公开(公告)号：US10734212B2

公开(公告)日：2020-08-04

申请号：US15108203

申请日：2014-12-20

Applicant: DH Technologies Development PTE Ltd.

Inventor： Robert Haufler ,  William M. Loyd ,  Alexander Tsipirovich

IPC: H01J49/00 ,  H01J49/06 ,  H05H5/06 ,  H01J49/40 ,  H01J49/02 ,  H01J49/26

Abstract: A ring stacked accelerator for use in a mass spectrometer includes a plurality of ring shaped plates arranged in a stack and is electrically coupled to a voltage divider that allows a substantially homogeneous electric field to be produced when the stack is energized. The voltage divider can include resistors and capacitors, where the capacitors are chosen to compensate for parasitic capacitances experienced by the plates. The ring stacked accelerator can be energized using an RF pulse. The ring stack accelerator can include one or more balancing capacitors for correcting effects that cause nonlinearity.

公开(公告)号：US20210082678A1

公开(公告)日：2021-03-18

申请号：US17041324

申请日：2019-04-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/40 ,  G01N33/68 ,  G16B40/10

Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. M An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

公开(公告)号：US11728148B2

公开(公告)日：2023-08-15

申请号：US17650836

申请日：2022-02-11

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/00

CPC classification number: H01J49/0031 ,  H01J49/0036 ,  H01J49/0054 ,  H01J49/0059 ,  H01J49/0072

Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

公开(公告)号：US20220375736A1

公开(公告)日：2022-11-24

申请号：US17650836

申请日：2022-02-11

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/00 ,  H01J3/40 ,  H01J29/84 ,  H01J49/06

Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

公开(公告)号：US20210257200A1

公开(公告)日：2021-08-19

申请号：US17255607

申请日：2019-08-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/00

Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

公开(公告)号：US11948788B2

公开(公告)日：2024-04-02

申请号：US17310989

申请日：2020-06-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Robert E. Haufler ,  William M. Loyd ,  Takashi Baba

IPC: H01J49/40 ,  H01J49/00

CPC classification number: H01J49/40 ,  H01J49/0031 ,  H01J49/0036

Abstract: A calibration apparatus for a mass analyzer includes an ion source device and a dual-purpose electron beam generating unit. The ion source device ionizes an analyte of a sample, producing analyte ions. The dual-purpose electron beam generating unit is positioned between the ion source device and the mass analyzer. In a first mode, the dual-purpose electron beam generating unit is used to create fragments of analyte ions of unknown mass-to-charge ratio. In a second mode, the dual-purpose electron beam generating unit is used to create ions of calibration compounds of known mass-to-charge ratio. All ions are subsequently transferred to the mass analyzer.

公开(公告)号：US11335547B2

公开(公告)日：2022-05-17

申请号：US17041324

申请日：2019-04-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/40 ,  G16B40/10 ,  G01N33/68 ,  G01N33/577 ,  H01J49/00 ,  G16B30/10

Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

公开(公告)号：US20220093383A1

公开(公告)日：2022-03-24

申请号：US17310989

申请日：2020-06-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Robert E. Haufler ,  William M. Loyd ,  Takashi Baba

IPC: H01J49/40 ,  H01J49/00

Abstract: A calibration apparatus for a mass analyzer includes an ion source device and a dual-purpose electron beam generating unit. The ion source device ionizes an analyte of a sample, producing analyte ions. The dual-purpose electron beam generating unit is positioned between the ion source device and the mass analyzer. In a first mode, the dual-purpose electron beam generating unit is used to create fragments of analyte ions of unknown mass-to-charge ratio. In a second mode, the dual-purpose electron beam generating unit is used to create ions of calibration compounds of known mass-to-charge ratio. All ions are subsequently transferred to the mass analyzer.

公开(公告)号：US11251029B2

公开(公告)日：2022-02-15

申请号：US17255607

申请日：2019-08-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/00 ,  H01J3/40 ,  H01J29/84 ,  H01J49/06

Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

公开(公告)号：US20160329201A1

公开(公告)日：2016-11-10

申请号：US15108203

申请日：2014-12-20

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Robert Haufler ,  William M. Loyd ,  Alexander Tsipirovich

IPC: H01J49/06 ,  H01J49/26

Abstract: A ring stacked accelerator for use in a mass spectrometer includes a plurality of ring shaped plates arranged in a stack and is electrically coupled to a voltage divider that allows a substantially homogeneous electric field to be produced when the stack is energized. The voltage divider can include resistors and capacitors, where the capacitors are chosen to compensate for parasitic capacitances experienced by the plates. The ring stacked accelerator can be energized using an RF pulse. The ring stack accelerator can include one or more balancing capacitors for correcting effects that cause nonlinearity.

Abstract translation: 在质谱仪中使用的环叠层促进器包括多个以叠层布置的环形板，并且电耦合到分压器，该分压器允许当堆叠通电时产生基本上均匀的电场。 分压器可以包括电阻器和电容器，其中电容器被选择来补偿由板经历的寄生电容。 环形堆叠加速器可以使用RF脉冲通电。 环形堆叠加速器可以包括一个或多个平衡电容器，用于校正导致非线性的效应。