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1.发明公开유기용매에 민감한 대장균(Ｅ.ｃｏｌｉ) ＳＢ012 ＫＦＣＣ-11203 및 그 제조방법 失效MICRORGANISM E.CEL SB012（KFCC 11203）对有机溶剂的敏感性及其制备方法
公开(公告)号：KR1020030051067A
公开(公告)日：2003-06-25
申请号：KR1020010081966
申请日：2001-12-20
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: PURPOSE: A microorganism E. coli SB012 (KFCC 11203) sensitive to organic solvents and a method of preparation thereof are provided, thereby detecting toxicity more sensitively. CONSTITUTION: A microorganism E. coli SB012 (KFCC 11203) having improved sensitivity to organic solvents is prepared by modification or deletion of lipopolysaccharide in an extracellular membrane of E. coli SB01, wherein the modification or deletion of lipopolysaccharide in the extracellular membrane of E. coli SB01 is caused by treatment of E. coli SB01 with ethyl-methane-sulfonate(EMS), and repeatedly culturing the EMS-treated E. coli SB01 in a medium containing an antimicrobial agent and an organic solvent, wherein the antimicrobial agent is tetracycline and the organic solvent is para-xylene or bile acid salts.
Abstract translation: 目的：提供对有机溶剂敏感的微生物大肠杆菌SB012（KFCC 11203）及其制备方法，从而更灵敏地检测毒性。 构成：通过在大肠杆菌SB01的细胞外膜中修饰或缺失脂多糖来制备对有机溶剂具有改善的敏感性的微生物大肠杆菌SB012（KFCC 11203），其中在细胞外膜中的脂多糖的修饰或缺失。 大肠杆菌SB01是通过用甲基磺酸乙酯（EMS）处理大肠杆菌SB01引起的，并且在含有抗微生物剂和有机溶剂的培养基中反复培养EMS处理的大肠杆菌SB01，其中抗微生物剂是四环素 有机溶剂为对二甲苯或胆汁酸盐。
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公开(公告)号：KR100379756B1
公开(公告)日：2003-04-11
申请号：KR1020000057916
申请日：2000-10-02
Applicant: 한국과학기술연구원
IPC: C12P41/00
Abstract: PURPOSE: Provided is an effective resolution method of chiral compounds containing hydroxy group or ester group, by using lipase produced by Burkholderia Cocovenenans SY-01I(KFCC 11111). CONSTITUTION: Chiral compounds(formula 1, 2, 3 4 and 5) containing hydroxy group or ester group are resolved by hydrolyzing and trans-esterifying using the solid lipase prepared by dialysis or lyophilizing, wherein the hydrolysis is carried out at 5-35deg.C with by phosphate buffer solution of pH7.09; and the trans-esterification is conducted at 0-55 deg.C with organic solvent and an acylating agent. The organic solvent is selected from n-hexane, ethylacetate, benzene, toluene, THF, diethylether, acetonitrile, dichloroethane, dichloromethane, 1,4-dioxane, methanol, DMSO, DMF and hexane/ethylacetate(9/1 -5/5) and the above acylating agent is chosen from vinylacetate, isopropenylacetate, anhydrous acetic acid, anhydrous propionic acid and anhydrous butyric acid.
