公开(公告)号：US20230213527A1

公开(公告)日：2023-07-06

申请号：US18145815

申请日：2022-12-22

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Manjula Pandey ,  David Kamber ,  Kenneth Skinner

IPC: G01N33/68

CPC classification number: G01N33/6818 ,  G01N33/6893 ,  G01N2440/12 ,  G01N2440/18 ,  G01N2440/10 ,  G01N2440/14 ,  G01N2800/102 ,  G01N2800/2821 ,  G01N2800/32

Abstract: Aspects of the application relate to methods and systems for obtaining information regarding multiple amino acids in a polypeptide based on binding interactions between the polypeptide and one or more amino acid recognizers. Kinetic signature information may be obtained from a series of signal pulses indicative of a series of binding events between one or more amino acid recognizers and an amino acid of a polypeptide (e.g., a terminal amino acid, an internal amino acid). The kinetic signature information (e.g., pulse duration, interpulse duration, recognition segment (RS) duration, intersegment duration) may be used to determine one or more chemical characteristics (e.g., identity, modification) of multiple amino acids of the polypeptide.

公开(公告)号：US11655504B2

公开(公告)日：2023-05-23

申请号：US16043547

申请日：2018-07-24

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC: C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6806 ,  C07H19/073 ,  C07H21/04 ,  C07H19/173 ,  C12Q1/6869

CPC classification number: C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2525/185 ,  C12Q2525/197 ,  C12Q2525/313 ,  C12Q2563/103 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/103 ,  C12Q2565/102 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US11613772B2

公开(公告)日：2023-03-28

申请号：US16751138

申请日：2020-01-23

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC: C12Q1/68 ,  G01N21/64 ,  C12Q1/6818 ,  C12Q1/6869

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US20230058852A1

公开(公告)日：2023-02-23

申请号：US17690920

申请日：2022-03-09

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg ,  Thomas Christian

IPC: C12N9/12 ,  C12Q1/6869 ,  C12P19/34 ,  C12N15/52

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

公开(公告)号：US20220186297A1

公开(公告)日：2022-06-16

申请号：US17552339

申请日：2021-12-15

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  An Li ,  Omer Ad ,  Haidong Huang ,  Guojun Chen

IPC: C12Q1/6823 ,  C12Q1/6837 ,  C12Q1/6876 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804

Abstract: Methods and devices for ultrasensitive detection of target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices enable ultrasensitive determination of the concentration of target molecules.

公开(公告)号：US20220186295A1

公开(公告)日：2022-06-16

申请号：US17300940

申请日：2021-12-15

Applicant: Quantum-Si Incorporated

Inventor： Omer Ad ,  Robert E. Boer ,  Evan McCormack ,  Brianna Leigh Haining ,  Thomas Raymond Thurston ,  Brian Reed

IPC: C12Q1/6816

Abstract: Aspects of the disclosure provide methods of determining molecular barcode content based on binding interactions between a barcode recognition molecule and a molecular barcode. In some aspects, the disclosure relates to methods comprising contacting a molecular barcode with a barcode recognition molecule that binds to one or more sites on the molecular barcode, detecting a series of signal pulses, and determining the barcode content based on a barcode-specific pattern in the series of signal pulses.

公开(公告)号：US20220128469A1

公开(公告)日：2022-04-28

申请号：US17511249

申请日：2021-10-26

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Kyle Preston ,  Xinghua Shi ,  Gerard Schmid

IPC: G01N21/64 ,  G01N33/68

Abstract: Aspects of the present disclosure relate to techniques for calibrating a system comprising integrated device. According to some embodiments, there is provided a method for calibrating a system comprising an integrated device, the method comprising: exciting, with light from at least one excitation source, a reference dye molecule disposed in a chamber of the integrated device; obtaining a signal emitted by the reference dye molecule, the signal containing information representative of a bleaching time of the reference dye molecule; and adjusting one or more characteristics of the system based on the bleaching time of the reference dye molecule.

公开(公告)号：US20210354133A1

公开(公告)日：2021-11-18

申请号：US17236804

申请日：2021-04-21

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  John H. Leamon ,  Jonathan C. Schultz ,  Michele Millham ,  Caixia Lv ,  Haidong Huang ,  Roger Nani ,  Omer Ad ,  Brian Reed ,  Matthew Dyer ,  Robert E. Boer

IPC: B01L3/00 ,  C12Q1/6806

Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).

公开(公告)号：US20210148922A1

公开(公告)日：2021-05-20

申请号：US17082940

申请日：2020-10-28

Applicant: Quantum-Si Incorporated

Inventor： Matthew Dyer ,  Brian Reed

IPC: G01N33/68

Abstract: Provided herein are methods of single-polypeptide sequencing and reconstruction. Also provided herein are compositions, kits and devices useful for the same.

公开(公告)号：US20210139973A1

公开(公告)日：2021-05-13

申请号：US17082918

申请日：2020-10-28

Applicant: Quantum-Si Incorporated

Inventor： Matthew Dyer ,  Brian Reed

IPC: C12Q1/6869

Abstract: Provided herein are methods of single-cell polypeptide and/or polynucleic acid sequencing, which facilitate the direct sequencing of a single cell without amplification. Also provided herein are compositions, kits and devices useful for the same.