公开(公告)号：US20230293771A1

公开(公告)日：2023-09-21

申请号：US18016491

申请日：2021-07-16

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  ORGANIZATION OF CELL SHEET TISSUE ENGINEERING REGENERATIVE MEDICINE INITIATIVES

Inventor： Mimi Egami ,  David Grainger ,  Kyungsook Kim ,  Makoto Kondo ,  Travis Maak ,  Teruo Okano ,  Hideaki Sakai

IPC: A61L27/38 ,  C12N5/077 ,  C12N11/02 ,  A61L27/36

CPC classification number: A61L27/3852 ,  C12N5/0655 ,  C12N11/02 ,  A61L27/3817 ,  A61L27/3691 ,  C12N2509/00 ,  C12N2533/90 ,  C12N2513/00 ,  C12N2533/54 ,  A61L2430/06

Abstract: The disclosure provides a chondrocyte cell sheet comprising one or more layers of confluent cells comprising chondrocytes and chondroprogenitor cells. Methods of generating cartilage tissue in a subject are also provided. The disclosure also provides a method for producing chondrocyte cell sheets comprising culturing chondrocytes and chondroprogenitor cells in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.

公开(公告)号：US09889235B2

公开(公告)日：2018-02-13

申请号：US14765662

申请日：2014-02-05

Applicant: University of Utah Research Foundation

Inventor： Amanda Brooks ,  David Grainger ,  Kristofer D. Sinclair ,  Benjamin Brooks

IPC: A61K9/00 ,  A61L27/54 ,  A61L27/58 ,  A61L27/30 ,  A61L27/36 ,  A61K31/7036 ,  A61L27/26 ,  B05D3/00 ,  B29C67/24 ,  B29K1/00 ,  B29L31/00

CPC classification number: A61L27/58 ,  A61K31/7036 ,  A61L27/26 ,  A61L27/306 ,  A61L27/3608 ,  A61L27/54 ,  A61L2300/406 ,  A61L2300/412 ,  A61L2300/62 ,  A61L2430/02 ,  A61L2430/24 ,  B05D3/002 ,  B05D2203/00 ,  B29C67/24 ,  B29K2001/08 ,  B29L2031/7532

Abstract: In one aspect, the invention provides an implantable device comprising a uniform mixture of components including degradable polymer, inorganic bone particulate either natural or synthetic, a drug, and a soluble microporagen. In some embodiments, the uniform mixture further includes a soluble polymer macroporagen. In some embodiments, the uniform mixture is coated with an immobilized outer porous layer comprising or consisting of synthetic or natural inorganic bone granules. In further aspects, the invention provides an implantable device comprising a composite core of degradable polymer, bone, and a drug, and a coating comprising or consisting of microporous bone overlayer covering the degradable composite core.

公开(公告)号：US20160129157A1

公开(公告)日：2016-05-12

申请号：US14765662

申请日：2014-02-05

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： Amanda Brooks ,  David Grainger ,  Kristofer D. Sinclair ,  Benjamin Brooks

IPC: A61L27/58 ,  A61L27/26 ,  B05D3/00 ,  A61L27/36 ,  B29C67/24 ,  A61K31/7036 ,  A61L27/54

CPC classification number: A61L27/58 ,  A61K31/7036 ,  A61L27/26 ,  A61L27/306 ,  A61L27/3608 ,  A61L27/54 ,  A61L2300/406 ,  A61L2300/412 ,  A61L2300/62 ,  A61L2430/02 ,  A61L2430/24 ,  B05D3/002 ,  B05D2203/00 ,  B29C67/24 ,  B29K2001/08 ,  B29L2031/7532

Abstract: In one aspect, the invention provides an implantable device comprising a uniform mixture of components including degradable polymer, inorganic bone particulate either natural or synthetic, a drug, and a soluble microporagen. In some embodiments, the uniform mixture further includes a soluble polymer macroporagen. In some embodiments, the uniform mixture is coated with an immobilized outer porous layer comprising or consisting of synthetic or natural inorganic bone granules. In further aspects, the invention provides an implantable device comprising a composite core of degradable polymer, bone, and a drug, and a coating comprising or consisting of microporous bone overlayer covering the degradable composite core.

Abstract translation: 一方面，本发明提供了一种可植入装置，其包括均匀的组分混合物，包括可降解聚合物，天然或合成的无机骨颗粒，药物和可溶性微孔。 在一些实施方案中，均匀混合物还包含可溶性聚合物大分子。 在一些实施方案中，均匀混合物用包含合成或天然无机骨颗粒的固定的外多孔层涂覆。 在另外的方面，本发明提供了一种可植入装置，其包括可降解聚合物，骨骼和药物的复合芯，以及包含可微聚骨覆盖层或覆盖可降解复合芯的或由其组成的涂层。

公开(公告)号：US20230357724A1

公开(公告)日：2023-11-09

申请号：US17422677

申请日：2020-01-15

Applicant: University of Utah Research Foundation

Inventor： Teruo Okano ,  Kyungsook Kim ,  David Grainger

IPC: C12N5/0775

CPC classification number: C12N5/0665 ,  C12N2500/98 ,  C12N2523/00 ,  C12N2539/10

Abstract: The disclosure provides a human umbilical cord mesenchymal stem cell sheet comprising one or more layers of confluent human umbilical cord mesenchymal stem cells (hUC-MSCs). The disclosure also provides method for producing hUC-MSC sheets comprising culturing hUC-MSCs in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.

公开(公告)号：US20210386790A1

公开(公告)日：2021-12-16

申请号：US17422646

申请日：2020-01-15

Applicant: University of Utah Research Foundation

Inventor： Goro Kuramoto ,  Teruo Okano ,  Robert Silver ,  David Grainger

IPC: A61K35/28 ,  A61K35/51 ,  A61L27/38

Abstract: The disclosure provides a method of reducing formation of fibrotic tissue in a uterus of a subject in need thereof, comprising applying a mesenchymal stem cell (MSC) sheet to the uterus of the subject, wherein the MSC sheet comprises one or more layers of aggregated confluent mesenchymal stem cells (MSCs), and wherein applying the MSC sheet to the uterus reduces the formation of fibrotic tissue in the uterus relative to a uterus in which the MSC sheet is not applied. The disclosure also provides a method of increasing myometrial regeneration in a uterus of a subject in need thereof, comprising applying a mesenchymal stem cell (MSC) sheet to the uterus of the subject, wherein the MSC sheet comprises one or more layers of aggregated confluent mesenchymal stem cells (MSCs), and wherein applying the MSC sheet to the uterus increases myometrial regeneration relative to a uterus in which the MSC sheet is not applied.