公开(公告)号：US20200255893A1

公开(公告)日：2020-08-13

申请号：US16598591

申请日：2019-10-10

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874 ,  B01L3/00

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20180245150A1

公开(公告)日：2018-08-30

申请号：US15896572

申请日：2018-02-14

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US10260095B2

公开(公告)日：2019-04-16

申请号：US16007829

申请日：2018-06-13

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20160076097A1

公开(公告)日：2016-03-17

申请号：US14859725

申请日：2015-09-21

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Baghbani Parizi ,  Eric S. Nordman

IPC: C12Q1/68 ,  G01N1/40

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，从具有相关生物材料的其它珠分离的珠粒。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US10787705B2

公开(公告)日：2020-09-29

申请号：US15896572

申请日：2018-02-14

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US10266892B2

公开(公告)日：2019-04-23

申请号：US16007969

申请日：2018-06-13

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US10100356B2

公开(公告)日：2018-10-16

申请号：US14859725

申请日：2015-09-21

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Baghbani Parizi ,  Eric S. Nordman

IPC: C12Q1/6874 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6848 ,  G01N27/414 ,  G01N1/40 ,  B01L3/00 ,  B01L7/00 ,  B03C1/28 ,  B03C5/00 ,  B03C5/02 ,  B03C1/30 ,  G01N33/543 ,  C40B60/10

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

公开(公告)号：US09274077B2

公开(公告)日：2016-03-01

申请号：US13838816

申请日：2013-03-15

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: G01N27/327 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  G01N27/327 ,  G01N33/54313 ,  G01N33/5438 ,  C12Q2527/119 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2549/126

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Abstract translation: 本发明涉及用于测序多核苷酸的系统和方法，以及检测涉及其它生物分子的反应和结合事件。 该系统和方法可以采用无室装置和纳米传感器来检测或表征高通量的这种反应。 因为在许多实施例中的系统是可重复使用的，与单次使用设备相比，该系统可以经受更复杂和改进的工程。

公开(公告)号：US20190177791A1

公开(公告)日：2019-06-13

申请号：US16283544

申请日：2019-02-22

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874 ,  G01N33/543 ,  G01N27/327

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20180327837A1

公开(公告)日：2018-11-15

申请号：US16039016

申请日：2018-07-18

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  G01N27/327 ,  G01N33/54313 ,  G01N33/5438 ,  C12Q2527/119 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2549/126

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.