公开(公告)号：US20060032816A1

公开(公告)日：2006-02-16

申请号：US10915735

申请日：2004-08-10

Applicant: Richard Marcus ,  Rayman Stanelle

Inventor： Richard Marcus ,  Rayman Stanelle

IPC: B01D11/04

CPC classification number: B01D15/08 ,  B01D15/22 ,  B01J20/261 ,  B01J20/262 ,  B01J20/28014 ,  B01J20/28023 ,  B01J20/28042 ,  B01J20/28095 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/62 ,  B01J2220/82 ,  G01N30/6043 ,  G01N30/6078 ,  G01N30/6091 ,  G01N2030/528

Abstract: Monolithic cartridges including a plurality of nominally aligned polymer fibers can be used as stationary phase materials for liquid chromatography separations. Bundles of fibers are packed together so as to form capillary channels between the fibers. Different polymer compositions permit the “chemical tuning” of the separation process. The fibers can be physically or chemically bonded at spaced locations throughout the cartridge or can be packed together under pressure by use of an encasing wrap to form the capillary channels. Use of fibers allows a wide range of liquid flow rates with very low backpressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, waste remediation/immobilization, extraction of selected organic molecules/ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.

Abstract translation: 包括多个名义上排列的聚合物纤维的单片墨盒可用作液相色谱分离的固定相材料。 纤维束被包装在一起，以在纤维之间形成毛细通道。 不同的聚合物组合物允许分离过程的“化学调节”。 纤维可以在整个药筒的间隔位置物理地或化学地结合，或者可以通过使用包裹包装在压力下包装在一起以形成毛细通道。 纤维的使用允许具有非常低的背压的宽范围的液体流速。 应用于HPLC，cap-LC，预分离分离，分析分离，废物修复/固定，从溶液中萃取选定的有机分子/离子，液体流净化（工艺废物，饮用水，纯溶剂），细胞的选择性提取 生长介质中的物质和细菌，以及细胞和细菌的固定化。

公开(公告)号：US20050211616A1

公开(公告)日：2005-09-29

申请号：US11050004

申请日：2005-02-03

Applicant: Anthony DiLeo ,  Justin McCue ,  Wilson Moya ,  Igor Quinones-Garcia ,  Neil Soice ,  Volkmar Thom ,  Sarah Yuan

Inventor： Anthony DiLeo ,  Justin McCue ,  Wilson Moya ,  Igor Quinones-Garcia ,  Neil Soice ,  Volkmar Thom ,  Sarah Yuan

IPC: B01D15/08 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32

CPC classification number: B01J20/286 ,  B01J20/262 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28042 ,  B01J20/28078 ,  B01J20/28085 ,  B01J20/3035 ,  B01J20/305 ,  B01J20/321 ,  B01J20/3251 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J2220/62 ,  B01J2220/82

Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be crosslinked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.

Abstract translation: 能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附性涂层的多孔基材，其包含基材孔隙体积的约1至约80％。 所得到的底物具有良好的对流和扩散流量和容量。 底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

公开(公告)号：US06946070B2

公开(公告)日：2005-09-20

申请号：US09808732

申请日：2001-03-14

Applicant: Richard F. Hammen ,  John P. Hammen

Inventor： Richard F. Hammen ,  John P. Hammen

IPC: B01J20/285 ,  B01D15/00 ,  B01D15/08 ,  B01D15/36 ,  B01J19/00 ,  B01J20/28 ,  B01J20/281 ,  B01J39/16 ,  B01J41/12 ,  B01J47/00 ,  C07B61/00 ,  C40B60/14 ,  G01N30/88

CPC classification number: B01J20/3282 ,  B01D15/00 ,  B01D15/08 ,  B01D15/361 ,  B01J19/0046 ,  B01J20/28014 ,  B01J20/28019 ,  B01J20/28021 ,  B01J20/28023 ,  B01J20/28026 ,  B01J20/28042 ,  B01J20/286 ,  B01J20/3268 ,  B01J20/3272 ,  B01J20/328 ,  B01J39/17 ,  B01J39/26 ,  B01J41/12 ,  B01J41/20 ,  B01J47/018 ,  B01J2219/00286 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/0072 ,  B01J2220/54 ,  B01J2220/82 ,  B01J2220/84 ,  C07B2200/11 ,  C40B40/00 ,  C40B60/14

Abstract: The present invention relates in general to the preparation and use of matrices having solid spaces, interstitial spaces and interstitial polymer networks. In particular, the interstitial polymer networks have utility in chemical and biochemical separations, solid phase synthesis, catalysis of chemical reactions, and immobilized enzyme reactors. The interstitial polymer networks in one embodiment comprise crosslinked polymers suspended in the interstitial spaces from and/or between solid particles. The matrices are characterized by high ligand and functional group density and by reversible high sorptive and binding capacity, and are substantially accompanied by a very low nonspecific adsorption or interaction with molecules such as proteins. Moreover, the matrices of the invention exhibit other characteristics highly desirable in chromatographic and catalytic applications, such as high physical rigidity, high ligand density, chemical stability, high ligand reactivity, and rapid exchange and reaction kinetics.

