公开(公告)号：US09625470B2

公开(公告)日：2017-04-18

申请号：US13888841

申请日：2013-05-07

Applicant: Wisconsin Alumni Research Foundation

Inventor： Joshua Jacques Coon ,  Michael Scott Westphall ,  Derek James Bailey

IPC: G06F19/00 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/6848 ,  H01J49/0031 ,  H01J49/0036

Abstract: A method of identifying a related peak set from MS1 spectra data is provided. An intensity peak is selected from MS1 spectra data generated for a sample by a tandem mass spectrometer. A peak location is identified for the selected intensity peak. An intensity score is calculated from the MS1 spectra data for each of a plurality of possible related peak locations based on an intensity value associated with each of the plurality of possible related peak locations. Whether or not any of the plurality of possible related peak locations forms a related peak set is determined based on the calculated intensity score for each of the plurality of possible related peak locations.

公开(公告)号：US20130292581A1

公开(公告)日：2013-11-07

申请号：US13927650

申请日：2013-06-26

Applicant: Wisconsin Alumni Research Foundation

Inventor： Jason Dale Russell ,  Joshua Jacques Coon ,  Ryan Tyler Hilger ,  Lloyd Michael Smith ,  Daniel T. Ladror ,  Michael Robert Shortreed ,  Mark Andrew Scalf

IPC: H01J49/16

CPC classification number: H01J49/167 ,  G01N21/6486 ,  G01N30/7266

Abstract: An integrated mass spectrometer electrospray emitter and fluorescence detector allows improved volumetric measurements of separate components from a liquid chromatography column by improving correlation between the readings of these instruments and reducing dead volume and sample size requirements.

Abstract translation: 集成的质谱仪电喷射发射器和荧光检测器可以通过改善这些仪器的读数之间的相关性，减少死体积和样品量要求，从而提高液相色谱柱分离成分的体积测量。

公开(公告)号：US09620348B2

公开(公告)日：2017-04-11

申请号：US13927650

申请日：2013-06-26

Applicant: Wisconsin Alumni Research Foundation

Inventor： Jason Dale Russell ,  Joshua Jacques Coon ,  Ryan Tyler Hilger ,  Lloyd Michael Smith ,  Daniel T. Ladror ,  Michael Robert Shortreed ,  Mark Andrew Scalf

IPC: H01J49/00 ,  G01N21/64 ,  H01J49/16 ,  G01N21/00

CPC classification number: H01J49/167 ,  G01N21/6486 ,  G01N30/7266

Abstract: An integrated mass spectrometer electrospray emitter and fluorescence detector allows improved volumetric measurements of separate components from a liquid chromatography column by improving correlation between the readings of these instruments and reducing dead volume and sample size requirements.

公开(公告)号：US11728146B2

公开(公告)日：2023-08-15

申请号：US17575135

申请日：2022-01-13

Applicant: Wisconsin Alumni Research Foundation

Inventor： Michael Scott Westphall ,  Joshua Jacques Coon

IPC: G01N1/42 ,  H01J49/04 ,  H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0004 ,  G01N1/42 ,  H01J49/04 ,  H01J49/0468 ,  H01J49/0486 ,  H01J49/0495 ,  H01J49/062

Abstract: Imaging by cryo-electron microscopy (cryo-EM) requires that a sample be encased in an amorphous solid, such as amorphous ice. In current cryo-EM preparation systems, once the sample has been deposited on an EM grid and coated in the amorphous solid, the EM grid must be removed from vacuum and then transferred into the vacuum of the cryo-EM system. As a result, samples deposited on the grid are exposed to damage and contamination. The present invention provides improved EM grid handling systems and devices compatible with advanced cryo-EM sample preparation techniques and which reduce or eliminate exposure of the sample on the grid to atmosphere and elevated temperatures. These methods and devices will also significantly reduce handling time and complexities associated with cryo-EM sample preparation.

公开(公告)号：US20140336951A1

公开(公告)日：2014-11-13

申请号：US13888841

申请日：2013-05-07

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Joshua Jacques Coon ,  Michael Scott Westphall ,  Derek James Bailey

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  H01J49/0031 ,  H01J49/0036

Abstract: A method of identifying a related peak set from MS1 spectra data is provided. An intensity peak is selected from MS1 spectra data generated for a sample by a tandem mass spectrometer. A peak location is identified for the selected intensity peak. An intensity score is calculated from the MS1 spectra data for each of a plurality of possible related peak locations based on an intensity value associated with each of the plurality of possible related peak locations. Whether or not any of the plurality of possible related peak locations forms a related peak set is determined based on the calculated intensity score for each of the plurality of possible related peak locations.

Abstract translation: 提供了从MS1光谱数据中识别相关峰集的方法。 从通过串联质谱仪为样品生成的MS1光谱数据中选择强度峰值。 识别所选强度峰值的峰值位置。 基于与多个可能的相关峰值位置中的每一个相关联的强度值，从多个可能的相关峰值位置中的每一个的MS1频谱数据计算强度分数。 基于所计算的多个可能的相关峰值位置中的每个可能的相关峰值位置的强度分​​数来确定形成相关峰值集合的多个可能的相关峰值位置中的任一个是否被确定。