公开(公告)号：US20200255893A1

公开(公告)日：2020-08-13

申请号：US16598591

申请日：2019-10-10

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874 ,  B01L3/00

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20180245150A1

公开(公告)日：2018-08-30

申请号：US15896572

申请日：2018-02-14

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20170065977A1

公开(公告)日：2017-03-09

申请号：US15183406

申请日：2016-06-15

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar B. Parizi ,  Mark F. Oldham

IPC: B01L3/00 ,  B01L7/00 ,  B03C5/02 ,  B03C5/00 ,  B03C1/30 ,  C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.

Abstract translation: 本发明的方面包括通过利用未标记的非可合并核苷酸或通过重新构建基于集落的测序反应来提高测序反应的准确性和读取长度的方法。 其他方面包括用于改进与珠粒相关的生物反应测量的系统和装置，其可以通过与所述珠粒相关联的抗衡离子的电流测量方法，由于存在与所述珠粒结合或相关联的反应物，其中用于产生和 测量所述电流可以在所述焊道的德拜长度内。 本发明的其它方面包括用于确定输入样品的浓度的方法，用于重复使用阵列的装置，用于分离具有不同电荷水平的珠子的方法和装置。

公开(公告)号：US10260095B2

公开(公告)日：2019-04-16

申请号：US16007829

申请日：2018-06-13

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20160076097A1

公开(公告)日：2016-03-17

申请号：US14859725

申请日：2015-09-21

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Baghbani Parizi ,  Eric S. Nordman

IPC: C12Q1/68 ,  G01N1/40

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，从具有相关生物材料的其它珠分离的珠粒。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US11155865B2

公开(公告)日：2021-10-26

申请号：US16039016

申请日：2018-07-18

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543 ,  B01L3/00

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US11021748B2

公开(公告)日：2021-06-01

申请号：US16598591

申请日：2019-10-10

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/68 ,  B01L3/00 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20190226021A1

公开(公告)日：2019-07-25

申请号：US16115344

申请日：2018-08-28

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Baghbani Parizi ,  Eric S. Nordman

IPC: C12Q1/6874 ,  B03C1/30 ,  G01N33/543 ,  C12Q1/6869 ,  C12Q1/6848 ,  G01N27/414 ,  B01L7/00 ,  B03C5/02 ,  B01L3/00 ,  G01N1/40 ,  B03C5/00 ,  B03C1/28 ,  C12Q1/6853

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

公开(公告)号：US20190177790A1

公开(公告)日：2019-06-13

申请号：US16283531

申请日：2019-02-22

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T, Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874 ,  G01N33/543 ,  G01N27/327

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US20180282806A1

公开(公告)日：2018-10-04

申请号：US16007969

申请日：2018-06-13

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  G01N27/327 ,  G01N33/54313 ,  G01N33/5438 ,  C12Q2527/119 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2549/126

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.