Abstract translation: 本发明一般涉及具有固体空间，间隙和间质聚合物网络的基质的制备和使用。 特别地，间隙聚合物网络可用于化学和生化分离，固相合成，化学反应催化和固定化酶反应器。 在一个实施方案中，间隙聚合物网络包括悬浮在固体颗粒之间和/或固体颗粒之间的交联聚合物。 基质的特征在于高配体和官能团密度以及可逆的高吸附和结合能力，并且基本上伴随着与诸如蛋白质的分子非常低的非特异性吸附或相互作用。 此外，本发明的基质表现出在色谱和催化应用中非常需要的其它特征，例如高物理刚度，高配体密度，化学稳定性，高配位体反应性和快速交换和反应动力学。

公开(公告)号：US06863820B2

公开(公告)日：2005-03-08

申请号：US10240921

申请日：2001-03-13

Applicant: Karin Cabrera ,  Alexander Kraus ,  Willi Neuroth ,  Dieter Lubda

Inventor： Karin Cabrera ,  Alexander Kraus ,  Willi Neuroth ,  Dieter Lubda

IPC: B01D15/08 ,  B01J20/28 ,  B01J20/281 ,  B01J20/283 ,  C02F1/28 ,  G01N30/00 ,  G01N30/52 ,  G01N30/60 ,  G01N30/88

CPC classification number: B01J20/281 ,  B01J20/28042 ,  B01J2220/54 ,  B01J2220/82 ,  G01N30/60 ,  G01N2030/528

Abstract: The invention relates to monolithic sorbents with a casing of fiber-reinforced plastic. Through the use of fiber-reinforced plastic having a viscosity of between 40 and 100 ml/10 min by the MVI method, the casing can be applied to the moulding leaving just a small dead space and has adequate mechanical stability.

Abstract translation: 本发明涉及具有纤维增强塑料外壳的整体吸附剂。 通过使用通过MVI方法粘度为40-100ml / 10分钟的纤维增强塑料，可以将外壳施加到模制件上，只留下较小的死空间并具有足够的机械稳定性。

公开(公告)号：US20040262229A1

公开(公告)日：2004-12-30

申请号：US10854244

申请日：2004-05-27

Inventor： Yoshio Okamoto ,  Chiyo Yamamoto ,  Chankvetadze Bezhan

IPC: C08B037/00 ,  C02F001/28 ,  B01D015/08

CPC classification number: B01J20/286 ,  B01D15/3833 ,  B01J20/103 ,  B01J20/28042 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3257 ,  B01J20/3263 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/82 ,  B01J2220/84 ,  C07B57/00

Abstract: The present invention provides a separating agent for optical isomers having a porous monolithic inorganic type carrier and polysaccharide or a derivative thereof supported on the monolithic inorganic type carrier, and a separation column for optical isomers in which the separating agent for optical isomers is held in a column tube. According to the invention, the separating agent for optical isomers and the separation column for optical isomers which have high asymmetry recognition ability and can be used particularly at a high flow rate when used for the separation of optical isomers is provided.

Abstract translation: 本发明提供一种用于光学异构体的分离剂，其具有多孔单片无机载体和负载在单片无机载体上的多糖或其衍生物，以及用于光学异构体的分离柱，其中旋光异构体的分离剂保持在 柱管。 根据本发明，提供了用于光学异构体的分离剂和具有高不对称识别能力的光学异构体的分离柱，并且当用于分离光学异构体时可以特别以高流速使用。

公开(公告)号：US20040236083A1

公开(公告)日：2004-11-25

申请号：US10835514

申请日：2004-04-29

Inventor： Sharon Ann Libert ,  Victor Sisto Lusvardi ,  Timothy Tucker Norton

IPC: C07K001/12

CPC classification number: B01D15/22 ,  B01D15/34 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/28033 ,  B01J20/28042 ,  B01J20/285 ,  B01J2220/82 ,  C07K1/20 ,  G01N30/6052

Abstract: The present invention relates to columns for carrying out separation processes involving protein solutions, wherein at least the interior surface of the column is comprised of fluoropolymer.

