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公开(公告)号：US10457980B2
公开(公告)日：2019-10-29
申请号：US14435735
申请日：2014-04-30
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Long Cai , Eric Lubeck , Timur Zhiyentayev , Ahmet Coskun , Ting-Fang He , Chang Ho Sohn , Sheel Shah
Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.
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2.发明申请MULTIPLEX LABELING OF MOLECULES BY SEQUENTIAL HYBRIDIZATION BARCODING USING PROBES WITH CLEAVABLE LINKERS 审中-公开通过使用具有可清除链接的探针进行序列杂交化修饰分子的多重标记公开(公告)号：US20160369329A1
公开(公告)日：2016-12-22
申请号：US15225820
申请日：2016-08-01
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Long CAI , Chee Huat Eng , Eric Lubeck
CPC classification number: C12Q1/6841 , C12Q2537/143 , C12Q2563/107
Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, nucleic acid probes include a signal moiety connected with a binding sequence via a cleavable linker.
Abstract translation: 本发明尤其提供了用于在细胞，组织，器官或生物体中检测和/或定量核酸的技术。 通过顺序条码，本发明提供了用于大量目标（例如转录物和/或DNA基因座）的高通量分析的方法。 在一些实施方案中，核酸探针包括通过可切割接头与结合序列连接的信号部分。
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Patent Agency Ranking
3.发明申请公开(公告)号：US20150267251A1
公开(公告)日：2015-09-24
申请号：US14435735
申请日：2014-04-30
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Long Cai , Eric Lubeck , Timur Zhiyentayev , Ahmet Coskun , Ting-Fang He , Chang Ho Sohn , Sheel Shah
CPC classification number: C12Q1/6841 , C12N15/1065 , G01N21/6428 , G01N21/6458 , G01N2201/068 , G01N2201/10 , G02B21/06 , G02B21/16 , C12Q2537/143 , C12Q2565/102 , C12Q2565/601
Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.
Abstract translation: 本发明尤其提供了用于在细胞，组织，器官或生物体中检测和/或定量核酸的技术。 在一些实施方案中，通过顺序条码，本发明提供了用于大量目标（例如转录物和/或DNA基因座）的高通量分析的方法。
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4.发明申请MULTIPLEX DETECTION OF MOLECULAR SPECIES IN CELLS BY SUPER-RESOLUTION IMAGING AND COMBINATORIAL LABELING 审中-公开通过超分辨率成像和组合标签检测细胞中分子物种的多重检测公开(公告)号：US20140031243A1
公开(公告)日：2014-01-30
申请号：US13725717
申请日：2012-12-21
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Long Cai , Eric Lubeck
IPC: C12Q1/68
CPC classification number: C12Q1/6809 , C12Q1/6841 , C12Q2543/10
Abstract: Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super-resolution technologies such as super-resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcodes can be resolved. In addition, alternative splicing events can be characterized by identifying and quantifying mRNA isoforms in an individual cell.
Abstract translation: 提供了方法和系统，用于为单细胞内的细胞成分创建分子条形码或标记，并用超分辨率显微镜等超分辨率技术解决此类条形码或标记。 通过这种方法，可以在单个细胞中同时测量的许多分子种类。 已经证明可以用空间有序的荧光团序列标记多个mRNA转录物，并且可以解析条形码。 此外，可以通过鉴定和定量单个细胞中的mRNA同种型来表征可选剪接事件。
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公开(公告)号：US11473129B2
公开(公告)日：2022-10-18
申请号：US16572511
申请日：2019-09-16
Applicant: California Institute of Technology
Inventor： Long Cai , Eric Lubeck , Timur Zhiyentayev , Ahmet Coskun , Ting-Fang He , Chang Ho Sohn , Sheel Shah
IPC: C12Q1/6841 , C12N15/10 , G01N21/64 , G02B21/06 , G02B21/16
Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.
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公开(公告)号：US10510435B2
公开(公告)日：2019-12-17
申请号：US15298219
申请日：2016-10-19
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Long Cai , Sheel Shah , Eric Lubeck , Wen Zhou
IPC: C12Q1/68 , C07H21/00 , G16B25/00 , G01N21/78 , G16B40/00 , C12Q1/6841 , G01N21/64 , C12Q1/6881
Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.
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公开(公告)号：US10266876B2
公开(公告)日：2019-04-23
申请号：US13725717
申请日：2012-12-21
Applicant: California Institute of Technology
Inventor： Long Cai , Eric Lubeck
IPC: C12Q1/68 , C12Q1/6809 , C12Q1/6841
Abstract: Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super-resolution technologies such as super-resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcodes can be resolved. In addition, alternative splicing events can be characterized by identifying and quantifying mRNA isoforms in an individual cell.
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