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Patent Agency Ranking
公开(公告)号：US20220175847A1
公开(公告)日：2022-06-09
申请号：US17435885
申请日：2020-03-05
Applicant: University of Utah Research Foundation
Inventor： Olga Baker , Teruo Okano , Kihoon Nam , Kyungsook Kim
Abstract: The disclosure provides a salivary gland (SG) cell sheet comprising one or more layers of confluent SG cells (e.g. submandibular gland (SMG) cells). Methods of treating a wound in a salivary gland (SG), treating hyposalivation, and treating irradiation damage in an SG are also provided. The disclosure also provides a method for producing SG cell sheets comprising culturing SG cells in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of el the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.
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公开(公告)号：US20230357724A1
公开(公告)日：2023-11-09
申请号：US17422677
申请日：2020-01-15
Applicant: University of Utah Research Foundation
Inventor： Teruo Okano , Kyungsook Kim , David Grainger
IPC: C12N5/0775
CPC classification number: C12N5/0665 , C12N2500/98 , C12N2523/00 , C12N2539/10
Abstract: The disclosure provides a human umbilical cord mesenchymal stem cell sheet comprising one or more layers of confluent human umbilical cord mesenchymal stem cells (hUC-MSCs). The disclosure also provides method for producing hUC-MSC sheets comprising culturing hUC-MSCs in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.
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公开(公告)号：US20210386790A1
公开(公告)日：2021-12-16
申请号：US17422646
申请日：2020-01-15
Applicant: University of Utah Research Foundation
Inventor： Goro Kuramoto , Teruo Okano , Robert Silver , David Grainger
Abstract: The disclosure provides a method of reducing formation of fibrotic tissue in a uterus of a subject in need thereof, comprising applying a mesenchymal stem cell (MSC) sheet to the uterus of the subject, wherein the MSC sheet comprises one or more layers of aggregated confluent mesenchymal stem cells (MSCs), and wherein applying the MSC sheet to the uterus reduces the formation of fibrotic tissue in the uterus relative to a uterus in which the MSC sheet is not applied. The disclosure also provides a method of increasing myometrial regeneration in a uterus of a subject in need thereof, comprising applying a mesenchymal stem cell (MSC) sheet to the uterus of the subject, wherein the MSC sheet comprises one or more layers of aggregated confluent mesenchymal stem cells (MSCs), and wherein applying the MSC sheet to the uterus increases myometrial regeneration relative to a uterus in which the MSC sheet is not applied.
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公开(公告)号：US20240052313A1
公开(公告)日：2024-02-15
申请号：US17766292
申请日：2020-10-07
Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION
Inventor： Teruo Okano , Kyungsook Kim , Hallie Thorp , David W. Grainger , Travis Maak
CPC classification number: C12N5/0655 , A61P19/02 , C12N2506/1353 , C12N2533/54 , C12N2501/155 , C12N2501/60 , C12N2501/998 , C12N2501/15
Abstract: The disclosure relates to a chondrogenic cell sheet comprising at least two layers of confluent chondrogenically differentiated cells, wherein the cell sheet is prepared from mesenchymal stem cells (MSCs), and wherein the chondrogenically differentiated cells on the basal side of the cell sheet express adhesion molecules. The disclosure also relates to a method of preparing a chondrogenic cell sheet comprising: a) culturing MSCs on temperature responsive cultureware until confluent to form a cell sheet; b) detaching the cell sheet by temperature reduction and allowing the cell sheet to contract, forming a contracted cell sheet; c) contacting the contracted cell sheet with a culture surface; and d) treating the contracted cell sheet on the culture surface with chondrogenic medium and culturing to form a chondrogenic cell sheet. Methods of using the chondrogenic cell sheets to repair cartilage tissue or treat joint disease are also disclosed.
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公开(公告)号：US20230293771A1
公开(公告)日：2023-09-21
申请号：US18016491
申请日：2021-07-16
Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION , ORGANIZATION OF CELL SHEET TISSUE ENGINEERING REGENERATIVE MEDICINE INITIATIVES
Inventor： Mimi Egami , David Grainger , Kyungsook Kim , Makoto Kondo , Travis Maak , Teruo Okano , Hideaki Sakai
CPC classification number: A61L27/3852 , C12N5/0655 , C12N11/02 , A61L27/3817 , A61L27/3691 , C12N2509/00 , C12N2533/90 , C12N2513/00 , C12N2533/54 , A61L2430/06
Abstract: The disclosure provides a chondrocyte cell sheet comprising one or more layers of confluent cells comprising chondrocytes and chondroprogenitor cells. Methods of generating cartilage tissue in a subject are also provided. The disclosure also provides a method for producing chondrocyte cell sheets comprising culturing chondrocytes and chondroprogenitor cells in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.
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