公开(公告)号：US11994453B2

公开(公告)日：2024-05-28

申请号：US17112408

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew Lauber ,  Michael Donegan

IPC: G01N1/40 ,  B01D15/42 ,  B01J20/28 ,  B01J20/281 ,  B01J20/34 ,  G01N30/06 ,  G01N30/16 ,  G01N30/00

CPC classification number: G01N1/405 ,  B01D15/426 ,  B01J20/28052 ,  B01J20/281 ,  B01J20/3475 ,  G01N30/06 ,  G01N30/16 ,  B01J2220/54 ,  G01N2030/009 ,  G01N2030/067

Abstract: The present technology relates to a method for improved recovery of phosphorylated compounds (e.g., phosphorylated glycans). In particular, the present technology utilizes certain chelator additives in a solution to wash and elute the phosphorylated compounds from a solid phase extraction cartridge.

公开(公告)号：US20230323312A1

公开(公告)日：2023-10-12

申请号：US18333718

申请日：2023-06-13

Applicant: The Trustees of the University of Pennsylvania

Inventor： Martin Lock ,  Mauricio Alvira

IPC: C12N7/02 ,  B01J20/281 ,  B01D15/08 ,  C12N7/00 ,  B01J41/13 ,  C12N15/86 ,  B01J47/022 ,  B01D15/38 ,  C12N15/864 ,  G01N21/33

CPC classification number: C12N7/02 ,  B01J20/281 ,  B01D15/08 ,  C12N7/00 ,  B01J41/13 ,  C12N15/86 ,  B01J47/022 ,  B01D15/3804 ,  C12N15/8645 ,  G01N21/33 ,  B01J41/05

Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

公开(公告)号：US11718835B2

公开(公告)日：2023-08-08

申请号：US17328686

申请日：2021-05-24

Applicant: The Trustees of the University of Pennsylvania

Inventor： Martin Lock ,  Mauricio Alvira

IPC: C12N7/02 ,  C12N7/00 ,  B01D15/38 ,  B01J20/281 ,  C12N15/864 ,  G01N21/33 ,  B01J41/05 ,  B01D15/16 ,  B01D15/36 ,  B01J41/20

CPC classification number: C12N7/02 ,  B01D15/3804 ,  B01J20/281 ,  C12N7/00 ,  C12N15/8645 ,  G01N21/33 ,  B01D15/166 ,  B01D15/363 ,  B01J41/05 ,  B01J41/20 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

公开(公告)号：US20190211088A1

公开(公告)日：2019-07-11

申请号：US16310271

申请日：2017-06-09

Applicant: Counterpoint Biomedica LLC

Inventor： Frederick L. Hall ,  Erlinda M. Gordon

IPC: C07K16/18 ,  C07K16/22 ,  A61K47/60 ,  G01N33/50 ,  G01N33/574 ,  A61K49/00 ,  A61P35/00 ,  A61K39/395 ,  A61P31/12 ,  C12N5/09 ,  C07K14/78 ,  A61K38/39 ,  A61M1/36 ,  B01D15/38 ,  B01D15/34 ,  B01J20/281 ,  G01N30/84

CPC classification number: C07K16/18 ,  A61K38/00 ,  A61K38/39 ,  A61K39/3955 ,  A61K47/60 ,  A61K49/0004 ,  A61M1/3687 ,  A61P31/12 ,  A61P35/00 ,  B01D15/34 ,  B01D15/3809 ,  B01J20/281 ,  C07K14/78 ,  C07K16/22 ,  C07K2317/92 ,  C07K2319/21 ,  C07K2319/24 ,  C07K2319/40 ,  C12N5/0693 ,  G01N30/482 ,  G01N30/84 ,  G01N33/5011 ,  G01N33/5091 ,  G01N33/57492 ,  G01N2030/484

Abstract: Provided herein are new compositions and methods to target and deliver agents to pathological areas by utilizing multifunctional compounds. These compounds include three or more domains: (i) a vimentin-binding peptide, (ii) a linker, and (iii) a drug binding, a capturing reagent, or a detectable moiety. These compounds can be used to detect, isolate, and/or treat cancerous cells such as circulating tumor cells.

公开(公告)号：US20190184372A1

公开(公告)日：2019-06-20

申请号：US16327437

申请日：2017-08-10

Applicant: SHOWA DENKO K.K.

Inventor： Junya KATO ,  Hideyuki KONDO ,  Ryuji TAKAHASHI

IPC: B01J20/26 ,  B01J20/285 ,  B01J20/28 ,  G01N30/88 ,  B01J20/281

CPC classification number: B01J20/267 ,  B01D15/00 ,  B01D15/38 ,  B01D15/42 ,  B01J20/26 ,  B01J20/28 ,  B01J20/28004 ,  B01J20/28083 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/86 ,  G01N30/482 ,  G01N30/88 ,  G01N2030/8836

Abstract: Provided is a packing material for HILIC columns for more accurately and more easily performing oligosaccharide analysis by liquid chromatography; an HILIC column which is filled with the packing material for HILIC columns; and a method for analyzing an oligosaccharide with use of this packing material for HILIC columns A packing material for HILIC columns according to the present invention is composed of particles, each of which is obtained by reacting glycidol to a hydroxyl group of a porous cross-linked polymer base material having the hydroxyl group, and which have a hydrophilicity index of 2.30 or more and a surface-pH index of from 0.95 to 1.05.

