公开(公告)号：US20160369329A1

公开(公告)日：2016-12-22

申请号：US15225820

申请日：2016-08-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Chee Huat Eng ,  Eric Lubeck

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12Q2537/143 ,  C12Q2563/107

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, nucleic acid probes include a signal moiety connected with a binding sequence via a cleavable linker.

Abstract translation: 本发明尤其提供了用于在细胞，组织，器官或生物体中检测和/或定量核酸的技术。 通过顺序条码，本发明提供了用于大量目标（例如转录物和/或DNA基因座）的高通量分析的方法。 在一些实施方案中，核酸探针包括通过可切割接头与结合序列连接的信号部分。

公开(公告)号：US20160019334A1

公开(公告)日：2016-01-21

申请号：US14802919

申请日：2015-07-17

Applicant: California Institute of Technology

Inventor： Long CAI ,  Ahmet F. COSKUN

IPC: G06F19/12 ,  G06F19/26 ,  C40B30/02

CPC classification number: G16B5/00 ,  G16B35/00 ,  G16B45/00 ,  G16C20/60

Abstract: Disclosed herein are methods and systems for analyzing visual data from multiple rounds of hybridization interactions where the same molecular target is detected by probes with different detectable labels. In particular, disclosed herein are methods and systems for analyzing sequential hybridization images for molecular profiling, where the images are obtained using multiplex fluorescence in situ hybridization (FISH).

Abstract translation: 本文公开了用于分析来自多轮杂交相互作用的视觉数据的方法和系统，其中通过具有不同可检测标记的探针检测相同的分子靶。 特别地，本文公开了用于分析用于分子谱分析的顺序杂交图像的方法和系统，其中使用多重荧光原位杂交（FISH）获得图像。

公开(公告)号：US20240141326A1

公开(公告)日：2024-05-02

申请号：US18278586

申请日：2022-02-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Daniel DAR ,  Dianne K. NEWMAN ,  Kirsten FRIEDA ,  Long CAI

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12N15/1055 ,  C12N15/1058

Abstract: Method for detecting and barcoding the molecular changes occurring in two or more samples upon exposure to different stimuli. This disclosure herein sets for methods that allow a targeted transcriptome-imaging approach that records both gene-expression and spatial context within microscale assemblies at a single-cell and molecule resolution on biological samples. This disclosure herein sets for methods that allows the application to a variety of biological samples for the study of cellular processes, growth, and interactions between biological samples.

公开(公告)号：US20230332214A1

公开(公告)日：2023-10-19

申请号：US18074369

申请日：2022-12-02

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Carsten H. TISCHBIREK

IPC: C12Q1/6841 ,  G01N33/58

CPC classification number: C12Q1/6841 ,  G01N33/58

Abstract: This disclosure herein sets forth embodiments for a method for mapping the spatial distribution of one or more cellular components or cellular interactions by using barcodes to identify a cell or cellular component in the one or more cells, localizing, detecting, and mapping the spatial distribution of the one or more cellular components. The disclosure herein sets forth method to allow the mapping of spatial distributions of cellular components both intracellularly or intercellularly.

公开(公告)号：US20210395804A1

公开(公告)日：2021-12-23

申请号：US17352162

申请日：2021-06-18

Applicant: California Institute of Technology

Inventor： Long CAI ,  Chee Huat (Linus) ENG ,  James J. PARK ,  Yujing YANG ,  Michal POLONSKY

IPC: C12Q1/6837 ,  C12Q1/6841 ,  G01N33/68 ,  G01N33/543

Abstract: This disclosure herein sets forth embodiments to provide a serological test to detect target analytes that can scale to up to 10,000 or more samples in a single run. This disclosure herein sets forth methods to allow for unique barcoding by using a multi-level barcode scheme that is modular and enables easy detection of multiple analytes in samples.

公开(公告)号：US20250043340A1

公开(公告)日：2025-02-06

申请号：US18906096

申请日：2024-10-03

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Eric LUBECK ,  Timur ZHIYENTAYEV ,  Ahmet COSKUN ,  Ting-Fang HE ,  Chang Ho SOHN ,  Sheel SHAH

IPC: C12Q1/6841 ,  C12N15/10 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

公开(公告)号：US20240384331A1

公开(公告)日：2024-11-21

申请号：US18555204

申请日：2022-04-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Yodai TAKEI

IPC: C12Q1/6841 ,  C12Q1/6876

Abstract: The present disclosure provides methods for analyzing genomic structures by diffraction limited locus imaging and nucleic acid block coding. The methods allow efficient and scalable imaging, which can be applied to multiplexed RNA/DNA fluorescence in situ hybridization (FISH).

公开(公告)号：US20210180127A1

公开(公告)日：2021-06-17

申请号：US17103757

申请日：2020-11-24

Applicant: California Institute of Technology

Inventor： Long CAI ,  Chang Ho SOHN ,  Yandong ZHANG

IPC: C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6806 ,  C12Q1/6825

Abstract: The present application describes compositions and methods for identifying and quantitating molecular targets within a cellular environment. Specifically, provided herein are compositions and methods for separately identifying and quantifying each of one or more molecular targets from a single cell. More specifically, provided herein are compositions and methods for separately identifying and quantifying the same molecular target from a single cell.

公开(公告)号：US20210115504A1

公开(公告)日：2021-04-22

申请号：US16770889

申请日：2018-12-07

Applicant: California Institute of Technology

Inventor： Long CAI ,  Yodai TAKEI

IPC: C12Q1/6841 ,  C12Q1/6827

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, nucleic acid probes include a signal moiety connected with a binding sequence via a cleavable linker.

公开(公告)号：US20170212983A1

公开(公告)日：2017-07-27

申请号：US15298219

申请日：2016-10-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Sheel SHAH ,  Eric LUBECK ,  Wen ZHOU

IPC: G06F19/20 ,  G01N21/78 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/24

Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.