Abstract translation: 本发明涉及用于进行涉及蛋白质溶液的分离过程的柱，其中至少柱的内表面由含氟聚合物组成。

公开(公告)号：US06814870B2

公开(公告)日：2004-11-09

申请号：US10658008

申请日：2003-09-09

Applicant: Sheng Zhang ,  Xian Huang ,  Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser

Inventor： Sheng Zhang ,  Xian Huang ,  Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser

IPC: B01D1508

CPC classification number: G01N30/6095 ,  B01D15/3804 ,  B01J20/28042 ,  B01J20/285 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3244 ,  B01J20/3251 ,  B01J20/3274 ,  B01J45/00 ,  B01J2220/52 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/82 ,  G01N30/463 ,  G01N30/466 ,  G01N30/482 ,  G01N30/7266 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array and a capillary tube or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modem life sciences such as proteomics and drug discover.

Abstract translation: 公开了一种基于微芯片的电喷雾离子化装置和具有亲和吸附剂的柱。 本发明包括微芯片阵列和毛细管，或单独或组合连接。 装置或柱的至少一部分具有固定的亲和吸附剂。 还提供了使用该装置的方法用于亲和捕获生物分子以满足现代生命科学如蛋白质组学和药物发现的需要。

公开(公告)号：US20040138432A1

公开(公告)日：2004-07-15

申请号：US10473415

申请日：2004-03-10

Inventor： Camilla Viklund ,  Knut Irgum

IPC: C07K001/16

CPC classification number: B01J20/285 ,  B01D15/34 ,  B01J20/26 ,  B01J20/261 ,  B01J20/267 ,  B01J20/28042 ,  B01J2220/82 ,  C08F212/08 ,  C08F257/02

Abstract: The present invention provides porous monoliths with high flow permeability that can be produced by polymerising and divinylbenzene in the presence of an initiator, a carboxy-functionalized nitroxide stable free radical and polymeric porogens. The monoliths produced with these stable free radicals functionalized with carboxylic groups feature porosities very different from those produced by polymerizations involving other SFRs, characterized by very large surface areas in combination with relatively large through-pores. The invention also provides a method for producing the monoliths, a column containing the monoliths and an assay method using the column.

Abstract translation: 本发明提供了在引发剂，羧基官能化的氮氧自由基和聚合物致孔剂的存在下可以通过聚合和二乙烯基苯来制备具有高流动性的多孔整料。 用这些用羧基官能化的这些稳定的自由基产生的整料特征在于与通过涉及其它SFR的聚合产生的那些非常不同的孔隙度，其特征在于具有相当大的通孔的非常大的表面积。 本发明还提供了一种生产整料的方法，一种含有整料的柱和使用该柱的测定方法。

公开(公告)号：US20040002081A1

公开(公告)日：2004-01-01

申请号：US10321792

申请日：2002-12-18

Applicant: Boehringer Ingelheim International GmbH and BIA Separations d.o.o.

Inventor： Jochen Urthaler ,  Roman Necina ,  Janez Jancar ,  Ales Podgornik ,  Milos Barut ,  Ales Strancar ,  Mojca Merhar

IPC: C12Q001/68

CPC classification number: C12N15/101 ,  B01D15/322 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  B01J20/282 ,  B01J2220/82

Abstract: The invention relates to a process for isolating and purifying a polynucleotide on a manufacturing scale which uses a chromatographic separation process comprising a combination of two different chromatographic steps selected from hydrophobic interaction chromatography, polar interaction chromatography and anion exchange chromatography. In at least one of the two steps the chromatographic support is a porous monolithic bed. The invention also relates to a chromatographic device and its use for isolating and purifying a polynucleotide of interest, in particular plasmid DNA, on manufacturing scale.

Abstract translation: 本发明涉及一种用于分离和纯化制造规模的多核苷酸的方法，其使用色谱分离方法，其包括选自疏水相互作用层析，极性相互作用层析和阴离子交换层析的两种不同色谱步骤的组合。 在两个步骤中的至少一个步骤中，色谱支持是多孔整体床。 本发明还涉及色谱装置及其在制造规模上分离和纯化目的多核苷酸，特别是质粒DNA的用途。

公开(公告)号：US20030201390A1

公开(公告)日：2003-10-30

申请号：US10405689

申请日：2003-04-01

Inventor： Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser ,  Xian Huang

IPC: H01J049/00 ,  B01D059/44

CPC classification number: G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.