMER, TEMPERATURE-RESPONSIVE SUBSTRATE FOR CELL CULTURE USING THE SAME AND PRODUCTION METHOD THEREFOR, AS WELL AS LIQUID CHROMATOGRAPHIC CARRIER HAVING THE NOVEL GRAFT POLYMER IMMOBILIZED THEREON AND LIQUID CHROMATOGRAPHIC METHOD USING THE SAME 审中-公开
    新型聚合物，使用其的细胞培养物的温度响应性基质及其生产方法，以及具有使用其的新型聚酰胺聚合物的液体色谱载体和液相色谱方法

    公开(公告)号：US20160115441A1

    公开(公告)日：2016-04-28

    申请号：US14771068

    申请日：2014-02-28

    Applicant: Hideaki SAKAI

    Inventor： Hideaki SAKAI ,  Masa-aki KAKIMOTO ,  Yu SUDO

    IPC: C12N5/00 ,  C08F257/02

    CPC classification number: C12N5/0068 ,  B01D15/305 ,  B01D15/327 ,  B01D15/3876 ,  B01J20/264 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3278 ,  C08F257/02 ,  C08F293/005 ,  C08F299/00 ,  C08F2438/03 ,  C08L51/003 ,  C08L101/04 ,  C08L2203/02 ,  C12M23/20 ,  C12N2533/30 ,  C12N2539/10

    Abstract: By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

    Abstract translation: 通过使用包含具有苯乙烯骨架的树枝状聚合物和接枝到其末端的亲水性聚合物的接枝聚合物，具有细胞培养的温度敏感性底物，其具有允许细胞高效培养的细胞培养的温度敏感性表面，以及 这可以方便地制备通过简单地改变基材表面的温度在短时间内和高效率地剥离培养的细胞。 如果使用用于细胞培养的温度敏感性底物，则可以高效地培养从各种组织获得的细胞。 如果使用这种培养方法，培养的细胞可以在短时间内以高效率完整地剥离。 此外，通过使用该接枝聚合物，也可以通过简单地改变色谱载体的温度来分离多种肽和蛋白质。 这允许方便的分离程序并提高分离操作的效率。 此外，树枝状聚合物本身的立构规整性可以用于基于其分子结构的差异来分离溶质。

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