Abstract translation: 目的：通过使用由Burkholderia Cocovenenans SY-01I（KFCC 11111）产生的脂肪酶，提供了含有羟基或酯基的手性化合物的有效拆分方法。 构成：含有羟基或酯基的手性化合物（式1,2,3和4）通过使用通过透析或冷冻干燥制备的固体脂肪酶进行水解和酯交换来拆分，其中水解在5-35℃下进行。 C用pH7.09的磷酸盐缓冲溶液; 酯化反应在0-55℃下用有机溶剂和酰化剂进行。 有机溶剂选自正己烷，乙酸乙酯，苯，甲苯，THF，乙醚，乙腈，二氯乙烷，二氯甲烷，1,4-二恶烷，甲醇，DMSO，DMF和己烷/乙酸乙酯（9/1 -5/5） 并且上述酰化剂选自乙酸乙烯酯，异丙烯基乙酸酯，无水乙酸，无水丙酸和无水丁酸。
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公开(公告)号：KR100373151B1
公开(公告)日：2003-02-25
申请号：KR1020000029739
申请日：2000-05-31
Applicant: 한국과학기술연구원 , 한올바이오파마주식회사 , 경동제약 주식회사
IPC: C12N9/16
Abstract: 본발명은 신균주 악시네토박터(
Acinetobacter sp. ) SY-01 (상기 균주는 벌콜데리아 코코베네난스(
Burkholderia cocovenenans ) SY-01로 기탁되었으나, 2002년 5월 1일자로 제출한 과학적 성질 및 분류학상의 위치 표시 등의 기록서를 통하여 분류학상의 위치가
Acinetobacter sp. SY-01로 수정되었음)과 이로부터 생산되는 입체선택적 기질특이성을 갖는 리파제에 관한 것으로, 더욱 상세하게는 항진균제의 원료로 사용되는 이트라코나졸의 중간체로서 라세믹 혼합물인 시스-2-(브로모메틸)-2-(2,4-디클로로 페닐)-1,3-디옥소란-4-메틸 아세테이트의 에스테르결합을 입체선택적으로 분해할 수 있는 리파제에 관한 것이다.-
4.发明公开발광유전자 ｌｕｘＣＤＡＢＥ를 포함하는 재조합플라스미드, 이 플라스미드에 의해 형질 전환된 대장균ＳＢ０１(ＫＦＣＣ １１１７６) 및 이의 용도 无效含有LUXCDABE的重组体PLASMID，大肠杆菌SB01（KFCC 11176）及其用途及其用途
公开(公告)号：KR1020020041603A
公开(公告)日：2002-06-03
申请号：KR1020000071267
申请日：2000-11-28
Applicant: 한국과학기술연구원
IPC: C12N15/65
CPC classification number: C12N15/65 , C12N15/70 , C12Q2600/142
Abstract: PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. CONSTITUTION: The recombinant plasmid pBS1 contains a vector pKLJ12 containing luxCDABE derived from Photorhabdus luminescens. E. coli SB01 KFCC 11176 is produced by transforming E. coli DH5alpha with the recombinant plasmid pBS1. A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution.
Abstract translation: 目的：提供含有luxCDABE，大肠杆菌SB01（KFCC 11176）转化的重组质粒及其用途，因此可以增加重组质粒在大肠杆菌中的稳定性，以用于检测毒性。 构成：重组质粒pBS1含有一种载体pKLJ12，其含有源自Photorhabdus luminescens的luxCDABE。 大肠杆菌SB01KFCC 11176是用重组质粒pBS1转化大肠杆菌DH5α产生的。 用于检测样品溶液中的毒性的方法包括在连续发酵罐中的样品溶液中培养转化的大肠杆菌SB01KFCC 11176，并分析样品溶液的发光。
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公开(公告)号：KR100183360B1
公开(公告)日：1999-04-01
申请号：KR1019970003042
申请日：1997-01-31
Applicant: 한국과학기술연구원
IPC: C02F1/62
Abstract: 저렴한 비용으로 단시간 내에 중금속 폐수 중의 중금속을 제거하는 방법에 관한 것으로서, 이 방법은 종래의 황산염 환원세균에 의한 중금속 제거 방법에 있어서, 종래의 황산염 환원 세균의 생장에 필요한 유기물원으로 사용하던 버섯 배양토를 표고 버섯 재배용 참나무류 원목, 그 재배 후 남은 폐목, 또는 종이 재생 공정에서 부산물로 생산되는 제지 슬러지로 대체하고, 또한 황산염 환원 세균의 농도를 증가시킬 수 있도록 혐기성 소화조 오니 및(또는) 혐기적으로 농화된 미생물원을 상기 유기물원과 함께 사용하는 것을 특징으로 한다.
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Patent Agency Ranking
公开(公告)号：KR100128016B1
公开(公告)日：1998-04-04
申请号：KR1019940015935
申请日：1994-07-04
Applicant: 한국과학기술연구원
IPC: G01L1/24
Abstract: The minute pressure measuring method is comprised of (a) preparing a remote measuring apparatus(2) composing a light source(5), an optical detector(6), an electronic circuit(7) for position detection and an output displaying part(8), and transmitting the light irradiated from the light source through an optical fiber bundle(3) to a place to be measured, then receiving again the light transmitted through a glass tube(1a) with a measuring probe(4) to transmit light to the remote measuring apparatus(2) through the optical fiber bundle; (b) performing real-time tracking the change of a liquid surface level based on light transmitted to the remote measuring apparatus(2), digitalizing it to display the liquid surface level and a minute pressure on the output displaying part(8).