公开(公告)号：US20180340923A1

公开(公告)日：2018-11-29

申请号：US15602877

申请日：2017-05-23

Applicant: GM Global Technology Operations LLC

Inventor： Justin Pletzke ,  Shaelah M. Reidy

IPC: G01N33/28 ,  B01J20/26 ,  B01J20/281 ,  G01N30/72 ,  G01N30/88 ,  B01D15/38

CPC classification number: G01N33/2835 ,  B01D15/3804 ,  B01J20/262 ,  B01J20/281 ,  B01J2220/54 ,  G01N30/482 ,  G01N30/88 ,  G01N2030/8854 ,  G01N2030/8859

Abstract: A method for characterizing a brake fluid, the method comprising contacting a solvent with the brake fluid to provide a sample; introducing the sample into a first capillary column coated with a first stationary phase and allowing the sample to flow through the first capillary column to produce an effluent stream; and passing the effluent stream through a detector to identify one or more hydrocarbon components and optionally a detectable borate component, wherein the first capillary column is at a temperature effective for a borate ester in the sample, if any, to react with a portion of the solvent to form the detectable borate component.

公开(公告)号：US10071364B2

公开(公告)日：2018-09-11

申请号：US14598401

申请日：2015-01-16

Applicant: Genzyme Corporation

Inventor： Rahul Godawat ,  Veena Warikoo ,  Rohan Patil ,  Konstantin Konstantinov ,  Venkat Kishore Ryakala

IPC: A61L2/08 ,  B01J20/34 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/281 ,  B01J41/12 ,  B01J41/20 ,  C07K1/16 ,  C07K1/22 ,  C07K1/18 ,  B01D15/18 ,  B01D15/20 ,  B01D15/32 ,  B01D15/34 ,  B01J39/26

CPC classification number: B01J20/3441 ,  A61L2/081 ,  B01D15/1864 ,  B01D15/20 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/265 ,  B01J20/281 ,  B01J39/26 ,  B01J41/12 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  Y10T29/49826

Abstract: Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.

公开(公告)号：US10000751B2

公开(公告)日：2018-06-19

申请号：US15231082

申请日：2016-08-08

Applicant: General Electric Company

Inventor： Lin Chen ,  Bing Zhang ,  Klaus Hochleitner ,  Rong Hou ,  Yanju Wang

IPC: C07H21/00 ,  C12N15/10 ,  B01J20/10 ,  B01J20/28 ,  B01J20/281 ,  C07H1/06 ,  C07H1/08 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12N15/1017 ,  B01J20/103 ,  B01J20/28023 ,  B01J20/28038 ,  B01J20/281 ,  B01J2220/82 ,  C07H1/06 ,  C07H1/08 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

Abstract: A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.

公开(公告)号：US09833768B2

公开(公告)日：2017-12-05

申请号：US15661754

申请日：2017-07-27

Applicant: Impossible Foods Inc. ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Patrick O'Reilly Brown ,  R. Edward Watts ,  Pehr Harbury

IPC: C07D251/70 ,  C07D401/04 ,  C07D401/14 ,  C07D403/04 ,  C07D403/14 ,  C07D413/04 ,  C07D413/14 ,  A61K31/53 ,  C07K1/22 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  C07D251/54 ,  G01N33/68 ,  G01N33/543 ,  G01N33/28

CPC classification number: B01J20/281 ,  A61K31/53 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3248 ,  B01J20/3251 ,  C07D251/54 ,  C07D251/70 ,  C07K1/22 ,  G01N33/543 ,  G01N33/6818

Abstract: Disclosed herein are methods and compositions for purifying proteins from crude solutions.

公开(公告)号：US09827552B2

公开(公告)日：2017-11-28

申请号：US14333561

申请日：2014-07-17

Applicant: Clemson University

Inventor： R. Kenneth Marcus ,  Kenneth A. Christensen

IPC: B01J20/00 ,  B01J20/281 ,  G01N30/50 ,  G01N33/543 ,  B01J20/28 ,  B01J20/285 ,  B01J20/32 ,  B01D15/20 ,  B01D15/38 ,  C07K1/20 ,  G01N30/88

CPC classification number: B01J20/281 ,  B01D15/206 ,  B01D15/3823 ,  B01J20/28023 ,  B01J20/28052 ,  B01J20/285 ,  B01J20/321 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/56 ,  C07K1/20 ,  G01N30/50 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2030/8813 ,  Y10T436/255

Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.