公开(公告)号：US20250043340A1

公开(公告)日：2025-02-06

申请号：US18906096

申请日：2024-10-03

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Eric LUBECK ,  Timur ZHIYENTAYEV ,  Ahmet COSKUN ,  Ting-Fang HE ,  Chang Ho SOHN ,  Sheel SHAH

IPC: C12Q1/6841 ,  C12N15/10 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

公开(公告)号：US20170212983A1

公开(公告)日：2017-07-27

申请号：US15298219

申请日：2016-10-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Sheel SHAH ,  Eric LUBECK ,  Wen ZHOU

IPC: G06F19/20 ,  G01N21/78 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/24

Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.

公开(公告)号：US20230295697A1

公开(公告)日：2023-09-21

申请号：US17940650

申请日：2022-09-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Eric LUBECK ,  Timur ZHIYENTAYEV ,  Ahmet COSKUN ,  Ting-Fang HE ,  Chang Ho SOHN ,  Sheel SHAH

IPC: C12Q1/6841 ,  C12N15/10 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  G01N21/6428 ,  G01N21/6458 ,  G02B21/06 ,  G02B21/16 ,  G01N2201/068 ,  G01N2201/10

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

公开(公告)号：US20210017587A1

公开(公告)日：2021-01-21

申请号：US16322462

申请日：2017-08-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Chee Huat ENG ,  Sheel SHAH

IPC: C12Q1/6841

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Pre-designed barcodes are associated specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is developed to overcome limitations in the previous generation of the technology such as lack of visual signals that can be associated with the probes or small internal within cell when carrying out in situ experiments. The current method can be applied to both in vitro and in situ analysis. According to the method, each barcoding round comprises multiple serial hybridizations where a small number of colored signals (that are associated with probes) are used in each hybridization experiment within a serial hybridization round. Images from each serial hybridization experiment within the same serial hybridization round are combined to form a composite image for each barcoding round. In each barcoding round, the same set of molecular targets are analyzed. After all barcoding rounds are completed, associated of the barcode with these molecular targets is completed.

公开(公告)号：US20230212658A1

公开(公告)日：2023-07-06

申请号：US17940356

申请日：2022-09-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Long CAI ,  Eric LUBECK ,  Timur ZHIYENTAYEV ,  Ahmet COSKUN ,  Ting-Fang HE ,  Chang Ho SOHN ,  Sheel SHAH

IPC: C12Q1/6841 ,  G01N21/64 ,  G02B21/16 ,  G02B21/06 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  G01N21/6428 ,  G02B21/16 ,  G01N21/6458 ,  G02B21/06 ,  C12N15/1065 ,  G01N2201/068 ,  G01N2201/10

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

公开(公告)号：US20200080139A1

公开(公告)日：2020-03-12

申请号：US16572511

申请日：2019-09-16

Applicant: California Institute of Technology

Inventor： Long CAI ,  Eric LUBECK ,  Timur ZHIYENTAYEV ,  Ahmet COSKUN ,  Ting-Fang HE ,  Chang Ho SOHN ,  Sheel SHAH

IPC: C12Q1/6841 ,  C12N15/10 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.