Abstract translation: 微压力测量方法包括：（a）制备构成光源（5）的远程测量装置（2），光学检测器（6），用于位置检测的电子电路（7）和输出显示部分（8） ），并将从光源照射的光通过光纤束（3）传输到待测量的位置，然后再次接收通过测量探针（4）透射通过玻璃管（1a）的光以透射光 远程测量装置（2）通过光纤束; （b）基于传输到所述远程测量装置（2）的光，实时跟踪液面水平的变化，数字化以在所述输出显示部分（8）上显示液面水平和微小压力。
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公开(公告)号：KR100461772B1
公开(公告)日：2004-12-14
申请号：KR1020010081966
申请日：2001-12-20
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: PURPOSE: A microorganism E. coli SB012 (KFCC 11203) sensitive to organic solvents and a method of preparation thereof are provided, thereby detecting toxicity more sensitively. CONSTITUTION: A microorganism E. coli SB012 (KFCC 11203) having improved sensitivity to organic solvents is prepared by modification or deletion of lipopolysaccharide in an extracellular membrane of E. coli SB01, wherein the modification or deletion of lipopolysaccharide in the extracellular membrane of E. coli SB01 is caused by treatment of E. coli SB01 with ethyl-methane-sulfonate(EMS), and repeatedly culturing the EMS-treated E. coli SB01 in a medium containing an antimicrobial agent and an organic solvent, wherein the antimicrobial agent is tetracycline and the organic solvent is para-xylene or bile acid salts.
Abstract translation: 目的：提供对有机溶剂敏感的微生物大肠杆菌SB012（KFCC 11203）及其制备方法，由此更灵敏地检测毒性。 组成：对有机溶剂具有改善的敏感性的微生物大肠杆菌SB012（KFCC 11203）是通过修饰或缺失大肠杆菌SB01的细胞外膜中的脂多糖来制备的，其中E的细胞外膜中的脂多糖的修饰或缺失。 大肠杆菌SB01是通过用乙基 - 甲磺酸盐（EMS）处理大肠杆菌SB01并且在含有抗微生物剂和有机溶剂的培养基中反复培养EMS-处理的大肠杆菌SB01而引起的，其中抗微生物剂是四环素 有机溶剂是对二甲苯或胆汁酸盐。
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公开(公告)号：KR1020040011882A
公开(公告)日：2004-02-11
申请号：KR1020020045165
申请日：2002-07-31
Applicant: 한국과학기술연구원
IPC: C12N15/55
CPC classification number: C12N9/20 , C07K14/212 , C12N15/75 , C12Y301/01003
Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.
Abstract translation: 目的：提供具有立体选择底物特异性的不动杆菌属SY-01的脂肪酶基因和由其表达的蛋白质。 构成：基因的特征在于编码由SEQ ID NO：2表示的不动杆菌属物种SY-01的脂肪酶的氨基酸序列。 通过用含有编码不动杆菌属SY-01的脂肪酶的基因的重组载体质粒pSYLipSM（1）转化获得转化的枯草芽孢杆菌168 / pSYLipSM（1）（KCTC 10267BP）。 通过培养转化的枯草芽孢杆菌并从培养的细胞中分离和纯化不动杆菌属SY-01的表达脂肪酶的步骤制造不动杆菌属物种SY-01的脂肪酶。
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公开(公告)号：KR1019980067148A
公开(公告)日：1998-10-15
申请号：KR1019970003042
申请日：1997-01-31
Applicant: 한국과학기술연구원
IPC: C02F1/62
Abstract: 저렴한 비용으로 단시간 내에 중금속 폐수 중의 중금속을 제거하는 방법에 관한 것으로서, 이 방법은 종래의 황산염 환원 세균에 의한 중금속 제거 방법에 있어서, 종래의 방법에서 황산염 환원 세균의 생장에 필요한 유기물원으로 사용하던 버섯 배양토를 표고 버섯 재배용 참나무류 원목, 그 재배 후 남은 폐목, 또는 종이 재생 공정에서 부산물로 생산되는 제지 슬러지로 대체하고, 또한 황산염 환원 세균의 농도를 증가시킬 수 있도록 혐기성 소화조 오니 및(또는) 혐기적으로 농화된 미생물원을 상기 유기물원과 함께 사용하는 것을 특징으로 한다.
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